YBR204C / LDH1

Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif)

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR204C / LDH1

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:4.24|P-value:5.54E-6 ID:SGTC_46|Compound:0329-0187a|FD-Score:-3.67|P-value:6.37E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:3.22|P-value:4.18E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:3.89|P-value:2.72E-5 ID:SGTC_215|Compound:1287-0124|FD-Score:3.00|P-value:9.33E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.37|P-value:2.42E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:3.21|P-value:4.37E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:4.26|P-value:5.07E-6 ID:SGTC_361|Compound:000s-0510|FD-Score:3.14|P-value:5.77E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:-5.85|P-value:5.39E-10 ID:SGTC_424|Compound:splitomicin|FD-Score:-3.54|P-value:1.09E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.06|P-value:1.29E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:3.20|P-value:4.65E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:3.30|P-value:3.16E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.33|P-value:2.83E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.62|P-value:7.81E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:3.14|P-value:5.68E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.92|P-value:2.35E-5 ID:SGTC_833|Compound:3852-0053|FD-Score:-3.32|P-value:2.59E-4 ID:SGTC_844|Compound:0330-0132|FD-Score:3.93|P-value:2.34E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:3.52|P-value:1.31E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:4.03|P-value:1.44E-5 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.18|P-value:4.97E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.29|P-value:3.22E-4 ID:SGTC_1675|Compound:st018485|FD-Score:-3.90|P-value:2.35E-5 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.20|P-value:4.11E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:9.96|P-value:1.99E-25 ID:SGTC_2428|Compound:5615643|FD-Score:-3.53|P-value:1.12E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-3.00|P-value:8.53E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.36|P-value:2.52E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.15|P-value:5.41E-4 ID:SGTC_3158|Compound:9100007|FD-Score:-3.25|P-value:3.42E-4 ID:SGTC_3162|Compound:9099653|FD-Score:3.12|P-value:6.09E-4 ID:SGTC_3236|Compound:9132701|FD-Score:-3.01|P-value:8.26E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:4.24|P-value:5.54E-6 ID:SGTC_46|Compound:0329-0187a|FD-Score:-3.67|P-value:6.37E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:3.22|P-value:4.18E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:3.89|P-value:2.72E-5 ID:SGTC_215|Compound:1287-0124|FD-Score:3.00|P-value:9.33E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:3.37|P-value:2.42E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:3.21|P-value:4.37E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:4.26|P-value:5.07E-6 ID:SGTC_361|Compound:000s-0510|FD-Score:3.14|P-value:5.77E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:-5.85|P-value:5.39E-10 ID:SGTC_424|Compound:splitomicin|FD-Score:-3.54|P-value:1.09E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.06|P-value:1.29E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:3.20|P-value:4.65E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:3.30|P-value:3.16E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.33|P-value:2.83E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.62|P-value:7.81E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:3.14|P-value:5.68E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.92|P-value:2.35E-5 ID:SGTC_833|Compound:3852-0053|FD-Score:-3.32|P-value:2.59E-4 ID:SGTC_844|Compound:0330-0132|FD-Score:3.93|P-value:2.34E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:3.52|P-value:1.31E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:4.03|P-value:1.44E-5 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.18|P-value:4.97E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.29|P-value:3.22E-4 ID:SGTC_1675|Compound:st018485|FD-Score:-3.90|P-value:2.35E-5 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.20|P-value:4.11E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:9.96|P-value:1.99E-25 ID:SGTC_2428|Compound:5615643|FD-Score:-3.53|P-value:1.12E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-3.00|P-value:8.53E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.36|P-value:2.52E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.15|P-value:5.41E-4 ID:SGTC_3158|Compound:9100007|FD-Score:-3.25|P-value:3.42E-4 ID:SGTC_3162|Compound:9099653|FD-Score:3.12|P-value:6.09E-4 ID:SGTC_3236|Compound:9132701|FD-Score:-3.01|P-value:8.26E-4

Top fitness defect scores for YBR204C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2130 NSC 7 9.96 2.5 µM 1.99E-25 copper-dependent oxidative stress 2 1.09E-23
2 SGTC_360 0957-0399 4.26 1.0 µM 5.07E-6 cell wall 37 1.04E-5
3 SGTC_40 0327-0103 4.24 19.8 µM 5.54E-6 21 1.13E-5
4 SGTC_451 chloral hydrate 4.06 100.0 µM 1.29E-5 47 2.49E-5
5 SGTC_1031 k035-0009 4.03 16.0 µM 1.44E-5 26 2.75E-5
6 SGTC_844 0330-0132 3.93 15.3 µM 2.34E-5 16 4.32E-5
7 SGTC_811 0416-0114 3.92 265.0 µM 2.35E-5 20 4.34E-5
8 SGTC_213 1246-0773 3.89 967.0 nM 2.72E-5 23 4.98E-5
9 SGTC_996 3970-0799 3.52 1.8 µM 1.31E-4 112 2.15E-4
10 SGTC_304 1310-0298 3.37 97.0 µM 2.42E-4 cell wall 88 3.81E-4
11 SGTC_2690 usnic acid 3.36 100.0 µM 2.52E-4 53 3.96E-4
12 SGTC_769 0302-0231 3.33 76.0 µM 2.83E-4 82 4.41E-4
13 SGTC_737 1950-0117 3.30 57.9 µM 3.16E-4 42 4.87E-4
14 SGTC_1674 st016616 3.29 23.6 µM 3.22E-4 54 4.96E-4
15 SGTC_68 0986-0284 3.22 82.8 µM 4.18E-4 112 6.33E-4
16 SGTC_317 4495-0002 3.21 170.0 µM 4.37E-4 31 6.59E-4
17 SGTC_519 k048-0011 3.20 62.8 µM 4.65E-4 53 6.98E-4
18 SGTC_1177 0983-0204 3.18 23.6 µM 4.97E-4 37 7.43E-4
19 SGTC_2794 7785382 3.15 27.3 µM 5.41E-4 76 8.05E-4
20 SGTC_790 1575-0012 3.14 79.1 µM 5.68E-4 TSC3-RPN4 63 8.42E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.170 4.79E-23 YDR208W MSS4 het Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
0.167 1.85E-22 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.153 6.10E-19 YOR268C_p YOR268C_p hom Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene
0.152 7.81E-19 YNL268W LYP1 hom Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids
0.146 1.55E-17 YPR010C RPA135 het RNA polymerase I second largest subunit A135
0.136 2.97E-15 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.132 1.82E-14 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.128 1.25E-13 YGL061C DUO1 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
0.126 2.34E-13 YLR189C ATG26 hom UDP-glucose:sterol glucosyltransferase, conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy
0.123 9.70E-13 YAL045C_d YAL045C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A
0.118 7.38E-12 YKL001C MET14 hom Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism
0.117 1.31E-11 YNL011C_p YNL011C_p hom Putative protein of unknown function; YNL011C is not an essential gene
0.116 1.80E-11 YML119W_p YML119W_p hom Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate
0.113 5.12E-11 YDL121C_p YDL121C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein
0.113 5.40E-11 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)