YBR212W / NGR1

RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR212W / NGR1

Click on Significant Values for Screen Details ID:SGTC_95|Compound:0471-0001|FD-Score:-3.34|P-value:6.71E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:-3.23|P-value:9.43E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.83|P-value:1.16E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.56|P-value:3.55E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.32|P-value:7.91E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.40|P-value:6.06E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:3.53|P-value:3.96E-4 ID:SGTC_1207|Compound:2099-0101|FD-Score:3.37|P-value:6.73E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.29|P-value:7.88E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:-4.41|P-value:1.16E-5 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.83|P-value:1.33E-4 ID:SGTC_1547|Compound:harmine|FD-Score:3.54|P-value:3.77E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:-4.08|P-value:4.59E-5 ID:SGTC_1697|Compound:st025554|FD-Score:3.60|P-value:3.06E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.44|P-value:5.43E-4 ID:SGTC_1833|Compound:st055984|FD-Score:-3.58|P-value:2.96E-4 ID:SGTC_1923|Compound:st058458|FD-Score:3.36|P-value:6.99E-4 ID:SGTC_1927|Compound:st060202|FD-Score:3.80|P-value:1.51E-4 ID:SGTC_1934|Compound:st074720|FD-Score:-3.85|P-value:1.09E-4 ID:SGTC_1941|Compound:st076221|FD-Score:5.18|P-value:4.07E-7 ID:SGTC_2048|Compound:5246248|FD-Score:4.51|P-value:8.92E-6 ID:SGTC_2372|Compound:9071633|FD-Score:-4.28|P-value:1.98E-5 ID:SGTC_2380|Compound:9035439|FD-Score:3.44|P-value:5.46E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-3.51|P-value:3.77E-4 ID:SGTC_2476|Compound:5806916|FD-Score:4.44|P-value:1.20E-5 ID:SGTC_2478|Compound:5763493|FD-Score:-3.41|P-value:5.26E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.79|P-value:1.36E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:-3.42|P-value:5.12E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.51|P-value:3.71E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:6.11|P-value:2.85E-9 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.57|P-value:6.80E-6 ID:SGTC_2968|Compound:9090663|FD-Score:3.64|P-value:2.67E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.74|P-value:1.86E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:-3.34|P-value:6.71E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:-3.23|P-value:9.43E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.83|P-value:1.16E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.56|P-value:3.55E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.32|P-value:7.91E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.40|P-value:6.06E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:3.53|P-value:3.96E-4 ID:SGTC_1207|Compound:2099-0101|FD-Score:3.37|P-value:6.73E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.29|P-value:7.88E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:-4.41|P-value:1.16E-5 ID:SGTC_1416|Compound:3966-0321|FD-Score:3.83|P-value:1.33E-4 ID:SGTC_1547|Compound:harmine|FD-Score:3.54|P-value:3.77E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:-4.08|P-value:4.59E-5 ID:SGTC_1697|Compound:st025554|FD-Score:3.60|P-value:3.06E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.44|P-value:5.43E-4 ID:SGTC_1833|Compound:st055984|FD-Score:-3.58|P-value:2.96E-4 ID:SGTC_1923|Compound:st058458|FD-Score:3.36|P-value:6.99E-4 ID:SGTC_1927|Compound:st060202|FD-Score:3.80|P-value:1.51E-4 ID:SGTC_1934|Compound:st074720|FD-Score:-3.85|P-value:1.09E-4 ID:SGTC_1941|Compound:st076221|FD-Score:5.18|P-value:4.07E-7 ID:SGTC_2048|Compound:5246248|FD-Score:4.51|P-value:8.92E-6 ID:SGTC_2372|Compound:9071633|FD-Score:-4.28|P-value:1.98E-5 ID:SGTC_2380|Compound:9035439|FD-Score:3.44|P-value:5.46E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-3.51|P-value:3.77E-4 ID:SGTC_2476|Compound:5806916|FD-Score:4.44|P-value:1.20E-5 ID:SGTC_2478|Compound:5763493|FD-Score:-3.41|P-value:5.26E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.79|P-value:1.36E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:-3.42|P-value:5.12E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.51|P-value:3.71E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:6.11|P-value:2.85E-9 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.57|P-value:6.80E-6 ID:SGTC_2968|Compound:9090663|FD-Score:3.64|P-value:2.67E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.74|P-value:1.86E-4

Top fitness defect scores for YBR212W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2640 pentamidine 6.11 10.4 µM 2.85E-9 mitochondrial processes 5 4.86E-10
2 SGTC_1941 st076221 5.18 61.8 µM 4.07E-7 sphingolipid biosynthesis & PDR1 43 1.11E-7
3 SGTC_2651 1327-0036 4.57 5.0 µM 6.80E-6 130 2.44E-6
4 SGTC_2048 5246248 4.51 200.0 µM 8.92E-6 3 3.28E-6
5 SGTC_2476 5806916 4.44 10.5 µM 1.20E-5 8 4.52E-6
6 SGTC_1416 3966-0321 3.83 25.3 µM 1.33E-4 17 6.31E-5
7 SGTC_1927 st060202 3.80 13.8 µM 1.51E-4 22 7.27E-5
8 SGTC_3137 9097352 3.74 49.5 µM 1.86E-4 48 9.13E-5
9 SGTC_2968 9090663 3.64 33.4 µM 2.67E-4 45 1.35E-4
10 SGTC_1697 st025554 3.60 27.7 µM 3.06E-4 28 1.57E-4
11 SGTC_236 1509-0011 3.56 5.3 µM 3.55E-4 412 1.85E-4
12 SGTC_1547 harmine 3.54 94.2 µM 3.77E-4 ergosterol biosynthesis 61 1.98E-4
13 SGTC_939 1165-0479 3.53 45.3 µM 3.96E-4 49 2.08E-4
14 SGTC_1820 st053765 3.44 18.6 µM 5.43E-4 RPP1 & pyrimidine depletion 34 2.94E-4
15 SGTC_2380 9035439 3.44 200.0 µM 5.46E-4 endomembrane recycling 46 2.96E-4
16 SGTC_576 1073-0113 3.40 22.7 µM 6.06E-4 redox potentiating 86 3.32E-4
17 SGTC_1207 2099-0101 3.37 141.0 µM 6.73E-4 42 3.72E-4
18 SGTC_1923 st058458 3.36 19.1 µM 6.99E-4 58 3.88E-4
19 SGTC_568 1193-0097 3.32 7.5 µM 7.91E-4 ERAD & cell cycle 192 4.44E-4
20 SGTC_2754 granisetron 3.24 64.0 µM 0.00103 69 5.91E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.152 8.18E-19 YIL135C VHS2 hom Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p
0.137 1.29E-15 YOR297C TIM18 hom Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.120 2.64E-12 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.120 2.95E-12 YNL263C YIF1 het Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3
0.118 7.77E-12 YKL191W DPH2 hom Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
0.117 9.04E-12 YLR436C ECM30 hom Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm
0.117 9.86E-12 YOL013W-B_d YOL013W-B_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element
0.114 3.69E-11 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.113 5.67E-11 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.111 1.18E-10 YMR070W MOT3 hom Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion
0.110 2.01E-10 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.109 2.52E-10 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.108 3.77E-10 YIL069C RPS24B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication
0.107 4.36E-10 YBR068C BAP2 hom High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
0.107 5.54E-10 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit