HIPHOP chemogenomics database
YBR229C / ROT2 
Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
Zygosity: Homozygous strain
fixedexpanded
  Click on Significant Values for Screen Details |
Top fitness defect scores for YBR229C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.335 | 7.67E-89 | YNL322C | KRE1 | hom | Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor |
| 0.299 | 3.18E-70 | YGL027C | CWH41 | hom | Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress |
| 0.266 | 1.96E-55 | YOR085W | OST3 | hom | Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins |
| 0.244 | 1.08E-46 | YBR260C | RGD1 | hom | GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization |
| 0.242 | 8.72E-46 | YAL056C-A_d | YAL056C-A_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data |
| 0.236 | 1.12E-43 | YAL058W | CNE1 | hom | Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast |
| 0.228 | 1.17E-40 | YLR120C | YPS1 | hom | Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor |
| 0.225 | 7.46E-40 | YDL117W | CYK3 | hom | SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants |
| 0.220 | 3.43E-38 | YGR196C | FYV8 | hom | Protein of unknown function, required for survival upon exposure to K1 killer toxin |
| 0.217 | 4.66E-37 | YKL096C-B_p | YKL096C-B_p | hom | Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching |
| 0.212 | 2.63E-35 | YMR200W | ROT1 | het | Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation |
| 0.186 | 1.96E-27 | YDL096C_d | OPI6_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential |
| 0.179 | 1.58E-25 | YEL036C | ANP1 | hom | Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol |
| 0.177 | 4.55E-25 | YOR216C | RUD3 | hom | Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 |
| 0.177 | 6.00E-25 | YOR109W | INP53 | hom | Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains |