YBR243C / ALG7

UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR243C / ALG7

Click on Significant Values for Screen Details ID:SGTC_166|Compound:k072-0058|FD-Score:-3.92|P-value:5.92E-6 ID:SGTC_182|Compound:3448-4371|FD-Score:3.11|P-value:1.43E-4 ID:SGTC_186|Compound:3455-3465|FD-Score:2.66|P-value:9.29E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:35.20|P-value:0 ID:SGTC_372|Compound:0155-0182|FD-Score:2.77|P-value:6.05E-4 ID:SGTC_394|Compound:menadione|FD-Score:2.92|P-value:3.21E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:2.87|P-value:3.95E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-2.96|P-value:5.03E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:2.65|P-value:9.49E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.19|P-value:1.01E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:2.76|P-value:6.32E-4 ID:SGTC_761|Compound:k216-0224|FD-Score:2.74|P-value:6.74E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:2.66|P-value:9.16E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.16|P-value:2.14E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.80|P-value:5.26E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:2.93|P-value:3.15E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:-3.38|P-value:8.13E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:-2.80|P-value:9.32E-4 ID:SGTC_1209|Compound:2323-2347|FD-Score:3.36|P-value:4.59E-5 ID:SGTC_1231|Compound:0242-0792|FD-Score:-3.17|P-value:2.07E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:-2.98|P-value:4.70E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.26|P-value:1.40E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:-2.83|P-value:8.52E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:2.78|P-value:5.69E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.24|P-value:8.00E-5 ID:SGTC_1374|Compound:2889-5571|FD-Score:-2.81|P-value:8.95E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:-3.03|P-value:3.78E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.00|P-value:2.32E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.72|P-value:1.64E-5 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:2.89|P-value:3.64E-4 ID:SGTC_1725|Compound:st036284|FD-Score:3.39|P-value:3.90E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-3.65|P-value:2.27E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:21.80|P-value:7.95E-137 ID:SGTC_1811|Compound:st050069|FD-Score:3.31|P-value:5.71E-5 ID:SGTC_1815|Compound:st051655|FD-Score:2.67|P-value:8.97E-4 ID:SGTC_1862|Compound:st057513|FD-Score:-3.05|P-value:3.48E-4 ID:SGTC_1866|Compound:st057638|FD-Score:-4.57|P-value:1.47E-7 ID:SGTC_1871|Compound:st057448|FD-Score:-4.00|P-value:3.72E-6 ID:SGTC_1875|Compound:nocodazole|FD-Score:4.34|P-value:2.44E-7 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-2.85|P-value:7.61E-4 ID:SGTC_1932|Compound:st074806|FD-Score:-3.31|P-value:1.14E-4 ID:SGTC_2022|Compound:5136654|FD-Score:2.79|P-value:5.62E-4 ID:SGTC_2055|Compound:5235184|FD-Score:2.74|P-value:6.85E-4 ID:SGTC_2072|Compound:5231554|FD-Score:-2.97|P-value:4.85E-4 ID:SGTC_2109|Compound:5483026|FD-Score:2.77|P-value:5.99E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.27|P-value:7.09E-5 ID:SGTC_2134|Compound:5324836|FD-Score:-3.26|P-value:1.43E-4 ID:SGTC_2136|Compound:5328288|FD-Score:-4.17|P-value:1.51E-6 ID:SGTC_2141|Compound:5621638|FD-Score:-3.06|P-value:3.27E-4 ID:SGTC_2144|Compound:5628481|FD-Score:-2.85|P-value:7.88E-4 ID:SGTC_2158|Compound:5657123|FD-Score:5.34|P-value:3.36E-10 ID:SGTC_2336|Compound:9001819|FD-Score:3.06|P-value:1.77E-4 ID:SGTC_2339|Compound:9000433|FD-Score:-2.91|P-value:6.09E-4 ID:SGTC_2357|Compound:9067248|FD-Score:-3.80|P-value:1.09E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.32|P-value:6.45E-7 ID:SGTC_2413|Compound:inermin|FD-Score:23.40|P-value:7.15E-158 ID:SGTC_2428|Compound:5615643|FD-Score:4.15|P-value:7.30E-7 ID:SGTC_2467|Compound:5474540|FD-Score:-3.05|P-value:3.39E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.03|P-value:2.03E-4 ID:SGTC_2942|Compound:9052777|FD-Score:4.07|P-value:1.15E-6 ID:SGTC_2944|Compound:9067346|FD-Score:2.70|P-value:7.88E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-2.87|P-value:7.13E-4 ID:SGTC_3209|Compound:9113616|FD-Score:5.31|P-value:4.45E-10 ID:SGTC_3210|Compound:9128157|FD-Score:3.17|P-value:1.09E-4 ID:SGTC_3226|Compound:9131256|FD-Score:3.17|P-value:1.11E-4 ID:SGTC_3237|Compound:9133362|FD-Score:3.69|P-value:9.01E-6 ID:SGTC_3291|Compound:9121177|FD-Score:2.73|P-value:7.13E-4 ID:SGTC_3293|Compound:9120939|FD-Score:4.04|P-value:1.41E-6 ID:SGTC_3298|Compound:9116858|FD-Score:3.43|P-value:3.22E-5 ID:SGTC_3314|Compound:9135860|FD-Score:3.03|P-value:2.01E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.92|P-value:5.92E-6 ID:SGTC_182|Compound:3448-4371|FD-Score:3.11|P-value:1.43E-4 ID:SGTC_186|Compound:3455-3465|FD-Score:2.66|P-value:9.29E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:35.20|P-value:0 ID:SGTC_372|Compound:0155-0182|FD-Score:2.77|P-value:6.05E-4 ID:SGTC_394|Compound:menadione|FD-Score:2.92|P-value:3.21E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:2.87|P-value:3.95E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-2.96|P-value:5.03E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:2.65|P-value:9.49E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.19|P-value:1.01E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:2.76|P-value:6.32E-4 ID:SGTC_761|Compound:k216-0224|FD-Score:2.74|P-value:6.74E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:2.66|P-value:9.16E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.16|P-value:2.14E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.80|P-value:5.26E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:2.93|P-value:3.15E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:-3.38|P-value:8.13E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:-2.80|P-value:9.32E-4 ID:SGTC_1209|Compound:2323-2347|FD-Score:3.36|P-value:4.59E-5 ID:SGTC_1231|Compound:0242-0792|FD-Score:-3.17|P-value:2.07E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:-2.98|P-value:4.70E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.26|P-value:1.40E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:-2.83|P-value:8.52E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:2.78|P-value:5.69E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.24|P-value:8.00E-5 ID:SGTC_1374|Compound:2889-5571|FD-Score:-2.81|P-value:8.95E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:-3.03|P-value:3.78E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.00|P-value:2.32E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.72|P-value:1.64E-5 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:2.89|P-value:3.64E-4 ID:SGTC_1725|Compound:st036284|FD-Score:3.39|P-value:3.90E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-3.65|P-value:2.27E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:21.80|P-value:7.95E-137 ID:SGTC_1811|Compound:st050069|FD-Score:3.31|P-value:5.71E-5 ID:SGTC_1815|Compound:st051655|FD-Score:2.67|P-value:8.97E-4 ID:SGTC_1862|Compound:st057513|FD-Score:-3.05|P-value:3.48E-4 ID:SGTC_1866|Compound:st057638|FD-Score:-4.57|P-value:1.47E-7 ID:SGTC_1871|Compound:st057448|FD-Score:-4.00|P-value:3.72E-6 ID:SGTC_1875|Compound:nocodazole|FD-Score:4.34|P-value:2.44E-7 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-2.85|P-value:7.61E-4 ID:SGTC_1932|Compound:st074806|FD-Score:-3.31|P-value:1.14E-4 ID:SGTC_2022|Compound:5136654|FD-Score:2.79|P-value:5.62E-4 ID:SGTC_2055|Compound:5235184|FD-Score:2.74|P-value:6.85E-4 ID:SGTC_2072|Compound:5231554|FD-Score:-2.97|P-value:4.85E-4 ID:SGTC_2109|Compound:5483026|FD-Score:2.77|P-value:5.99E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.27|P-value:7.09E-5 ID:SGTC_2134|Compound:5324836|FD-Score:-3.26|P-value:1.43E-4 ID:SGTC_2136|Compound:5328288|FD-Score:-4.17|P-value:1.51E-6 ID:SGTC_2141|Compound:5621638|FD-Score:-3.06|P-value:3.27E-4 ID:SGTC_2144|Compound:5628481|FD-Score:-2.85|P-value:7.88E-4 ID:SGTC_2158|Compound:5657123|FD-Score:5.34|P-value:3.36E-10 ID:SGTC_2336|Compound:9001819|FD-Score:3.06|P-value:1.77E-4 ID:SGTC_2339|Compound:9000433|FD-Score:-2.91|P-value:6.09E-4 ID:SGTC_2357|Compound:9067248|FD-Score:-3.80|P-value:1.09E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.32|P-value:6.45E-7 ID:SGTC_2413|Compound:inermin|FD-Score:23.40|P-value:7.15E-158 ID:SGTC_2428|Compound:5615643|FD-Score:4.15|P-value:7.30E-7 ID:SGTC_2467|Compound:5474540|FD-Score:-3.05|P-value:3.39E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.03|P-value:2.03E-4 ID:SGTC_2942|Compound:9052777|FD-Score:4.07|P-value:1.15E-6 ID:SGTC_2944|Compound:9067346|FD-Score:2.70|P-value:7.88E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-2.87|P-value:7.13E-4 ID:SGTC_3209|Compound:9113616|FD-Score:5.31|P-value:4.45E-10 ID:SGTC_3210|Compound:9128157|FD-Score:3.17|P-value:1.09E-4 ID:SGTC_3226|Compound:9131256|FD-Score:3.17|P-value:1.11E-4 ID:SGTC_3237|Compound:9133362|FD-Score:3.69|P-value:9.01E-6 ID:SGTC_3291|Compound:9121177|FD-Score:2.73|P-value:7.13E-4 ID:SGTC_3293|Compound:9120939|FD-Score:4.04|P-value:1.41E-6 ID:SGTC_3298|Compound:9116858|FD-Score:3.43|P-value:3.22E-5 ID:SGTC_3314|Compound:9135860|FD-Score:3.03|P-value:2.01E-4

Top fitness defect scores for YBR243C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_255 tunicamycin 35.20 200.5 nM 0 unfolded protein response 1 3.05E-271 13.30
2 SGTC_2413 inermin 23.40 26.1 µM 7.15E-158 3 2.30E-121 5.39
3 SGTC_1789 tunicamycin 21.80 25.0 nM 7.95E-137 unfolded protein response 1 3.35E-105 6.26
4 SGTC_2158 5657123 5.34 3.6 µM 3.36E-10 6 4.54E-8 0.10
5 SGTC_3209 9113616 5.31 49.5 µM 4.45E-10 1 5.63E-8 0.67
6 SGTC_1875 nocodazole 4.34 6.0 µM 2.44E-7 tubulin folding & SWR complex 21 7.06E-6 0.18
7 SGTC_2428 5615643 4.15 7.8 µM 7.30E-7 6 1.63E-5 0.39
8 SGTC_2942 9052777 4.07 62.2 µM 1.15E-6 7 2.31E-5 0.18
9 SGTC_3293 9120939 4.04 62.0 µM 1.41E-6 9 2.71E-5 0.03
10 SGTC_3237 9133362 3.69 49.5 µM 9.01E-6 5 1.12E-4 0.25
11 SGTC_3298 9116858 3.43 71.4 µM 3.22E-5 8 2.97E-4 0.21
12 SGTC_1725 st036284 3.39 20.6 µM 3.90E-5 9 3.44E-4 0.09
13 SGTC_1209 2323-2347 3.36 50.3 µM 4.59E-5 6 3.90E-4 0.05
14 SGTC_1811 st050069 3.31 61.5 µM 5.71E-5 1 4.61E-4 0.17
15 SGTC_2130 NSC 7 3.27 2.5 µM 7.09E-5 copper-dependent oxidative stress 6 5.44E-4 0.13
16 SGTC_1350 1495-0454 3.24 146.0 µM 8.00E-5 9 5.97E-4 0.02
17 SGTC_724 3910-0327 3.19 137.0 µM 1.01E-4 ERG2 13 7.16E-4 0.01
18 SGTC_3210 9128157 3.17 49.5 µM 1.09E-4 25 7.57E-4 0.03
19 SGTC_3226 9131256 3.17 49.5 µM 1.11E-4 2 7.69E-4 0.28
20 SGTC_182 3448-4371 3.11 550.5 µM 1.43E-4 8 9.34E-4 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.430 3.84E-151 YNL287W SEC21 het Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
0.349 1.69E-96 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.331 2.84E-86 YKL104C GFA1 het Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
0.316 1.79E-78 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.310 1.11E-75 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.282 3.12E-62 YBL040C ERD2 het HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
0.272 8.74E-58 YDL145C COP1 het Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
0.270 5.28E-57 YFL031W HAC1 hom Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.235 2.60E-43 YDR238C SEC26 het Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
0.232 2.63E-42 YBR242W_p YBR242W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene
0.226 3.98E-40 YKL159C RCN1 hom Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region
0.213 6.99E-36 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.206 1.81E-33 YDR315C IPK1 hom Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
0.205 2.59E-33 YKL190W CNB1 hom Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress
0.200 1.67E-31 YDR190C RVB1 het ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly