YBR246W / RRT2

Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR246W / RRT2

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:3.79|P-value:2.34E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.33|P-value:6.57E-12 ID:SGTC_90|Compound:0870-0863|FD-Score:3.52|P-value:5.78E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:3.85|P-value:1.89E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:5.05|P-value:1.58E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:7.72|P-value:5.26E-13 ID:SGTC_169|Compound:k015-0017|FD-Score:5.05|P-value:1.58E-6 ID:SGTC_172|Compound:1112-0046|FD-Score:4.17|P-value:6.06E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:4.25|P-value:4.36E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:23.70|P-value:3.36E-106 ID:SGTC_232|Compound:clozapine|FD-Score:4.90|P-value:3.07E-6 ID:SGTC_301|Compound:4119-0100|FD-Score:5.06|P-value:1.52E-6 ID:SGTC_313|Compound:3474-0040|FD-Score:4.99|P-value:2.03E-6 ID:SGTC_345|Compound:1362-0200|FD-Score:3.40|P-value:8.64E-4 ID:SGTC_347|Compound:4534-1311|FD-Score:3.37|P-value:9.54E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:9.15|P-value:1.44E-17 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.52|P-value:5.82E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.56|P-value:5.09E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:6.53|P-value:8.48E-10 ID:SGTC_575|Compound:k072-0246|FD-Score:3.47|P-value:6.96E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:4.47|P-value:1.86E-5 ID:SGTC_705|Compound:1124-0212|FD-Score:-4.13|P-value:6.56E-5 ID:SGTC_706|Compound:4262-0057|FD-Score:4.31|P-value:3.47E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:5.70|P-value:7.16E-8 ID:SGTC_804|Compound:1595-0054|FD-Score:3.53|P-value:5.76E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:5.91|P-value:2.46E-8 ID:SGTC_1226|Compound:3909-7979|FD-Score:5.45|P-value:2.47E-7 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.58|P-value:4.87E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.66|P-value:3.64E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:4.14|P-value:6.82E-5 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:4.20|P-value:5.36E-5 ID:SGTC_1612|Compound:st002233|FD-Score:3.47|P-value:6.88E-4 ID:SGTC_1665|Compound:st014639|FD-Score:4.30|P-value:3.70E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.93|P-value:1.45E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.54|P-value:1.40E-5 ID:SGTC_1712|Compound:st032288|FD-Score:3.90|P-value:1.61E-4 ID:SGTC_1745|Compound:st081495|FD-Score:4.21|P-value:5.07E-5 ID:SGTC_1754|Compound:st042065|FD-Score:9.33|P-value:3.49E-18 ID:SGTC_1760|Compound:st045453|FD-Score:10.00|P-value:1.27E-20 ID:SGTC_1800|Compound:st050079|FD-Score:6.75|P-value:2.38E-10 ID:SGTC_1801|Compound:st050089|FD-Score:10.30|P-value:1.26E-21 ID:SGTC_1803|Compound:st050068|FD-Score:11.70|P-value:1.33E-27 ID:SGTC_1818|Compound:st052136|FD-Score:5.11|P-value:1.19E-6 ID:SGTC_1934|Compound:st074720|FD-Score:4.19|P-value:5.60E-5 ID:SGTC_1941|Compound:st076221|FD-Score:5.28|P-value:5.51E-7 ID:SGTC_1954|Compound:st077032|FD-Score:-4.02|P-value:9.74E-5 ID:SGTC_1991|Compound:st073000|FD-Score:4.88|P-value:3.31E-6 ID:SGTC_2014|Compound:4013930|FD-Score:-5.83|P-value:3.33E-8 ID:SGTC_2020|Compound:4017018|FD-Score:4.71|P-value:7.09E-6 ID:SGTC_2021|Compound:4019055|FD-Score:4.34|P-value:3.07E-5 ID:SGTC_2024|Compound:5144227|FD-Score:-4.54|P-value:1.29E-5 ID:SGTC_2082|Compound:5219693|FD-Score:4.18|P-value:5.73E-5 ID:SGTC_2083|Compound:5306046|FD-Score:5.33|P-value:4.40E-7 ID:SGTC_2105|Compound:5350118|FD-Score:7.17|P-value:1.82E-11 ID:SGTC_2119|Compound:5528445|FD-Score:3.49|P-value:6.54E-4 ID:SGTC_2184|Compound:6075246|FD-Score:3.48|P-value:6.61E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.79|P-value:4.94E-6 ID:SGTC_2345|Compound:9052315|FD-Score:3.42|P-value:8.14E-4 ID:SGTC_2388|Compound:9080168|FD-Score:3.46|P-value:7.03E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.66|P-value:3.64E-4 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:-3.81|P-value:2.08E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.37|P-value:9.31E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.26|P-value:6.16E-7 ID:SGTC_2732|Compound:terfenadine|FD-Score:3.56|P-value:5.06E-4 ID:SGTC_2735|Compound:sertaconazole|FD-Score:4.99|P-value:2.08E-6 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.66|P-value:3.69E-4 ID:SGTC_2834|Compound:9001755|FD-Score:4.78|P-value:5.18E-6 ID:SGTC_2858|Compound:9025781|FD-Score:6.34|P-value:2.38E-9 ID:SGTC_2896|Compound:9052656|FD-Score:8.28|P-value:1.04E-14 ID:SGTC_2950|Compound:9072895|FD-Score:-3.37|P-value:9.00E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.24|P-value:4.58E-5 ID:SGTC_3045|Compound:9092950|FD-Score:-3.92|P-value:1.40E-4 ID:SGTC_3056|Compound:9094900|FD-Score:4.20|P-value:5.26E-5 ID:SGTC_3087|Compound:9117406|FD-Score:-5.25|P-value:5.74E-7 ID:SGTC_3098|Compound:9119039|FD-Score:5.76|P-value:5.18E-8 ID:SGTC_3148|Compound:9097893|FD-Score:9.12|P-value:1.80E-17 ID:SGTC_3152|Compound:9099253|FD-Score:13.60|P-value:1.28E-36 ID:SGTC_3155|Compound:9099265|FD-Score:-3.56|P-value:4.90E-4 ID:SGTC_3185|Compound:9108267|FD-Score:4.11|P-value:7.54E-5 ID:SGTC_3218|Compound:9131285|FD-Score:-4.03|P-value:9.58E-5 ID:SGTC_3300|Compound:9124615|FD-Score:-3.58|P-value:4.54E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.79|P-value:2.34E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.33|P-value:6.57E-12 ID:SGTC_90|Compound:0870-0863|FD-Score:3.52|P-value:5.78E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:3.85|P-value:1.89E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:5.05|P-value:1.58E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:7.72|P-value:5.26E-13 ID:SGTC_169|Compound:k015-0017|FD-Score:5.05|P-value:1.58E-6 ID:SGTC_172|Compound:1112-0046|FD-Score:4.17|P-value:6.06E-5 ID:SGTC_178|Compound:4092-0806|FD-Score:4.25|P-value:4.36E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:23.70|P-value:3.36E-106 ID:SGTC_232|Compound:clozapine|FD-Score:4.90|P-value:3.07E-6 ID:SGTC_301|Compound:4119-0100|FD-Score:5.06|P-value:1.52E-6 ID:SGTC_313|Compound:3474-0040|FD-Score:4.99|P-value:2.03E-6 ID:SGTC_345|Compound:1362-0200|FD-Score:3.40|P-value:8.64E-4 ID:SGTC_347|Compound:4534-1311|FD-Score:3.37|P-value:9.54E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:9.15|P-value:1.44E-17 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.52|P-value:5.82E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.56|P-value:5.09E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:6.53|P-value:8.48E-10 ID:SGTC_575|Compound:k072-0246|FD-Score:3.47|P-value:6.96E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:4.47|P-value:1.86E-5 ID:SGTC_705|Compound:1124-0212|FD-Score:-4.13|P-value:6.56E-5 ID:SGTC_706|Compound:4262-0057|FD-Score:4.31|P-value:3.47E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:5.70|P-value:7.16E-8 ID:SGTC_804|Compound:1595-0054|FD-Score:3.53|P-value:5.76E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:5.91|P-value:2.46E-8 ID:SGTC_1226|Compound:3909-7979|FD-Score:5.45|P-value:2.47E-7 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.58|P-value:4.87E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.66|P-value:3.64E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:4.14|P-value:6.82E-5 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:4.20|P-value:5.36E-5 ID:SGTC_1612|Compound:st002233|FD-Score:3.47|P-value:6.88E-4 ID:SGTC_1665|Compound:st014639|FD-Score:4.30|P-value:3.70E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.93|P-value:1.45E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.54|P-value:1.40E-5 ID:SGTC_1712|Compound:st032288|FD-Score:3.90|P-value:1.61E-4 ID:SGTC_1745|Compound:st081495|FD-Score:4.21|P-value:5.07E-5 ID:SGTC_1754|Compound:st042065|FD-Score:9.33|P-value:3.49E-18 ID:SGTC_1760|Compound:st045453|FD-Score:10.00|P-value:1.27E-20 ID:SGTC_1800|Compound:st050079|FD-Score:6.75|P-value:2.38E-10 ID:SGTC_1801|Compound:st050089|FD-Score:10.30|P-value:1.26E-21 ID:SGTC_1803|Compound:st050068|FD-Score:11.70|P-value:1.33E-27 ID:SGTC_1818|Compound:st052136|FD-Score:5.11|P-value:1.19E-6 ID:SGTC_1934|Compound:st074720|FD-Score:4.19|P-value:5.60E-5 ID:SGTC_1941|Compound:st076221|FD-Score:5.28|P-value:5.51E-7 ID:SGTC_1954|Compound:st077032|FD-Score:-4.02|P-value:9.74E-5 ID:SGTC_1991|Compound:st073000|FD-Score:4.88|P-value:3.31E-6 ID:SGTC_2014|Compound:4013930|FD-Score:-5.83|P-value:3.33E-8 ID:SGTC_2020|Compound:4017018|FD-Score:4.71|P-value:7.09E-6 ID:SGTC_2021|Compound:4019055|FD-Score:4.34|P-value:3.07E-5 ID:SGTC_2024|Compound:5144227|FD-Score:-4.54|P-value:1.29E-5 ID:SGTC_2082|Compound:5219693|FD-Score:4.18|P-value:5.73E-5 ID:SGTC_2083|Compound:5306046|FD-Score:5.33|P-value:4.40E-7 ID:SGTC_2105|Compound:5350118|FD-Score:7.17|P-value:1.82E-11 ID:SGTC_2119|Compound:5528445|FD-Score:3.49|P-value:6.54E-4 ID:SGTC_2184|Compound:6075246|FD-Score:3.48|P-value:6.61E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.79|P-value:4.94E-6 ID:SGTC_2345|Compound:9052315|FD-Score:3.42|P-value:8.14E-4 ID:SGTC_2388|Compound:9080168|FD-Score:3.46|P-value:7.03E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.66|P-value:3.64E-4 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:-3.81|P-value:2.08E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.37|P-value:9.31E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.26|P-value:6.16E-7 ID:SGTC_2732|Compound:terfenadine|FD-Score:3.56|P-value:5.06E-4 ID:SGTC_2735|Compound:sertaconazole|FD-Score:4.99|P-value:2.08E-6 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.66|P-value:3.69E-4 ID:SGTC_2834|Compound:9001755|FD-Score:4.78|P-value:5.18E-6 ID:SGTC_2858|Compound:9025781|FD-Score:6.34|P-value:2.38E-9 ID:SGTC_2896|Compound:9052656|FD-Score:8.28|P-value:1.04E-14 ID:SGTC_2950|Compound:9072895|FD-Score:-3.37|P-value:9.00E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.24|P-value:4.58E-5 ID:SGTC_3045|Compound:9092950|FD-Score:-3.92|P-value:1.40E-4 ID:SGTC_3056|Compound:9094900|FD-Score:4.20|P-value:5.26E-5 ID:SGTC_3087|Compound:9117406|FD-Score:-5.25|P-value:5.74E-7 ID:SGTC_3098|Compound:9119039|FD-Score:5.76|P-value:5.18E-8 ID:SGTC_3148|Compound:9097893|FD-Score:9.12|P-value:1.80E-17 ID:SGTC_3152|Compound:9099253|FD-Score:13.60|P-value:1.28E-36 ID:SGTC_3155|Compound:9099265|FD-Score:-3.56|P-value:4.90E-4 ID:SGTC_3185|Compound:9108267|FD-Score:4.11|P-value:7.54E-5 ID:SGTC_3218|Compound:9131285|FD-Score:-4.03|P-value:9.58E-5 ID:SGTC_3300|Compound:9124615|FD-Score:-3.58|P-value:4.54E-4

Top fitness defect scores for YBR246W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_221 trifluoperazine 23.70 10.3 µM 3.36E-106 NEO1-PIK1 7 5.13E-124
2 SGTC_3152 9099253 13.60 49.5 µM 1.28E-36 NEO1-PIK1 7 1.44E-42
3 SGTC_1803 st050068 11.70 52.4 µM 1.33E-27 NEO1-PIK1 15 5.10E-32
4 SGTC_1801 st050089 10.30 28.4 µM 1.26E-21 NEO1-PIK1 13 5.01E-25
5 SGTC_1760 st045453 10.00 10.4 µM 1.27E-20 NEO1-PIK1 34 7.50E-24
6 SGTC_1754 st042065 9.33 16.1 µM 3.49E-18 NEO1-PIK1 35 5.33E-21
7 SGTC_439 amiodarone 9.15 34.4 µM 1.44E-17 NEO1-PIK1 16 2.79E-20
8 SGTC_3148 9097893 9.12 49.5 µM 1.80E-17 2 3.62E-20
9 SGTC_2896 9052656 8.28 71.4 µM 1.04E-14 endomembrane recycling 15 6.17E-17
10 SGTC_163 1327-0036 7.72 7.5 µM 5.26E-13 21 6.02E-15
11 SGTC_58 0957-0393 7.33 9.0 µM 6.57E-12 cell wall 152 1.15E-13
12 SGTC_2105 5350118 7.17 200.0 µM 1.82E-11 4 3.80E-13
13 SGTC_1800 st050079 6.75 29.1 µM 2.38E-10 NEO1-PIK1 30 7.65E-12
14 SGTC_528 3492-0019 6.53 172.0 µM 8.48E-10 calcium & mitochondrial duress 107 3.37E-11
15 SGTC_2858 9025781 6.34 9.0 µM 2.38E-9 sphingolipid biosynthesis & PDR1 41 1.12E-10
16 SGTC_1083 didemethylchlorpromazine sulfoxide 5.91 707.4 nM 2.46E-8 redox potentiating 28 1.72E-9
17 SGTC_3098 9119039 5.76 49.5 µM 5.18E-8 Golgi 6 4.10E-9
18 SGTC_801 0866-0028 5.70 10.1 µM 7.16E-8 6 5.98E-9
19 SGTC_1226 3909-7979 5.45 35.4 µM 2.47E-7 16 2.53E-8
20 SGTC_2083 5306046 5.33 65.9 µM 4.40E-7 3 4.96E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.473 1.06E-185 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.454 2.33E-170 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.447 7.64E-165 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.445 3.45E-163 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.434 6.25E-154 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.432 1.01E-152 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.413 1.98E-138 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.412 1.06E-137 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.398 1.99E-127 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.397 5.44E-127 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.397 9.52E-127 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.393 1.23E-124 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.383 2.05E-117 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.379 3.93E-115 YLR057W MNL2 hom Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
0.340 8.69E-92 YKL191W DPH2 hom Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p