YBR249C / ARO4

3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR249C / ARO4

Click on Significant Values for Screen Details ID:SGTC_46|Compound:0329-0187a|FD-Score:3.31|P-value:3.89E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.08|P-value:1.21E-7 ID:SGTC_87|Compound:0100-0052|FD-Score:3.73|P-value:7.36E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:4.45|P-value:3.07E-6 ID:SGTC_169|Compound:k015-0017|FD-Score:3.39|P-value:2.90E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:3.05|P-value:9.73E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:4.50|P-value:2.45E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.78|P-value:5.96E-7 ID:SGTC_314|Compound:1671-0008|FD-Score:6.57|P-value:1.25E-11 ID:SGTC_669|Compound:0108-0021|FD-Score:4.07|P-value:1.75E-5 ID:SGTC_927|Compound:2425-1185|FD-Score:-3.11|P-value:7.88E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.22|P-value:5.32E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:-3.20|P-value:5.81E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:-4.49|P-value:2.57E-6 ID:SGTC_1051|Compound:3253-3903|FD-Score:-3.72|P-value:7.73E-5 ID:SGTC_1059|Compound:3448-6867|FD-Score:4.53|P-value:2.07E-6 ID:SGTC_1226|Compound:3909-7979|FD-Score:3.92|P-value:3.40E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.07|P-value:9.12E-4 ID:SGTC_1481|Compound:3346-2004|FD-Score:-3.55|P-value:1.53E-4 ID:SGTC_1496|Compound:4469-0713|FD-Score:3.78|P-value:6.08E-5 ID:SGTC_1498|Compound:4478-3555|FD-Score:3.62|P-value:1.18E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-5.78|P-value:2.22E-9 ID:SGTC_1527|Compound:coumarin|FD-Score:-3.45|P-value:2.29E-4 ID:SGTC_1848|Compound:st056003|FD-Score:-3.09|P-value:8.38E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.41|P-value:2.62E-4 ID:SGTC_1944|Compound:st076371|FD-Score:4.17|P-value:1.12E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:4.47|P-value:2.82E-6 ID:SGTC_2012|Compound:4023501|FD-Score:-3.64|P-value:1.08E-4 ID:SGTC_2132|Compound:5317594|FD-Score:3.43|P-value:2.49E-4 ID:SGTC_2327|Compound:6189656|FD-Score:3.29|P-value:4.19E-4 ID:SGTC_2411|Compound:st077133|FD-Score:4.04|P-value:2.04E-5 ID:SGTC_2460|Compound:5552655|FD-Score:-3.17|P-value:6.53E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:5.25|P-value:4.87E-8 ID:SGTC_2569|Compound:tetrandrine|FD-Score:4.23|P-value:8.69E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.20|P-value:5.71E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:-3.15|P-value:6.84E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:3.29|P-value:4.15E-4 ID:SGTC_2830|Compound:7992007|FD-Score:3.41|P-value:2.64E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-3.10|P-value:8.19E-4 ID:SGTC_3014|Compound:9081533|FD-Score:-3.27|P-value:4.45E-4 ID:SGTC_3016|Compound:9080866|FD-Score:-3.35|P-value:3.30E-4 ID:SGTC_3351|Compound:9150312|FD-Score:-3.53|P-value:1.71E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.31|P-value:3.89E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.08|P-value:1.21E-7 ID:SGTC_87|Compound:0100-0052|FD-Score:3.73|P-value:7.36E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:4.45|P-value:3.07E-6 ID:SGTC_169|Compound:k015-0017|FD-Score:3.39|P-value:2.90E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:3.05|P-value:9.73E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:4.50|P-value:2.45E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.78|P-value:5.96E-7 ID:SGTC_314|Compound:1671-0008|FD-Score:6.57|P-value:1.25E-11 ID:SGTC_669|Compound:0108-0021|FD-Score:4.07|P-value:1.75E-5 ID:SGTC_927|Compound:2425-1185|FD-Score:-3.11|P-value:7.88E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.22|P-value:5.32E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:-3.20|P-value:5.81E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:-4.49|P-value:2.57E-6 ID:SGTC_1051|Compound:3253-3903|FD-Score:-3.72|P-value:7.73E-5 ID:SGTC_1059|Compound:3448-6867|FD-Score:4.53|P-value:2.07E-6 ID:SGTC_1226|Compound:3909-7979|FD-Score:3.92|P-value:3.40E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.07|P-value:9.12E-4 ID:SGTC_1481|Compound:3346-2004|FD-Score:-3.55|P-value:1.53E-4 ID:SGTC_1496|Compound:4469-0713|FD-Score:3.78|P-value:6.08E-5 ID:SGTC_1498|Compound:4478-3555|FD-Score:3.62|P-value:1.18E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-5.78|P-value:2.22E-9 ID:SGTC_1527|Compound:coumarin|FD-Score:-3.45|P-value:2.29E-4 ID:SGTC_1848|Compound:st056003|FD-Score:-3.09|P-value:8.38E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.41|P-value:2.62E-4 ID:SGTC_1944|Compound:st076371|FD-Score:4.17|P-value:1.12E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:4.47|P-value:2.82E-6 ID:SGTC_2012|Compound:4023501|FD-Score:-3.64|P-value:1.08E-4 ID:SGTC_2132|Compound:5317594|FD-Score:3.43|P-value:2.49E-4 ID:SGTC_2327|Compound:6189656|FD-Score:3.29|P-value:4.19E-4 ID:SGTC_2411|Compound:st077133|FD-Score:4.04|P-value:2.04E-5 ID:SGTC_2460|Compound:5552655|FD-Score:-3.17|P-value:6.53E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:5.25|P-value:4.87E-8 ID:SGTC_2569|Compound:tetrandrine|FD-Score:4.23|P-value:8.69E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.20|P-value:5.71E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:-3.15|P-value:6.84E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:3.29|P-value:4.15E-4 ID:SGTC_2830|Compound:7992007|FD-Score:3.41|P-value:2.64E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-3.10|P-value:8.19E-4 ID:SGTC_3014|Compound:9081533|FD-Score:-3.27|P-value:4.45E-4 ID:SGTC_3016|Compound:9080866|FD-Score:-3.35|P-value:3.30E-4 ID:SGTC_3351|Compound:9150312|FD-Score:-3.53|P-value:1.71E-4

Top fitness defect scores for YBR249C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_314 1671-0008 6.57 2.7 µM 1.25E-11 3 2.57E-11
2 SGTC_2568 diosmetin 5.25 100.0 µM 4.87E-8 5 7.76E-8
3 SGTC_1059 3448-6867 4.53 171.0 µM 2.07E-6 4 2.95E-6
4 SGTC_305 1682-2595 4.50 20.8 µM 2.45E-6 RNA processing & uracil transport 15 3.46E-6
5 SGTC_1955 wogonin 4.47 32.4 µM 2.82E-6 12 3.97E-6
6 SGTC_163 1327-0036 4.45 7.5 µM 3.07E-6 84 4.31E-6
7 SGTC_2569 tetrandrine 4.23 100.0 µM 8.69E-6 17 1.18E-5
8 SGTC_1944 st076371 4.17 56.9 µM 1.12E-5 17 1.52E-5
9 SGTC_669 0108-0021 4.07 26.2 µM 1.75E-5 fatty acid desaturase (OLE1) 74 2.33E-5
10 SGTC_2411 st077133 4.04 39.9 µM 2.04E-5 RNA pol III & RNase P/MRP 30 2.70E-5
11 SGTC_1226 3909-7979 3.92 35.4 µM 3.40E-5 39 4.44E-5
12 SGTC_1496 4469-0713 3.78 57.4 µM 6.08E-5 25 7.81E-5
13 SGTC_87 0100-0052 3.73 35.5 µM 7.36E-5 125 9.39E-5
14 SGTC_1498 4478-3555 3.62 97.6 µM 1.18E-4 7 1.48E-4
15 SGTC_2132 5317594 3.43 122.7 µM 2.49E-4 SWF1 & branched chain AA biosynthesis 38 3.06E-4
16 SGTC_1907 st060210 3.41 83.7 µM 2.62E-4 39 3.22E-4
17 SGTC_2830 7992007 3.41 71.4 µM 2.64E-4 38 3.24E-4
18 SGTC_169 k015-0017 3.39 16.0 µM 2.90E-4 34 3.55E-4
19 SGTC_46 0329-0187a 3.31 2.2 µM 3.89E-4 103 4.72E-4
20 SGTC_2587 piperic acid 3.29 92.2 µM 4.15E-4 43 5.03E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.154 2.97E-19 YOR274W MOD5 hom Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis
0.144 4.20E-17 YBR068C BAP2 hom High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
0.143 7.01E-17 YDR156W RPA14 hom RNA polymerase I subunit A14
0.142 1.23E-16 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.127 1.89E-13 YDR067C_p OCA6_p hom Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT
0.122 1.55E-12 YDR510W SMT3 het Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
0.121 2.23E-12 YHR025W THR1 hom Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.119 5.21E-12 YBR162W-A YSY6 hom Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion
0.116 1.77E-11 YNL067W-B_p YNL067W-B_p hom Putative protein of unknown function
0.116 1.93E-11 YGR083C GCD2 het Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.115 2.64E-11 YNL056W OCA2 hom Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene
0.115 2.91E-11 YGR159C NSR1 hom Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.112 9.63E-11 YDR277C MTH1 hom Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation
0.111 1.30E-10 YLR367W RPS22B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
0.107 4.58E-10 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W