YBR255W / MTC4

Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR255W / MTC4

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:5.56|P-value:2.49E-7 ID:SGTC_13|Compound:0199-0087|FD-Score:3.43|P-value:9.54E-4 ID:SGTC_14|Compound:0354-0009|FD-Score:3.60|P-value:5.75E-4 ID:SGTC_17|Compound:0960-0276|FD-Score:5.20|P-value:1.25E-6 ID:SGTC_31|Compound:0508-1974|FD-Score:3.97|P-value:1.67E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:13.50|P-value:1.33E-34 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.73|P-value:3.49E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:6.99|P-value:1.28E-10 ID:SGTC_61|Compound:0978-8327|FD-Score:6.99|P-value:1.24E-10 ID:SGTC_87|Compound:0100-0052|FD-Score:5.66|P-value:1.52E-7 ID:SGTC_91|Compound:0417-1751|FD-Score:4.19|P-value:7.51E-5 ID:SGTC_110|Compound:0850-0284|FD-Score:3.71|P-value:4.01E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:6.69|P-value:7.00E-10 ID:SGTC_161|Compound:4065-0411|FD-Score:5.83|P-value:6.47E-8 ID:SGTC_217|Compound:ketoconazole|FD-Score:-4.55|P-value:1.82E-5 ID:SGTC_253|Compound:dyclonine|FD-Score:3.58|P-value:6.14E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.44|P-value:9.34E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:3.69|P-value:4.22E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:4.86|P-value:5.50E-6 ID:SGTC_389|Compound:tpck|FD-Score:3.42|P-value:9.82E-4 ID:SGTC_409|Compound:arachidonic acid|FD-Score:3.70|P-value:4.10E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:4.07|P-value:1.15E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:11.40|P-value:3.88E-25 ID:SGTC_637|Compound:1181-0519|FD-Score:7.16|P-value:4.68E-11 ID:SGTC_645|Compound:0845-0943|FD-Score:4.39|P-value:3.64E-5 ID:SGTC_855|Compound:0428-0020|FD-Score:3.55|P-value:6.58E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:3.52|P-value:7.32E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:3.87|P-value:2.30E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.43|P-value:9.69E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:3.49|P-value:7.98E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:-4.59|P-value:1.54E-5 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.83|P-value:2.69E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.05|P-value:1.27E-4 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.58|P-value:6.12E-4 ID:SGTC_1448|Compound:4255-0083|FD-Score:4.33|P-value:4.43E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:3.95|P-value:1.75E-4 ID:SGTC_1670|Compound:st016595|FD-Score:3.43|P-value:9.54E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.62|P-value:5.40E-4 ID:SGTC_1715|Compound:st032160|FD-Score:-6.15|P-value:1.20E-8 ID:SGTC_1716|Compound:st033144|FD-Score:-7.46|P-value:6.48E-12 ID:SGTC_1721|Compound:st082152|FD-Score:4.30|P-value:4.99E-5 ID:SGTC_1750|Compound:st081859|FD-Score:20.40|P-value:1.25E-76 ID:SGTC_1814|Compound:st052110|FD-Score:-4.07|P-value:1.08E-4 ID:SGTC_1948|Compound:st076329|FD-Score:-3.55|P-value:6.25E-4 ID:SGTC_2020|Compound:4017018|FD-Score:3.95|P-value:1.76E-4 ID:SGTC_2026|Compound:5148700|FD-Score:4.26|P-value:5.77E-5 ID:SGTC_2159|Compound:5658611|FD-Score:-3.46|P-value:8.33E-4 ID:SGTC_2334|Compound:9017922|FD-Score:3.71|P-value:4.02E-4 ID:SGTC_2401|Compound:5128683|FD-Score:5.74|P-value:1.01E-7 ID:SGTC_2544|Compound:sphondin|FD-Score:-3.97|P-value:1.54E-4 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:-3.78|P-value:3.01E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.52|P-value:7.02E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-4.31|P-value:4.60E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.50|P-value:7.81E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.86|P-value:2.41E-4 ID:SGTC_2841|Compound:9004761|FD-Score:5.37|P-value:5.80E-7 ID:SGTC_2855|Compound:9016525|FD-Score:5.16|P-value:1.50E-6 ID:SGTC_2858|Compound:9025781|FD-Score:7.35|P-value:1.46E-11 ID:SGTC_2930|Compound:9013230|FD-Score:3.46|P-value:8.74E-4 ID:SGTC_2944|Compound:9067346|FD-Score:3.46|P-value:8.90E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.60|P-value:5.75E-4 ID:SGTC_3152|Compound:9099253|FD-Score:12.70|P-value:5.59E-31 ID:SGTC_3154|Compound:9100113|FD-Score:-4.16|P-value:7.94E-5 ID:SGTC_3214|Compound:9129694|FD-Score:4.30|P-value:5.11E-5 ID:SGTC_3244|Compound:9134489|FD-Score:-3.58|P-value:5.83E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.47|P-value:8.63E-4 ID:SGTC_3348|Compound:9149464|FD-Score:4.38|P-value:3.70E-5 ID:SGTC_8|Compound:0354-0001|FD-Score:5.56|P-value:2.49E-7 ID:SGTC_13|Compound:0199-0087|FD-Score:3.43|P-value:9.54E-4 ID:SGTC_14|Compound:0354-0009|FD-Score:3.60|P-value:5.75E-4 ID:SGTC_17|Compound:0960-0276|FD-Score:5.20|P-value:1.25E-6 ID:SGTC_31|Compound:0508-1974|FD-Score:3.97|P-value:1.67E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:13.50|P-value:1.33E-34 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.73|P-value:3.49E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:6.99|P-value:1.28E-10 ID:SGTC_61|Compound:0978-8327|FD-Score:6.99|P-value:1.24E-10 ID:SGTC_87|Compound:0100-0052|FD-Score:5.66|P-value:1.52E-7 ID:SGTC_91|Compound:0417-1751|FD-Score:4.19|P-value:7.51E-5 ID:SGTC_110|Compound:0850-0284|FD-Score:3.71|P-value:4.01E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:6.69|P-value:7.00E-10 ID:SGTC_161|Compound:4065-0411|FD-Score:5.83|P-value:6.47E-8 ID:SGTC_217|Compound:ketoconazole|FD-Score:-4.55|P-value:1.82E-5 ID:SGTC_253|Compound:dyclonine|FD-Score:3.58|P-value:6.14E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.44|P-value:9.34E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:3.69|P-value:4.22E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:4.86|P-value:5.50E-6 ID:SGTC_389|Compound:tpck|FD-Score:3.42|P-value:9.82E-4 ID:SGTC_409|Compound:arachidonic acid|FD-Score:3.70|P-value:4.10E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:4.07|P-value:1.15E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:11.40|P-value:3.88E-25 ID:SGTC_637|Compound:1181-0519|FD-Score:7.16|P-value:4.68E-11 ID:SGTC_645|Compound:0845-0943|FD-Score:4.39|P-value:3.64E-5 ID:SGTC_855|Compound:0428-0020|FD-Score:3.55|P-value:6.58E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:3.52|P-value:7.32E-4 ID:SGTC_1017|Compound:4239-0030|FD-Score:3.87|P-value:2.30E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.43|P-value:9.69E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:3.49|P-value:7.98E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:-4.59|P-value:1.54E-5 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.83|P-value:2.69E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.05|P-value:1.27E-4 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.58|P-value:6.12E-4 ID:SGTC_1448|Compound:4255-0083|FD-Score:4.33|P-value:4.43E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:3.95|P-value:1.75E-4 ID:SGTC_1670|Compound:st016595|FD-Score:3.43|P-value:9.54E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.62|P-value:5.40E-4 ID:SGTC_1715|Compound:st032160|FD-Score:-6.15|P-value:1.20E-8 ID:SGTC_1716|Compound:st033144|FD-Score:-7.46|P-value:6.48E-12 ID:SGTC_1721|Compound:st082152|FD-Score:4.30|P-value:4.99E-5 ID:SGTC_1750|Compound:st081859|FD-Score:20.40|P-value:1.25E-76 ID:SGTC_1814|Compound:st052110|FD-Score:-4.07|P-value:1.08E-4 ID:SGTC_1948|Compound:st076329|FD-Score:-3.55|P-value:6.25E-4 ID:SGTC_2020|Compound:4017018|FD-Score:3.95|P-value:1.76E-4 ID:SGTC_2026|Compound:5148700|FD-Score:4.26|P-value:5.77E-5 ID:SGTC_2159|Compound:5658611|FD-Score:-3.46|P-value:8.33E-4 ID:SGTC_2334|Compound:9017922|FD-Score:3.71|P-value:4.02E-4 ID:SGTC_2401|Compound:5128683|FD-Score:5.74|P-value:1.01E-7 ID:SGTC_2544|Compound:sphondin|FD-Score:-3.97|P-value:1.54E-4 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:-3.78|P-value:3.01E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.52|P-value:7.02E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-4.31|P-value:4.60E-5 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.50|P-value:7.81E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.86|P-value:2.41E-4 ID:SGTC_2841|Compound:9004761|FD-Score:5.37|P-value:5.80E-7 ID:SGTC_2855|Compound:9016525|FD-Score:5.16|P-value:1.50E-6 ID:SGTC_2858|Compound:9025781|FD-Score:7.35|P-value:1.46E-11 ID:SGTC_2930|Compound:9013230|FD-Score:3.46|P-value:8.74E-4 ID:SGTC_2944|Compound:9067346|FD-Score:3.46|P-value:8.90E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.60|P-value:5.75E-4 ID:SGTC_3152|Compound:9099253|FD-Score:12.70|P-value:5.59E-31 ID:SGTC_3154|Compound:9100113|FD-Score:-4.16|P-value:7.94E-5 ID:SGTC_3214|Compound:9129694|FD-Score:4.30|P-value:5.11E-5 ID:SGTC_3244|Compound:9134489|FD-Score:-3.58|P-value:5.83E-4 ID:SGTC_3316|Compound:9136639|FD-Score:3.47|P-value:8.63E-4 ID:SGTC_3348|Compound:9149464|FD-Score:4.38|P-value:3.70E-5

Top fitness defect scores for YBR255W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1750 st081859 20.40 2.4 µM 1.25E-76 endomembrane recycling 13 6.47E-93
2 SGTC_56 0957-0365 13.50 83.2 µM 1.33E-34 Golgi 2 9.57E-42
3 SGTC_3152 9099253 12.70 49.5 µM 5.59E-31 NEO1-PIK1 11 2.45E-37
4 SGTC_568 1193-0097 11.40 7.5 µM 3.88E-25 ERAD & cell cycle 14 3.15E-30
5 SGTC_2858 9025781 7.35 9.0 µM 1.46E-11 sphingolipid biosynthesis & PDR1 34 1.01E-13
6 SGTC_637 1181-0519 7.16 7.3 µM 4.68E-11 fatty acid desaturase (OLE1) 39 4.16E-13
7 SGTC_61 0978-8327 6.99 98.6 µM 1.24E-10 ergosterol biosynthesis 3 1.36E-12
8 SGTC_59 0330-0160 6.99 1.3 µM 1.28E-10 22 1.41E-12
9 SGTC_129 0088-0017 6.69 176.6 µM 7.00E-10 25 1.11E-11
10 SGTC_161 4065-0411 5.83 162.2 µM 6.47E-8 28 2.70E-9
11 SGTC_2401 5128683 5.74 200.0 µM 1.01E-7 12 4.61E-9
12 SGTC_87 0100-0052 5.66 35.5 µM 1.52E-7 39 7.59E-9
13 SGTC_8 0354-0001 5.56 70.0 µM 2.49E-7 31 1.38E-8
14 SGTC_2841 9004761 5.37 39.0 µM 5.80E-7 amide catabolism 8 3.85E-8
15 SGTC_17 0960-0276 5.20 49.8 µM 1.25E-6 12 9.74E-8
16 SGTC_2855 9016525 5.16 58.4 µM 1.50E-6 6 1.22E-7
17 SGTC_380 3970-0968 4.86 6.0 µM 5.50E-6 5 5.86E-7
18 SGTC_645 0845-0943 4.39 5.4 µM 3.64E-5 60S ribosome export 3 5.78E-6
19 SGTC_3348 9149464 4.38 3.5 µM 3.70E-5 9 5.88E-6
20 SGTC_1448 4255-0083 4.33 152.0 µM 4.43E-5 ERG2 13 7.32E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.552 1.86E-266 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.483 9.58E-196 YPR153W YPR153W hom Putative protein of unknown function
0.405 2.62E-132 YKL098W MTC2 hom Protein of unknown function; mtc2 is synthetically sick with cdc13-1
0.403 4.88E-131 YPL077C_p YPL077C_p hom Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication
0.275 3.45E-59 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.268 2.25E-56 YHR151C MTC6 hom Protein of unknown function; mtc6 is synthetically sick with cdc13-1
0.259 2.10E-52 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.245 6.17E-47 YML072C TCB3 hom Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.215 1.54E-36 YKL190W CNB1 hom Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress
0.213 1.16E-35 YCL046W_d YCL046W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
0.208 2.94E-34 YBR197C YBR197C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication
0.204 5.18E-33 YPR060C ARO7 hom Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
0.191 6.06E-29 YKL135C APL2 hom Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
0.186 2.00E-27 YLR057W MNL2 hom Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
0.182 2.55E-26 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data