YBR256C / RIB5

Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR256C / RIB5

Click on Significant Values for Screen Details ID:SGTC_25|Compound:0118-0223|FD-Score:3.69|P-value:8.94E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.48|P-value:2.67E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-5.04|P-value:2.46E-7 ID:SGTC_168|Compound:3970-0862|FD-Score:4.03|P-value:2.12E-5 ID:SGTC_323|Compound:4013-0906|FD-Score:4.25|P-value:8.03E-6 ID:SGTC_333|Compound:3486-0131|FD-Score:3.25|P-value:4.67E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:4.29|P-value:6.83E-6 ID:SGTC_778|Compound:3456-2434|FD-Score:-7.36|P-value:9.44E-14 ID:SGTC_816|Compound:0325-0417|FD-Score:-3.18|P-value:8.06E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:3.10|P-value:8.02E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.91|P-value:3.50E-5 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:-4.06|P-value:2.67E-5 ID:SGTC_1573|Compound:oleylamine|FD-Score:-3.60|P-value:1.71E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.99|P-value:2.55E-5 ID:SGTC_1716|Compound:st033144|FD-Score:4.12|P-value:1.41E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-3.72|P-value:1.07E-4 ID:SGTC_1853|Compound:st056710|FD-Score:4.25|P-value:8.14E-6 ID:SGTC_1905|Compound:st060207|FD-Score:3.14|P-value:6.88E-4 ID:SGTC_1990|Compound:st072050|FD-Score:3.21|P-value:5.42E-4 ID:SGTC_1993|Compound:st073995|FD-Score:3.47|P-value:2.05E-4 ID:SGTC_2076|Compound:5212012|FD-Score:3.44|P-value:2.32E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.24|P-value:4.89E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.84|P-value:4.78E-5 ID:SGTC_2352|Compound:9027995|FD-Score:-6.35|P-value:1.14E-10 ID:SGTC_2353|Compound:9059630|FD-Score:-3.72|P-value:1.09E-4 ID:SGTC_2357|Compound:9067248|FD-Score:-7.08|P-value:7.18E-13 ID:SGTC_2428|Compound:5615643|FD-Score:6.30|P-value:9.23E-11 ID:SGTC_2434|Compound:5670830|FD-Score:-3.16|P-value:8.53E-4 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-3.55|P-value:2.10E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.76|P-value:6.71E-5 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.44|P-value:2.35E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-3.42|P-value:3.42E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.40|P-value:2.73E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.52|P-value:2.31E-6 ID:SGTC_2620|Compound:irigenin|FD-Score:3.84|P-value:4.68E-5 ID:SGTC_2723|Compound:salbutamol|FD-Score:3.26|P-value:4.58E-4 ID:SGTC_3036|Compound:9090181|FD-Score:5.02|P-value:1.83E-7 ID:SGTC_3239|Compound:9133757|FD-Score:3.66|P-value:9.74E-5 ID:SGTC_3251|Compound:9135047|FD-Score:-3.33|P-value:4.61E-4 ID:SGTC_3317|Compound:9138495|FD-Score:-4.45|P-value:4.54E-6 ID:SGTC_25|Compound:0118-0223|FD-Score:3.69|P-value:8.94E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.48|P-value:2.67E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-5.04|P-value:2.46E-7 ID:SGTC_168|Compound:3970-0862|FD-Score:4.03|P-value:2.12E-5 ID:SGTC_323|Compound:4013-0906|FD-Score:4.25|P-value:8.03E-6 ID:SGTC_333|Compound:3486-0131|FD-Score:3.25|P-value:4.67E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:4.29|P-value:6.83E-6 ID:SGTC_778|Compound:3456-2434|FD-Score:-7.36|P-value:9.44E-14 ID:SGTC_816|Compound:0325-0417|FD-Score:-3.18|P-value:8.06E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:3.10|P-value:8.02E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.91|P-value:3.50E-5 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:-4.06|P-value:2.67E-5 ID:SGTC_1573|Compound:oleylamine|FD-Score:-3.60|P-value:1.71E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.99|P-value:2.55E-5 ID:SGTC_1716|Compound:st033144|FD-Score:4.12|P-value:1.41E-5 ID:SGTC_1737|Compound:st035557|FD-Score:-3.72|P-value:1.07E-4 ID:SGTC_1853|Compound:st056710|FD-Score:4.25|P-value:8.14E-6 ID:SGTC_1905|Compound:st060207|FD-Score:3.14|P-value:6.88E-4 ID:SGTC_1990|Compound:st072050|FD-Score:3.21|P-value:5.42E-4 ID:SGTC_1993|Compound:st073995|FD-Score:3.47|P-value:2.05E-4 ID:SGTC_2076|Compound:5212012|FD-Score:3.44|P-value:2.32E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.24|P-value:4.89E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.84|P-value:4.78E-5 ID:SGTC_2352|Compound:9027995|FD-Score:-6.35|P-value:1.14E-10 ID:SGTC_2353|Compound:9059630|FD-Score:-3.72|P-value:1.09E-4 ID:SGTC_2357|Compound:9067248|FD-Score:-7.08|P-value:7.18E-13 ID:SGTC_2428|Compound:5615643|FD-Score:6.30|P-value:9.23E-11 ID:SGTC_2434|Compound:5670830|FD-Score:-3.16|P-value:8.53E-4 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-3.55|P-value:2.10E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.76|P-value:6.71E-5 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.44|P-value:2.35E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-3.42|P-value:3.42E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.40|P-value:2.73E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.52|P-value:2.31E-6 ID:SGTC_2620|Compound:irigenin|FD-Score:3.84|P-value:4.68E-5 ID:SGTC_2723|Compound:salbutamol|FD-Score:3.26|P-value:4.58E-4 ID:SGTC_3036|Compound:9090181|FD-Score:5.02|P-value:1.83E-7 ID:SGTC_3239|Compound:9133757|FD-Score:3.66|P-value:9.74E-5 ID:SGTC_3251|Compound:9135047|FD-Score:-3.33|P-value:4.61E-4 ID:SGTC_3317|Compound:9138495|FD-Score:-4.45|P-value:4.54E-6

Top fitness defect scores for YBR256C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2428 5615643 6.30 7.8 µM 9.23E-11 2 1.51E-10 0.23
2 SGTC_3036 9090181 5.02 49.5 µM 1.83E-7 2 2.61E-7 0.83
3 SGTC_2599 2',3-dihydroxy-4,4',6'-trimethoxychalcone 4.52 100.0 µM 2.31E-6 4 3.16E-6 0.01
4 SGTC_426 0988-0037 4.29 14.6 µM 6.83E-6 ERAD & cell cycle 10 9.13E-6 0.17
5 SGTC_323 4013-0906 4.25 34.3 µM 8.03E-6 ERG2 2 1.07E-5 0.60
6 SGTC_1853 st056710 4.25 91.2 µM 8.14E-6 amide catabolism 5 1.09E-5 0.32
7 SGTC_1716 st033144 4.12 30.6 µM 1.41E-5 6 1.86E-5 0.31
8 SGTC_168 3970-0862 4.03 530.5 µM 2.12E-5 43 2.78E-5 0.03
9 SGTC_1715 st032160 3.99 10.2 µM 2.55E-5 5 3.32E-5 0.17
10 SGTC_1464 k208-0027 3.91 110.0 µM 3.50E-5 6 4.54E-5 0.47
11 SGTC_2620 irigenin 3.84 100.0 µM 4.68E-5 16 6.03E-5 0.15
12 SGTC_2350 9023895 3.84 38.9 µM 4.78E-5 ergosterol biosynthesis 9 6.16E-5 0.11
13 SGTC_2568 diosmetin 3.76 100.0 µM 6.71E-5 13 8.59E-5 0.04
14 SGTC_25 0118-0223 3.69 35.7 µM 8.94E-5 3 1.14E-4 0.39
15 SGTC_3239 9133757 3.66 49.5 µM 9.74E-5 1 1.24E-4 0.52
16 SGTC_1993 st073995 3.47 70.8 µM 2.05E-4 9 2.56E-4 0.04
17 SGTC_2076 5212012 3.44 200.0 µM 2.32E-4 3 2.89E-4 0.37
18 SGTC_2569 tetrandrine 3.44 100.0 µM 2.35E-4 8 2.93E-4 0.02
19 SGTC_2596 sinensetin 3.40 100.0 µM 2.73E-4 12 3.40E-4 0.09
20 SGTC_2723 salbutamol 3.26 83.6 µM 4.58E-4 2 5.64E-4 0.37

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.182 1.84E-26 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.127 1.69E-13 YER136W GDI1 het GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
0.123 8.18E-13 YCR048W ARE1 hom Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication
0.123 8.76E-13 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.119 4.30E-12 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.115 2.16E-11 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
0.113 5.55E-11 YGR086C PIL1 hom Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress
0.110 1.50E-10 YKL164C PIR1 hom O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
0.110 1.99E-10 YGR207C CIR1 hom Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
0.106 7.22E-10 YLR127C APC2 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
0.103 1.87E-9 YDR044W HEM13 het Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)
0.103 2.15E-9 YDL209C CWC2 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
0.103 2.56E-9 YNL132W KRE33 het Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
0.101 4.29E-9 YGL208W SIP2 hom One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane
0.100 6.68E-9 YDR277C MTH1 hom Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation