YBR263W / SHM1

Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR263W / SHM1

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:2.80|P-value:7.66E-4 ID:SGTC_84|Compound:0443-0269|FD-Score:2.93|P-value:4.71E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:2.89|P-value:5.54E-4 ID:SGTC_270|Compound:tamoxifen|FD-Score:2.84|P-value:6.60E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:4.05|P-value:2.77E-6 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:3.22|P-value:1.45E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.57|P-value:5.38E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:3.20|P-value:1.55E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:2.97|P-value:4.03E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:3.99|P-value:3.67E-6 ID:SGTC_828|Compound:2074-0507|FD-Score:3.29|P-value:1.07E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:-3.07|P-value:4.42E-4 ID:SGTC_993|Compound:3909-8757|FD-Score:-3.21|P-value:2.53E-4 ID:SGTC_1101|Compound:ebselen|FD-Score:3.04|P-value:3.07E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.24|P-value:1.92E-6 ID:SGTC_1191|Compound:1487-1166|FD-Score:4.18|P-value:1.36E-6 ID:SGTC_1197|Compound:1601-0112|FD-Score:-3.91|P-value:1.05E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:5.66|P-value:1.24E-10 ID:SGTC_1201|Compound:1889-3372|FD-Score:4.93|P-value:1.70E-8 ID:SGTC_1216|Compound:1165-0504|FD-Score:2.95|P-value:4.28E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:2.91|P-value:4.99E-4 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.52|P-value:3.82E-5 ID:SGTC_1456|Compound:k015-0012|FD-Score:2.85|P-value:6.35E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:-2.86|P-value:9.88E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.08|P-value:2.61E-4 ID:SGTC_1620|Compound:st003704|FD-Score:3.64|P-value:2.13E-5 ID:SGTC_1675|Compound:st018485|FD-Score:2.76|P-value:9.17E-4 ID:SGTC_1925|Compound:st057770|FD-Score:-3.17|P-value:3.02E-4 ID:SGTC_2166|Compound:5933061|FD-Score:2.99|P-value:3.65E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:-2.89|P-value:9.00E-4 ID:SGTC_2331|Compound:7980721|FD-Score:2.74|P-value:9.73E-4 ID:SGTC_2362|Compound:9071156|FD-Score:5.69|P-value:1.05E-10 ID:SGTC_2414|Compound:st077232|FD-Score:-3.07|P-value:4.40E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.40|P-value:1.12E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:-2.94|P-value:7.42E-4 ID:SGTC_2439|Compound:5492030|FD-Score:3.01|P-value:3.39E-4 ID:SGTC_2706|Compound:st078239|FD-Score:-2.87|P-value:9.74E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.79|P-value:1.91E-5 ID:SGTC_3004|Compound:9076122|FD-Score:3.19|P-value:1.65E-4 ID:SGTC_3315|Compound:9136395|FD-Score:2.89|P-value:5.41E-4 ID:SGTC_3334|Compound:9142812|FD-Score:2.82|P-value:7.13E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:2.80|P-value:7.66E-4 ID:SGTC_84|Compound:0443-0269|FD-Score:2.93|P-value:4.71E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:2.89|P-value:5.54E-4 ID:SGTC_270|Compound:tamoxifen|FD-Score:2.84|P-value:6.60E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:4.05|P-value:2.77E-6 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:3.22|P-value:1.45E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.57|P-value:5.38E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:3.20|P-value:1.55E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:2.97|P-value:4.03E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:3.99|P-value:3.67E-6 ID:SGTC_828|Compound:2074-0507|FD-Score:3.29|P-value:1.07E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:-3.07|P-value:4.42E-4 ID:SGTC_993|Compound:3909-8757|FD-Score:-3.21|P-value:2.53E-4 ID:SGTC_1101|Compound:ebselen|FD-Score:3.04|P-value:3.07E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.24|P-value:1.92E-6 ID:SGTC_1191|Compound:1487-1166|FD-Score:4.18|P-value:1.36E-6 ID:SGTC_1197|Compound:1601-0112|FD-Score:-3.91|P-value:1.05E-5 ID:SGTC_1200|Compound:1643-0193|FD-Score:5.66|P-value:1.24E-10 ID:SGTC_1201|Compound:1889-3372|FD-Score:4.93|P-value:1.70E-8 ID:SGTC_1216|Compound:1165-0504|FD-Score:2.95|P-value:4.28E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:2.91|P-value:4.99E-4 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.52|P-value:3.82E-5 ID:SGTC_1456|Compound:k015-0012|FD-Score:2.85|P-value:6.35E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:-2.86|P-value:9.88E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.08|P-value:2.61E-4 ID:SGTC_1620|Compound:st003704|FD-Score:3.64|P-value:2.13E-5 ID:SGTC_1675|Compound:st018485|FD-Score:2.76|P-value:9.17E-4 ID:SGTC_1925|Compound:st057770|FD-Score:-3.17|P-value:3.02E-4 ID:SGTC_2166|Compound:5933061|FD-Score:2.99|P-value:3.65E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:-2.89|P-value:9.00E-4 ID:SGTC_2331|Compound:7980721|FD-Score:2.74|P-value:9.73E-4 ID:SGTC_2362|Compound:9071156|FD-Score:5.69|P-value:1.05E-10 ID:SGTC_2414|Compound:st077232|FD-Score:-3.07|P-value:4.40E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.40|P-value:1.12E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:-2.94|P-value:7.42E-4 ID:SGTC_2439|Compound:5492030|FD-Score:3.01|P-value:3.39E-4 ID:SGTC_2706|Compound:st078239|FD-Score:-2.87|P-value:9.74E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-3.79|P-value:1.91E-5 ID:SGTC_3004|Compound:9076122|FD-Score:3.19|P-value:1.65E-4 ID:SGTC_3315|Compound:9136395|FD-Score:2.89|P-value:5.41E-4 ID:SGTC_3334|Compound:9142812|FD-Score:2.82|P-value:7.13E-4

Top fitness defect scores for YBR263W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2362 9071156 5.69 126.0 µM 1.05E-10 4 6.52E-9
2 SGTC_1200 1643-0193 5.66 18.4 µM 1.24E-10 3 7.48E-9
3 SGTC_1201 1889-3372 4.93 45.9 µM 1.70E-8 Golgi 3 4.09E-7
4 SGTC_1191 1487-1166 4.18 10.6 µM 1.36E-6 azole & statin 27 1.45E-5
5 SGTC_356 1634-0012 4.05 32.5 µM 2.77E-6 22 2.59E-5
6 SGTC_807 1315-0151 3.99 48.0 µM 3.67E-6 26 3.26E-5
7 SGTC_1620 st003704 3.64 16.5 µM 2.13E-5 26 1.36E-4
8 SGTC_1382 3002-2117 3.52 298.0 µM 3.82E-5 20 2.19E-4
9 SGTC_828 2074-0507 3.29 139.0 µM 1.07E-4 RPP1 & pyrimidine depletion 47 5.08E-4
10 SGTC_388 3',4'-dichloroisocoumarin 3.22 16.7 µM 1.45E-4 33 6.50E-4
11 SGTC_530 4358-1714 3.20 28.0 µM 1.55E-4 77 6.87E-4
12 SGTC_3004 9076122 3.19 71.4 µM 1.65E-4 41 7.21E-4
13 SGTC_1562 morin hydrate 3.08 59.1 µM 2.61E-4 55 0.00105
14 SGTC_1101 ebselen 3.04 5.3 µM 3.07E-4 57 0.00120
15 SGTC_2439 5492030 3.01 7.2 µM 3.39E-4 42 0.00130
16 SGTC_2166 5933061 2.99 31.5 µM 3.65E-4 tubulin folding & SWR complex 90 0.00138
17 SGTC_620 0109-0052 2.97 432.0 µM 4.03E-4 58 0.00150
18 SGTC_1216 1165-0504 2.95 9.7 µM 4.28E-4 54 0.00157
19 SGTC_84 0443-0269 2.93 128.8 µM 4.71E-4 62 0.00170
20 SGTC_1217 1183-1422 2.91 255.0 µM 4.99E-4 56 0.00178

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.147 1.29E-17 YEL066W HPA3 hom D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro
0.142 1.49E-16 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.138 1.05E-15 YML059C NTE1 hom Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes
0.127 1.30E-13 YDL182W LYS20 hom Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication
0.126 2.28E-13 YFR052W RPN12 het Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
0.125 3.37E-13 YNL078W NIS1 hom Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network
0.125 4.35E-13 YDR242W AMD2 hom Putative amidase
0.122 1.50E-12 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.121 1.63E-12 YLL012W YEH1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.120 2.73E-12 YOR081C TGL5 hom Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate
0.118 5.67E-12 YKL059C MPE1 het Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
0.114 3.00E-11 YNL312W RFA2 het Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
0.114 3.09E-11 YKL189W HYM1 het Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response
0.112 6.79E-11 YDR089W YDR089W hom Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.112 8.05E-11 YLR272C YCS4 het Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation