YBR265W / TSC10

3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBR265W / TSC10

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.61|P-value:3.65E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:4.24|P-value:3.52E-5 ID:SGTC_188|Compound:3229-0966|FD-Score:3.75|P-value:2.22E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:4.31|P-value:2.73E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:4.28|P-value:3.06E-5 ID:SGTC_814|Compound:3342-0569|FD-Score:3.61|P-value:3.60E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:5.01|P-value:1.31E-6 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:-3.87|P-value:1.40E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.99|P-value:9.34E-5 ID:SGTC_1864|Compound:st057645|FD-Score:3.42|P-value:6.76E-4 ID:SGTC_1936|Compound:st074407|FD-Score:4.16|P-value:4.92E-5 ID:SGTC_2067|Compound:5224629|FD-Score:3.40|P-value:7.14E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:3.77|P-value:2.05E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.40|P-value:7.13E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:-4.40|P-value:1.82E-5 ID:SGTC_2601|Compound:securinine|FD-Score:-6.74|P-value:1.23E-10 ID:SGTC_2602|Compound:kawain|FD-Score:-8.25|P-value:4.94E-15 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-5.67|P-value:5.08E-8 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.97|P-value:9.91E-5 ID:SGTC_2942|Compound:9052777|FD-Score:4.84|P-value:2.90E-6 ID:SGTC_2952|Compound:9073731|FD-Score:4.02|P-value:8.29E-5 ID:SGTC_2956|Compound:9076196|FD-Score:3.50|P-value:5.17E-4 ID:SGTC_3019|Compound:9081971|FD-Score:3.39|P-value:7.41E-4 ID:SGTC_3069|Compound:9117009|FD-Score:-5.08|P-value:9.36E-7 ID:SGTC_3071|Compound:9117417|FD-Score:-5.56|P-value:9.02E-8 ID:SGTC_3073|Compound:9114972|FD-Score:-3.71|P-value:2.46E-4 ID:SGTC_3077|Compound:9117319|FD-Score:5.37|P-value:2.46E-7 ID:SGTC_3211|Compound:9128781|FD-Score:7.71|P-value:2.37E-13 ID:SGTC_3269|Compound:9137823|FD-Score:-3.75|P-value:2.17E-4 ID:SGTC_3270|Compound:9137563|FD-Score:-3.35|P-value:8.13E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.61|P-value:3.65E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:4.24|P-value:3.52E-5 ID:SGTC_188|Compound:3229-0966|FD-Score:3.75|P-value:2.22E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:4.31|P-value:2.73E-5 ID:SGTC_574|Compound:1315-0397|FD-Score:4.28|P-value:3.06E-5 ID:SGTC_814|Compound:3342-0569|FD-Score:3.61|P-value:3.60E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:5.01|P-value:1.31E-6 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:-3.87|P-value:1.40E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.99|P-value:9.34E-5 ID:SGTC_1864|Compound:st057645|FD-Score:3.42|P-value:6.76E-4 ID:SGTC_1936|Compound:st074407|FD-Score:4.16|P-value:4.92E-5 ID:SGTC_2067|Compound:5224629|FD-Score:3.40|P-value:7.14E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:3.77|P-value:2.05E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.40|P-value:7.13E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:-4.40|P-value:1.82E-5 ID:SGTC_2601|Compound:securinine|FD-Score:-6.74|P-value:1.23E-10 ID:SGTC_2602|Compound:kawain|FD-Score:-8.25|P-value:4.94E-15 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-5.67|P-value:5.08E-8 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.97|P-value:9.91E-5 ID:SGTC_2942|Compound:9052777|FD-Score:4.84|P-value:2.90E-6 ID:SGTC_2952|Compound:9073731|FD-Score:4.02|P-value:8.29E-5 ID:SGTC_2956|Compound:9076196|FD-Score:3.50|P-value:5.17E-4 ID:SGTC_3019|Compound:9081971|FD-Score:3.39|P-value:7.41E-4 ID:SGTC_3069|Compound:9117009|FD-Score:-5.08|P-value:9.36E-7 ID:SGTC_3071|Compound:9117417|FD-Score:-5.56|P-value:9.02E-8 ID:SGTC_3073|Compound:9114972|FD-Score:-3.71|P-value:2.46E-4 ID:SGTC_3077|Compound:9117319|FD-Score:5.37|P-value:2.46E-7 ID:SGTC_3211|Compound:9128781|FD-Score:7.71|P-value:2.37E-13 ID:SGTC_3269|Compound:9137823|FD-Score:-3.75|P-value:2.17E-4 ID:SGTC_3270|Compound:9137563|FD-Score:-3.35|P-value:8.13E-4

Top fitness defect scores for YBR265W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3211 9128781 7.71 49.5 µM 2.37E-13 1 6.09E-15 1.05
2 SGTC_3077 9117319 5.37 49.5 µM 2.46E-7 2 4.03E-8 0.56
3 SGTC_1159 4092-0538 5.01 61.2 µM 1.31E-6 1 2.67E-7 0.81
4 SGTC_2942 9052777 4.84 62.2 µM 2.90E-6 4 6.58E-7 0.28
5 SGTC_543 3844-1095 4.31 191.0 µM 2.73E-5 ERG2 4 8.32E-6 0.16
6 SGTC_574 1315-0397 4.28 40.0 µM 3.06E-5 8 9.46E-6 0.27
7 SGTC_177 1327-0068 4.24 8.4 µM 3.52E-5 20 1.11E-5 0.17
8 SGTC_1936 st074407 4.16 74.3 µM 4.92E-5 DNA damage response 10 1.62E-5 0.34
9 SGTC_2952 9073731 4.02 13.8 µM 8.29E-5 tubulin folding & SWR complex 14 2.92E-5 0.65
10 SGTC_1680 diphenhydramine 3.99 100.0 µM 9.34E-5 NEO1 15 3.34E-5 0.05
11 SGTC_2758 dehydroepiandrosterone 3.97 69.3 µM 9.91E-5 7 3.57E-5 0.09
12 SGTC_2571 chrysanthemic acid 3.77 100.0 µM 2.05E-4 7 8.10E-5 0.44
13 SGTC_188 3229-0966 3.75 278.5 µM 2.22E-4 21 8.89E-5 0.11
14 SGTC_814 3342-0569 3.61 96.0 µM 3.60E-4 3 1.53E-4 0.13
15 SGTC_6 0844-0013 3.61 19.8 µM 3.65E-4 13 1.56E-4 0.10
16 SGTC_2956 9076196 3.50 2.5 µM 5.17E-4 5 2.31E-4 0.14
17 SGTC_1864 st057645 3.42 30.4 µM 6.76E-4 amide catabolism 5 3.12E-4 0.10
18 SGTC_2592 piperlongumine 3.40 83.3 µM 7.13E-4 TSC3-RPN4 13 3.31E-4 0.06
19 SGTC_2067 5224629 3.40 34.1 µM 7.14E-4 18 3.32E-4 0.18
20 SGTC_3019 9081971 3.39 49.5 µM 7.41E-4 9 3.46E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.216 7.61E-37 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.172 8.55E-24 YCR006C_d YCR006C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.166 4.16E-22 YJR148W BAT2 hom Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase
0.133 8.52E-15 YMR191W SPG5 hom Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.125 3.68E-13 YDL028C MPS1 het Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p
0.121 1.61E-12 YGR122C-A_d YGR122C-A_d hom Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W
0.120 3.88E-12 YOL140W ARG8 hom Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
0.115 2.05E-11 YHR193C EGD2 hom Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes
0.108 3.13E-10 YDR197W CBS2 hom Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
0.101 5.14E-9 YLR454W_p FMP27_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.098 1.23E-8 YLR230W_d YLR230W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
0.096 2.70E-8 YDR179C CSN9 hom Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling
0.096 2.76E-8 YOR353C SOG2 het Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
0.094 5.37E-8 YJL106W IME2 hom Serine/threonine protein kinase involved in activation of meiosis, associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p
0.092 9.40E-8 YPL265W DIP5 hom Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress