HIPHOP chemogenomics database

YBR272C / HSM3

Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation

Zygosity: Homozygous strain

fixedexpanded
Profile for YBR272C / HSM3

Click on Significant Values for Screen Details

Top fitness defect scores for YBR272C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2953	9074162	6.06	66.5 µM	6.91E-11		4	6.85E-10
2	SGTC_1756	st041558	5.94	35.5 µM	1.58E-10		1	1.44E-9
3	SGTC_2878	9043973	5.71	26.0 µM	7.46E-10		1	5.75E-9
4	SGTC_2666	tannic acid	5.44	100.0 µM	4.16E-9	iron homeostasis	13	2.67E-8
5	SGTC_921	1561-0023	5.24	372.0 µM	1.43E-8		4	8.05E-8
6	SGTC_2874	9043904	5.14	71.4 µM	2.64E-8		1	1.39E-7
7	SGTC_2917	7979103	5.07	53.8 µM	3.95E-8		9	1.99E-7
8	SGTC_2971	9092186	4.21	9.9 µM	4.06E-6	ergosterol biosynthesis	23	1.26E-5
9	SGTC_1107	amlodipine	4.20	47.0 µM	4.28E-6		25	1.32E-5
10	SGTC_310	1273-0058	4.06	1.1 µM	8.39E-6	mitochondrial stress	238	2.41E-5
11	SGTC_1758	st042942	4.04	75.2 µM	9.31E-6		18	2.65E-5
12	SGTC_2919	7978188	3.73	46.4 µM	3.89E-5		28	9.54E-5
13	SGTC_2247	cerulenin	3.65	830.0 nM	5.48E-5		25	1.30E-4
14	SGTC_3066	9117245	3.57	49.5 µM	7.88E-5		19	1.80E-4
15	SGTC_924	2046-0044	3.51	21.3 µM	1.00E-4		22	2.23E-4
16	SGTC_3254	9136651	3.49	49.5 µM	1.10E-4	DNA damage response	38	2.43E-4
17	SGTC_2516	gossypol	3.47	71.5 µM	1.21E-4		27	2.64E-4
18	SGTC_3060	1327-0027	3.38	12.5 µM	1.74E-4		50	3.67E-4
19	SGTC_762	1165-0429	3.33	115.0 µM	2.14E-4	TSC3-RPN4	41	4.41E-4
20	SGTC_2344	7990960	3.29	77.0 µM	2.49E-4		47	5.05E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.160	1.70E-20	YGR077C	PEX8	hom	Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.134	6.10E-15	YLR390W-A	CCW14	hom	Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall
0.106	8.55E-10	YBR274W	CHK1	hom	Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
0.100	6.22E-9	YDR497C	ITR1	hom	Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress
0.097	2.21E-8	YIL010W	DOT5	hom	Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth
0.094	5.81E-8	YDR517W	GRH1	hom	Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress
0.093	6.76E-8	YOR384W	FRE5	hom	Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.088	3.20E-7	YOR382W	FIT2	hom	Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.086	6.55E-7	YPL116W	HOS3	hom	Trichostatin A-insensitive homodimeric histone deacetylase (HDAC) with specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats
0.083	1.51E-6	YDR193W_d	YDR193W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.082	1.84E-6	YNR037C	RSM19	hom	Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein
0.082	2.07E-6	YBL018C	POP8	het	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.082	2.23E-6	YDR034C	LYS14	hom	Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer
0.080	3.85E-6	YGR022C_d	YGR022C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.078	6.48E-6	YPL177C	CUP9	hom	Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress