YBR274W / CHK1

Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR274W / CHK1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:4.64|P-value:3.35E-7 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.10|P-value:4.52E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:3.46|P-value:1.06E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:3.44|P-value:1.16E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:3.14|P-value:3.82E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:-3.14|P-value:3.69E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.49|P-value:9.24E-5 ID:SGTC_1271|Compound:0832-2808|FD-Score:-3.12|P-value:3.96E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.30|P-value:2.03E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:-3.23|P-value:2.57E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:-3.03|P-value:5.57E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-6.33|P-value:5.17E-12 ID:SGTC_1463|Compound:k081-0032|FD-Score:-6.27|P-value:8.45E-12 ID:SGTC_1725|Compound:st036284|FD-Score:3.20|P-value:3.06E-4 ID:SGTC_2056|Compound:5235370|FD-Score:-3.25|P-value:2.42E-4 ID:SGTC_2076|Compound:5212012|FD-Score:3.31|P-value:2.02E-4 ID:SGTC_2080|Compound:5212524|FD-Score:3.10|P-value:4.49E-4 ID:SGTC_2085|Compound:5307070|FD-Score:3.23|P-value:2.73E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-3.04|P-value:5.44E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.58|P-value:6.48E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-3.30|P-value:1.93E-4 ID:SGTC_2691|Compound:st077356|FD-Score:-3.02|P-value:5.87E-4 ID:SGTC_2884|Compound:9058502|FD-Score:-3.22|P-value:2.63E-4 ID:SGTC_2888|Compound:9058547|FD-Score:-3.26|P-value:2.29E-4 ID:SGTC_2949|Compound:9072542|FD-Score:-3.52|P-value:7.81E-5 ID:SGTC_2951|Compound:9072950|FD-Score:-6.36|P-value:4.38E-12 ID:SGTC_3143|Compound:9086658|FD-Score:2.94|P-value:8.35E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:4.64|P-value:3.35E-7 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.10|P-value:4.52E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:3.46|P-value:1.06E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:3.44|P-value:1.16E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:3.14|P-value:3.82E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:-3.14|P-value:3.69E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.49|P-value:9.24E-5 ID:SGTC_1271|Compound:0832-2808|FD-Score:-3.12|P-value:3.96E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.30|P-value:2.03E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:-3.23|P-value:2.57E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:-3.03|P-value:5.57E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-6.33|P-value:5.17E-12 ID:SGTC_1463|Compound:k081-0032|FD-Score:-6.27|P-value:8.45E-12 ID:SGTC_1725|Compound:st036284|FD-Score:3.20|P-value:3.06E-4 ID:SGTC_2056|Compound:5235370|FD-Score:-3.25|P-value:2.42E-4 ID:SGTC_2076|Compound:5212012|FD-Score:3.31|P-value:2.02E-4 ID:SGTC_2080|Compound:5212524|FD-Score:3.10|P-value:4.49E-4 ID:SGTC_2085|Compound:5307070|FD-Score:3.23|P-value:2.73E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-3.04|P-value:5.44E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.58|P-value:6.48E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-3.30|P-value:1.93E-4 ID:SGTC_2691|Compound:st077356|FD-Score:-3.02|P-value:5.87E-4 ID:SGTC_2884|Compound:9058502|FD-Score:-3.22|P-value:2.63E-4 ID:SGTC_2888|Compound:9058547|FD-Score:-3.26|P-value:2.29E-4 ID:SGTC_2949|Compound:9072542|FD-Score:-3.52|P-value:7.81E-5 ID:SGTC_2951|Compound:9072950|FD-Score:-6.36|P-value:4.38E-12 ID:SGTC_3143|Compound:9086658|FD-Score:2.94|P-value:8.35E-4

Top fitness defect scores for YBR274W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_6 0844-0013 4.64 19.8 µM 3.35E-7 51 1.74E-6
2 SGTC_2419 paclitaxel 3.58 166.5 µM 6.48E-5 59 1.75E-4
3 SGTC_1262 0737-0114 3.49 49.6 µM 9.24E-5 37 2.39E-4
4 SGTC_626 0214-0009 3.46 33.0 µM 1.06E-4 copper-dependent oxidative stress 34 2.69E-4
5 SGTC_669 0108-0021 3.44 26.2 µM 1.16E-4 fatty acid desaturase (OLE1) 116 2.91E-4
6 SGTC_2076 5212012 3.31 200.0 µM 2.02E-4 16 4.74E-4
7 SGTC_1348 1493-0373 3.30 93.8 µM 2.03E-4 31 4.76E-4
8 SGTC_2085 5307070 3.23 144.1 µM 2.73E-4 superoxide 53 6.19E-4
9 SGTC_1725 st036284 3.20 20.6 µM 3.06E-4 62 6.83E-4
10 SGTC_749 1000-0129 3.14 128.0 µM 3.82E-4 amide catabolism 53 8.31E-4
11 SGTC_2080 5212524 3.10 12.4 µM 4.49E-4 copper-dependent oxidative stress 43 9.57E-4
12 SGTC_261 5-fluorouridine 3.10 15.4 µM 4.52E-4 exosome 48 9.63E-4
13 SGTC_3143 9086658 2.94 49.5 µM 8.35E-4 109 0.00165
14 SGTC_2599 2',3-dihydroxy-4,4',6'-trimethoxychalcone 2.82 100.0 µM 0.00130 93 0.00243
15 SGTC_510 ag-490 2.79 170.0 µM 0.00140 30 0.00261
16 SGTC_476 mead acid 2.79 10.0 µM 0.00144 29 0.00266
17 SGTC_1289 0976-0073 2.79 69.1 µM 0.00144 66 0.00267
18 SGTC_2534 helenine 2.76 20.1 µM 0.00157 RSC complex & mRNA processing 51 0.00289
19 SGTC_2537 larixol 2.75 68.9 µM 0.00163 100 0.00299
20 SGTC_2074 5212007 2.75 75.4 µM 0.00166 56 0.00302

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.378 3.09E-114 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.328 4.88E-85 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.323 3.77E-82 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.311 8.98E-76 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.302 1.65E-71 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.296 5.04E-69 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.278 1.59E-60 YNL218W MGS1 hom Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.277 2.70E-60 YMR107W SPG4 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.275 3.36E-59 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.273 1.67E-58 YDR530C APA2 hom Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
0.264 1.14E-54 YLR179C YLR179C hom Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
0.263 4.73E-54 YJR146W_p YJR146W_p hom Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
0.257 9.61E-52 YLL054C_p YLL054C_p hom Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
0.256 3.18E-51 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.252 8.07E-50 YJL132W_p YJL132W_p hom Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene