HIPHOP chemogenomics database

YBR277C_d

Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W

Zygosity: Homozygous strain

fixedexpanded
Profile for YBR277C / YBR277C

Click on Significant Values for Screen Details

Top fitness defect scores for YBR277C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2009	4012297	8.41	12.1 µM	1.61E-17	cell wall signaling	12	2.08E-17
2	SGTC_921	1561-0023	6.73	372.0 µM	7.30E-12		1	8.33E-12
3	SGTC_58	0957-0393	6.23	9.0 µM	2.10E-10	cell wall	203	2.32E-10
4	SGTC_2026	5148700	6.20	152.0 µM	2.55E-10		5	2.81E-10
5	SGTC_3232	9132874	5.21	49.5 µM	9.06E-8	RPP1 & pyrimidine depletion	4	9.52E-8
6	SGTC_450	aminopyrene	5.20	156.0 nM	9.23E-8		12	9.70E-8
7	SGTC_2005	4024620	5.01	188.0 µM	2.64E-7		6	2.76E-7
8	SGTC_1387	3342-1166	4.92	1.8 µM	4.10E-7	mitochondrial processes	5	4.25E-7
9	SGTC_1031	k035-0009	4.88	16.0 µM	5.21E-7		11	5.40E-7
10	SGTC_454	lyso-paf C-16	4.84	6.7 µM	6.20E-7		5	6.41E-7
11	SGTC_2165	5727197	4.82	32.1 µM	7.11E-7		21	7.35E-7
12	SGTC_3248	9135562	4.77	49.5 µM	8.91E-7		2	9.20E-7
13	SGTC_2002	4023503	4.75	131.0 µM	9.97E-7	mitochondrial processes	15	1.03E-6
14	SGTC_2011	4012611	4.74	177.0 µM	1.01E-6	mitochondrial processes	15	1.05E-6
15	SGTC_1377	2944-0135	4.63	198.0 µM	1.79E-6	calcium & mitochondrial duress	20	1.84E-6
16	SGTC_3244	9134489	4.46	49.5 µM	4.10E-6		10	4.18E-6
17	SGTC_1537	2',6'-dihydroxyflavanone	4.45	78.0 µM	4.24E-6		10	4.32E-6
18	SGTC_1721	st082152	4.37	9.3 µM	6.20E-6	60S ribosome export	13	6.30E-6
19	SGTC_3038	9091290	4.33	49.5 µM	7.38E-6		10	7.49E-6
20	SGTC_2584	androsterone	4.32	48.7 µM	7.75E-6	cell wall	105	7.87E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.143	7.25E-17	YOR231W	MKK1	hom	MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.143	9.29E-17	YGR196C	FYV8	hom	Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.141	1.82E-16	YBR260C	RGD1	hom	GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.136	2.86E-15	YDL203C	ACK1	hom	Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.136	2.91E-15	YLR331C_d	JIP3_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.126	2.38E-13	YIL160C	POT1	hom	3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.125	3.33E-13	YIL148W	RPL40A	hom	Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.122	1.21E-12	YJR054W	KCH1	hom	Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.119	4.19E-12	YLR332W	MID2	hom	O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.115	2.33E-11	YDR205W	MSC2	hom	Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.113	4.84E-11	YDL025C	RTK1	hom	Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
0.112	7.20E-11	YNL314W	DAL82	hom	Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.112	8.61E-11	YJL125C	GCD14	het	Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
0.107	4.76E-10	YJL020C	BBC1	hom	Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches
0.107	4.80E-10	YCR079W	PTC6	hom	Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase