YBR288C / APM3

Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR288C / APM3

Click on Significant Values for Screen Details ID:SGTC_89|Compound:0302-0250|FD-Score:3.52|P-value:7.08E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:4.36|P-value:3.85E-5 ID:SGTC_123|Compound:0083-0120|FD-Score:4.40|P-value:3.28E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:3.93|P-value:1.79E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:-4.18|P-value:7.16E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:11.00|P-value:1.21E-23 ID:SGTC_300|Compound:r073-0011|FD-Score:4.21|P-value:6.78E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:5.63|P-value:1.62E-7 ID:SGTC_351|Compound:caspofungin|FD-Score:3.61|P-value:5.23E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:9.27|P-value:1.93E-17 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.68|P-value:4.14E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:7.27|P-value:2.01E-11 ID:SGTC_445|Compound:e6 berbamine|FD-Score:10.10|P-value:3.56E-20 ID:SGTC_488|Compound:nitrendipine|FD-Score:4.19|P-value:7.27E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:8.69|P-value:1.47E-15 ID:SGTC_528|Compound:3492-0019|FD-Score:16.40|P-value:1.27E-50 ID:SGTC_537|Compound:1334-0398|FD-Score:-3.82|P-value:2.57E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:4.73|P-value:8.69E-6 ID:SGTC_841|Compound:0251-0215|FD-Score:10.40|P-value:1.28E-21 ID:SGTC_892|Compound:0929-0076|FD-Score:3.57|P-value:6.05E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:-3.77|P-value:3.02E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.43|P-value:9.37E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:-3.72|P-value:3.53E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:-3.41|P-value:9.60E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:3.57|P-value:6.07E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:4.04|P-value:1.23E-4 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:4.12|P-value:9.21E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.93|P-value:1.81E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:-4.01|P-value:1.33E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:4.37|P-value:3.69E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:4.38|P-value:3.51E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:5.47|P-value:3.46E-7 ID:SGTC_1251|Compound:0490-5699|FD-Score:-3.58|P-value:5.63E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:4.42|P-value:3.01E-5 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.63|P-value:4.98E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:4.39|P-value:3.38E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.72|P-value:3.70E-4 ID:SGTC_1383|Compound:3049-0032|FD-Score:-5.95|P-value:3.18E-8 ID:SGTC_1388|Compound:3346-4487|FD-Score:3.61|P-value:5.25E-4 ID:SGTC_1403|Compound:3909-7815|FD-Score:10.40|P-value:2.62E-21 ID:SGTC_1448|Compound:4255-0083|FD-Score:4.10|P-value:1.00E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:4.69|P-value:1.06E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.51|P-value:2.15E-5 ID:SGTC_1581|Compound:himbacine|FD-Score:4.79|P-value:6.79E-6 ID:SGTC_1658|Compound:st012944|FD-Score:4.13|P-value:8.85E-5 ID:SGTC_1687|Compound:st020778|FD-Score:8.31|P-value:2.22E-14 ID:SGTC_1730|Compound:st033808|FD-Score:-3.50|P-value:7.25E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-4.20|P-value:6.67E-5 ID:SGTC_1744|Compound:st037937|FD-Score:-3.50|P-value:7.37E-4 ID:SGTC_1769|Compound:st044406|FD-Score:3.69|P-value:4.05E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.57|P-value:5.96E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:7.45|P-value:6.86E-12 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.48|P-value:2.41E-5 ID:SGTC_1962|Compound:st076595|FD-Score:3.55|P-value:6.50E-4 ID:SGTC_2030|Compound:5175110|FD-Score:5.69|P-value:1.19E-7 ID:SGTC_2046|Compound:4110841|FD-Score:6.08|P-value:1.75E-8 ID:SGTC_2105|Compound:5350118|FD-Score:-3.78|P-value:2.96E-4 ID:SGTC_2291|Compound:7917025|FD-Score:6.19|P-value:9.57E-9 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:4.36|P-value:3.78E-5 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.99|P-value:1.42E-4 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.97|P-value:1.57E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.28|P-value:8.36E-7 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:3.51|P-value:7.35E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.39|P-value:3.36E-5 ID:SGTC_2779|Compound:methotrexate|FD-Score:12.20|P-value:7.94E-29 ID:SGTC_2810|Compound:7959871|FD-Score:3.79|P-value:2.97E-4 ID:SGTC_2923|Compound:7992654|FD-Score:3.84|P-value:2.45E-4 ID:SGTC_2930|Compound:9013230|FD-Score:4.76|P-value:7.96E-6 ID:SGTC_3029|Compound:9088683|FD-Score:3.65|P-value:4.59E-4 ID:SGTC_3141|Compound:9094609|FD-Score:3.48|P-value:7.86E-4 ID:SGTC_3227|Compound:9130768|FD-Score:4.61|P-value:1.45E-5 ID:SGTC_3305|Compound:9127027|FD-Score:-3.60|P-value:5.33E-4 ID:SGTC_89|Compound:0302-0250|FD-Score:3.52|P-value:7.08E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:4.36|P-value:3.85E-5 ID:SGTC_123|Compound:0083-0120|FD-Score:4.40|P-value:3.28E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:3.93|P-value:1.79E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:-4.18|P-value:7.16E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:11.00|P-value:1.21E-23 ID:SGTC_300|Compound:r073-0011|FD-Score:4.21|P-value:6.78E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:5.63|P-value:1.62E-7 ID:SGTC_351|Compound:caspofungin|FD-Score:3.61|P-value:5.23E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:9.27|P-value:1.93E-17 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.68|P-value:4.14E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:7.27|P-value:2.01E-11 ID:SGTC_445|Compound:e6 berbamine|FD-Score:10.10|P-value:3.56E-20 ID:SGTC_488|Compound:nitrendipine|FD-Score:4.19|P-value:7.27E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:8.69|P-value:1.47E-15 ID:SGTC_528|Compound:3492-0019|FD-Score:16.40|P-value:1.27E-50 ID:SGTC_537|Compound:1334-0398|FD-Score:-3.82|P-value:2.57E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:4.73|P-value:8.69E-6 ID:SGTC_841|Compound:0251-0215|FD-Score:10.40|P-value:1.28E-21 ID:SGTC_892|Compound:0929-0076|FD-Score:3.57|P-value:6.05E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:-3.77|P-value:3.02E-4 ID:SGTC_923|Compound:1900-2434|FD-Score:3.43|P-value:9.37E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:-3.72|P-value:3.53E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:-3.41|P-value:9.60E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:3.57|P-value:6.07E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:4.04|P-value:1.23E-4 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:4.12|P-value:9.21E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.93|P-value:1.81E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:-4.01|P-value:1.33E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:4.37|P-value:3.69E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:4.38|P-value:3.51E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:5.47|P-value:3.46E-7 ID:SGTC_1251|Compound:0490-5699|FD-Score:-3.58|P-value:5.63E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:4.42|P-value:3.01E-5 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.63|P-value:4.98E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:4.39|P-value:3.38E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.72|P-value:3.70E-4 ID:SGTC_1383|Compound:3049-0032|FD-Score:-5.95|P-value:3.18E-8 ID:SGTC_1388|Compound:3346-4487|FD-Score:3.61|P-value:5.25E-4 ID:SGTC_1403|Compound:3909-7815|FD-Score:10.40|P-value:2.62E-21 ID:SGTC_1448|Compound:4255-0083|FD-Score:4.10|P-value:1.00E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:4.69|P-value:1.06E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.51|P-value:2.15E-5 ID:SGTC_1581|Compound:himbacine|FD-Score:4.79|P-value:6.79E-6 ID:SGTC_1658|Compound:st012944|FD-Score:4.13|P-value:8.85E-5 ID:SGTC_1687|Compound:st020778|FD-Score:8.31|P-value:2.22E-14 ID:SGTC_1730|Compound:st033808|FD-Score:-3.50|P-value:7.25E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-4.20|P-value:6.67E-5 ID:SGTC_1744|Compound:st037937|FD-Score:-3.50|P-value:7.37E-4 ID:SGTC_1769|Compound:st044406|FD-Score:3.69|P-value:4.05E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.57|P-value:5.96E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:7.45|P-value:6.86E-12 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.48|P-value:2.41E-5 ID:SGTC_1962|Compound:st076595|FD-Score:3.55|P-value:6.50E-4 ID:SGTC_2030|Compound:5175110|FD-Score:5.69|P-value:1.19E-7 ID:SGTC_2046|Compound:4110841|FD-Score:6.08|P-value:1.75E-8 ID:SGTC_2105|Compound:5350118|FD-Score:-3.78|P-value:2.96E-4 ID:SGTC_2291|Compound:7917025|FD-Score:6.19|P-value:9.57E-9 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:4.36|P-value:3.78E-5 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.99|P-value:1.42E-4 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.97|P-value:1.57E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.28|P-value:8.36E-7 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:3.51|P-value:7.35E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.39|P-value:3.36E-5 ID:SGTC_2779|Compound:methotrexate|FD-Score:12.20|P-value:7.94E-29 ID:SGTC_2810|Compound:7959871|FD-Score:3.79|P-value:2.97E-4 ID:SGTC_2923|Compound:7992654|FD-Score:3.84|P-value:2.45E-4 ID:SGTC_2930|Compound:9013230|FD-Score:4.76|P-value:7.96E-6 ID:SGTC_3029|Compound:9088683|FD-Score:3.65|P-value:4.59E-4 ID:SGTC_3141|Compound:9094609|FD-Score:3.48|P-value:7.86E-4 ID:SGTC_3227|Compound:9130768|FD-Score:4.61|P-value:1.45E-5 ID:SGTC_3305|Compound:9127027|FD-Score:-3.60|P-value:5.33E-4

Top fitness defect scores for YBR288C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_528 3492-0019 16.40 172.0 µM 1.27E-50 calcium & mitochondrial duress 7 6.16E-61
2 SGTC_2779 methotrexate 12.20 400.0 µM 7.94E-29 3 1.54E-34
3 SGTC_245 methotrexate 11.00 289.6 µM 1.21E-23 2 2.90E-28
4 SGTC_841 0251-0215 10.40 91.2 µM 1.28E-21 12 8.14E-26
5 SGTC_1403 3909-7815 10.40 62.9 µM 2.62E-21 1 1.94E-25
6 SGTC_445 e6 berbamine 10.10 66.0 µM 3.56E-20 NEO1-PIK1 4 4.56E-24
7 SGTC_363 0455-0021 9.27 3.1 µM 1.93E-17 azole & statin 9 9.22E-21
8 SGTC_524 r073-0010 8.69 27.8 µM 1.47E-15 7 1.74E-18
9 SGTC_1687 st020778 8.31 55.4 µM 2.22E-14 65 4.65E-17
10 SGTC_1873 rapamycin 7.45 2.0 nM 6.86E-12 14 4.76E-14
11 SGTC_391 gliotoxin 7.27 8.3 µM 2.01E-11 14 1.75E-13
12 SGTC_2291 7917025 6.19 104.3 µM 9.57E-9 iron homeostasis 19 2.99E-10
13 SGTC_2046 4110841 6.08 106.0 µM 1.75E-8 iron homeostasis 6 6.19E-10
14 SGTC_2030 5175110 5.69 200.0 µM 1.19E-7 Golgi 7 6.24E-9
15 SGTC_301 4119-0100 5.63 24.5 µM 1.62E-7 NEO1 45 9.09E-9
16 SGTC_1247 0448-0043 5.47 5.8 µM 3.46E-7 ERG2 15 2.26E-8
17 SGTC_2582 agaric acid 5.28 100.0 µM 8.36E-7 redox potentiating 205 6.56E-8
18 SGTC_1581 himbacine 4.79 57.9 µM 6.79E-6 Golgi 33 8.18E-7
19 SGTC_2930 9013230 4.76 14.9 µM 7.96E-6 heme biosynthesis & mitochondrial translocase 17 9.90E-7
20 SGTC_805 3852-0218 4.73 479.0 µM 8.69E-6 ERAD & cell cycle 44 1.10E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.439 8.26E-158 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.437 7.94E-157 YGR261C APL6 hom Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
0.419 8.06E-143 YPL195W APL5 hom Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
0.386 1.37E-119 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.384 2.80E-118 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.331 1.14E-86 YDL077C VAM6 hom Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.293 2.10E-67 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.279 4.77E-61 YML001W YPT7 hom Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
0.263 2.86E-54 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.256 1.87E-51 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.244 1.18E-46 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.234 5.72E-43 YBR131W CCZ1 hom Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway
0.233 1.57E-42 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
0.223 5.23E-39 YGR267C FOL2 het GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
0.221 1.96E-38 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance