YCL024W / KCC4

Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL024W / KCC4

Click on Significant Values for Screen Details ID:SGTC_12|Compound:0849-0099|FD-Score:3.59|P-value:1.39E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:-3.95|P-value:2.96E-5 ID:SGTC_728|Compound:3030-5151|FD-Score:3.23|P-value:5.46E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:3.25|P-value:4.93E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.27|P-value:4.39E-4 ID:SGTC_1407|Compound:3909-7909|FD-Score:5.73|P-value:3.11E-9 ID:SGTC_1419|Compound:3993-2473|FD-Score:-3.13|P-value:7.24E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.67|P-value:1.02E-4 ID:SGTC_1650|Compound:st011916|FD-Score:3.95|P-value:3.12E-5 ID:SGTC_1742|Compound:st038235|FD-Score:6.13|P-value:2.64E-10 ID:SGTC_1781|Compound:st038725|FD-Score:3.19|P-value:6.25E-4 ID:SGTC_1871|Compound:st057448|FD-Score:4.15|P-value:1.30E-5 ID:SGTC_1898|Compound:st060199|FD-Score:4.25|P-value:8.25E-6 ID:SGTC_2002|Compound:4023503|FD-Score:-3.57|P-value:1.43E-4 ID:SGTC_2004|Compound:4023728|FD-Score:3.28|P-value:4.57E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:4.59|P-value:1.62E-6 ID:SGTC_2423|Compound:st077119|FD-Score:3.39|P-value:3.00E-4 ID:SGTC_2460|Compound:5552655|FD-Score:-3.44|P-value:2.36E-4 ID:SGTC_2474|Compound:5321570|FD-Score:4.14|P-value:1.35E-5 ID:SGTC_2764|Compound:methyldopa|FD-Score:4.95|P-value:2.64E-7 ID:SGTC_2772|Compound:vinpocetine|FD-Score:6.60|P-value:1.11E-11 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.18|P-value:6.46E-4 ID:SGTC_2854|Compound:9024559|FD-Score:-3.40|P-value:2.67E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.44|P-value:2.52E-4 ID:SGTC_3205|Compound:9113483|FD-Score:5.07|P-value:1.39E-7 ID:SGTC_3291|Compound:9121177|FD-Score:3.37|P-value:3.27E-4 ID:SGTC_12|Compound:0849-0099|FD-Score:3.59|P-value:1.39E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:-3.95|P-value:2.96E-5 ID:SGTC_728|Compound:3030-5151|FD-Score:3.23|P-value:5.46E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:3.25|P-value:4.93E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.27|P-value:4.39E-4 ID:SGTC_1407|Compound:3909-7909|FD-Score:5.73|P-value:3.11E-9 ID:SGTC_1419|Compound:3993-2473|FD-Score:-3.13|P-value:7.24E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.67|P-value:1.02E-4 ID:SGTC_1650|Compound:st011916|FD-Score:3.95|P-value:3.12E-5 ID:SGTC_1742|Compound:st038235|FD-Score:6.13|P-value:2.64E-10 ID:SGTC_1781|Compound:st038725|FD-Score:3.19|P-value:6.25E-4 ID:SGTC_1871|Compound:st057448|FD-Score:4.15|P-value:1.30E-5 ID:SGTC_1898|Compound:st060199|FD-Score:4.25|P-value:8.25E-6 ID:SGTC_2002|Compound:4023503|FD-Score:-3.57|P-value:1.43E-4 ID:SGTC_2004|Compound:4023728|FD-Score:3.28|P-value:4.57E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:4.59|P-value:1.62E-6 ID:SGTC_2423|Compound:st077119|FD-Score:3.39|P-value:3.00E-4 ID:SGTC_2460|Compound:5552655|FD-Score:-3.44|P-value:2.36E-4 ID:SGTC_2474|Compound:5321570|FD-Score:4.14|P-value:1.35E-5 ID:SGTC_2764|Compound:methyldopa|FD-Score:4.95|P-value:2.64E-7 ID:SGTC_2772|Compound:vinpocetine|FD-Score:6.60|P-value:1.11E-11 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.18|P-value:6.46E-4 ID:SGTC_2854|Compound:9024559|FD-Score:-3.40|P-value:2.67E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.44|P-value:2.52E-4 ID:SGTC_3205|Compound:9113483|FD-Score:5.07|P-value:1.39E-7 ID:SGTC_3291|Compound:9121177|FD-Score:3.37|P-value:3.27E-4

Top fitness defect scores for YCL024W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2772 vinpocetine 6.60 57.1 µM 1.11E-11 3 2.04E-11
2 SGTC_1742 st038235 6.13 28.9 µM 2.64E-10 mitochondrial stress 43 4.45E-10
3 SGTC_1407 3909-7909 5.73 12.3 µM 3.11E-9 60S ribosome export 2 4.91E-9
4 SGTC_3205 9113483 5.07 49.5 µM 1.39E-7 7 1.99E-7
5 SGTC_2764 methyldopa 4.95 94.7 µM 2.64E-7 9 3.70E-7
6 SGTC_2130 NSC 7 4.59 2.5 µM 1.62E-6 copper-dependent oxidative stress 13 2.17E-6
7 SGTC_1898 st060199 4.25 18.6 µM 8.25E-6 8 1.06E-5
8 SGTC_1871 st057448 4.15 67.3 µM 1.30E-5 20 1.64E-5
9 SGTC_2474 5321570 4.14 43.3 µM 1.35E-5 8 1.71E-5
10 SGTC_1650 st011916 3.95 45.8 µM 3.12E-5 15 3.86E-5
11 SGTC_1634 st006576 3.67 41.0 µM 1.02E-4 TSC3-RPN4 28 1.22E-4
12 SGTC_12 0849-0099 3.59 9.3 µM 1.39E-4 29 1.66E-4
13 SGTC_2950 9072895 3.44 21.5 µM 2.52E-4 63 2.96E-4
14 SGTC_2423 st077119 3.39 87.2 µM 3.00E-4 27 3.51E-4
15 SGTC_3291 9121177 3.37 71.4 µM 3.27E-4 26 3.81E-4
16 SGTC_2004 4023728 3.28 41.9 µM 4.57E-4 57 5.28E-4
17 SGTC_944 3370-0224 3.25 231.0 µM 4.93E-4 54 5.68E-4
18 SGTC_728 3030-5151 3.23 22.5 µM 5.46E-4 32 6.28E-4
19 SGTC_1781 st038725 3.19 66.3 µM 6.25E-4 NEO1 147 7.17E-4
20 SGTC_2778 tioconazole 3.18 65.1 nM 6.46E-4 ergosterol depletion effects on membrane 105 7.40E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.123 7.44E-13 YER183C FAU1 hom 5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis
0.121 2.26E-12 YOL028C YAP7 hom Putative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication
0.115 2.20E-11 YIL168W YIL168W hom Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase
0.109 2.69E-10 YPL164C MLH3 hom Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability
0.103 1.97E-9 YER001W MNN1 hom Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family
0.101 4.39E-9 YGL061C DUO1 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
0.100 6.62E-9 YOL147C PEX11 hom Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p
0.096 2.16E-8 YBR204C LDH1 hom Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif)
0.093 6.75E-8 YKL169C_d YKL169C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38
0.089 2.35E-7 YDR527W RBA50 het Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.087 4.43E-7 YKL034W TUL1 hom Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1
0.086 5.40E-7 YJR057W CDC8 het Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
0.086 6.37E-7 YKR027W BCH2 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication
0.085 8.18E-7 YIL093C RSM25 hom Mitochondrial ribosomal protein of the small subunit
0.083 1.68E-6 YJL136C RPS21B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication