YCL035C / GRX1

Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL035C / GRX1

Click on Significant Values for Screen Details ID:SGTC_27|Compound:0485-0001|FD-Score:3.05|P-value:5.52E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.00|P-value:6.75E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.55|P-value:7.36E-5 ID:SGTC_697|Compound:0508-1955|FD-Score:3.54|P-value:7.57E-5 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.38|P-value:1.51E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:-3.21|P-value:3.05E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-4.93|P-value:6.77E-8 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:-3.40|P-value:1.37E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.03|P-value:5.86E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-7.57|P-value:2.95E-16 ID:SGTC_1109|Compound:tnp00067|FD-Score:2.92|P-value:8.82E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.93|P-value:8.52E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-2.95|P-value:8.19E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:3.08|P-value:4.79E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:3.10|P-value:4.59E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:-2.94|P-value:8.31E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.21|P-value:3.34E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.08|P-value:6.43E-6 ID:SGTC_2253|Compound:7938677|FD-Score:3.02|P-value:6.10E-4 ID:SGTC_2349|Compound:9023730|FD-Score:3.99|P-value:9.87E-6 ID:SGTC_2359|Compound:9039622|FD-Score:3.72|P-value:3.42E-5 ID:SGTC_2397|Compound:5161689|FD-Score:-3.17|P-value:3.54E-4 ID:SGTC_2403|Compound:6032726|FD-Score:3.16|P-value:3.58E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.51|P-value:8.59E-5 ID:SGTC_2413|Compound:inermin|FD-Score:4.22|P-value:3.09E-6 ID:SGTC_2419|Compound:paclitaxel|FD-Score:2.89|P-value:9.69E-4 ID:SGTC_2468|Compound:5987765|FD-Score:2.92|P-value:8.81E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.23|P-value:2.73E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.02|P-value:6.15E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:2.92|P-value:8.97E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:2.93|P-value:8.60E-4 ID:SGTC_2771|Compound:fluoxetine|FD-Score:3.23|P-value:2.69E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-3.12|P-value:4.21E-4 ID:SGTC_2862|Compound:9027634|FD-Score:-4.49|P-value:7.95E-7 ID:SGTC_2868|Compound:9035608|FD-Score:4.17|P-value:4.01E-6 ID:SGTC_2874|Compound:9043904|FD-Score:-3.60|P-value:5.85E-5 ID:SGTC_2876|Compound:9059903|FD-Score:5.54|P-value:1.48E-9 ID:SGTC_2909|Compound:7941780|FD-Score:4.46|P-value:9.08E-7 ID:SGTC_2978|Compound:9011784|FD-Score:3.69|P-value:3.92E-5 ID:SGTC_3067|Compound:9117431|FD-Score:-3.01|P-value:6.40E-4 ID:SGTC_3165|Compound:9101544|FD-Score:2.98|P-value:7.05E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-3.81|P-value:2.27E-5 ID:SGTC_27|Compound:0485-0001|FD-Score:3.05|P-value:5.52E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.00|P-value:6.75E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.55|P-value:7.36E-5 ID:SGTC_697|Compound:0508-1955|FD-Score:3.54|P-value:7.57E-5 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.38|P-value:1.51E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:-3.21|P-value:3.05E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:-4.93|P-value:6.77E-8 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:-3.40|P-value:1.37E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.03|P-value:5.86E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-7.57|P-value:2.95E-16 ID:SGTC_1109|Compound:tnp00067|FD-Score:2.92|P-value:8.82E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.93|P-value:8.52E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-2.95|P-value:8.19E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:3.08|P-value:4.79E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:3.10|P-value:4.59E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:-2.94|P-value:8.31E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.21|P-value:3.34E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.08|P-value:6.43E-6 ID:SGTC_2253|Compound:7938677|FD-Score:3.02|P-value:6.10E-4 ID:SGTC_2349|Compound:9023730|FD-Score:3.99|P-value:9.87E-6 ID:SGTC_2359|Compound:9039622|FD-Score:3.72|P-value:3.42E-5 ID:SGTC_2397|Compound:5161689|FD-Score:-3.17|P-value:3.54E-4 ID:SGTC_2403|Compound:6032726|FD-Score:3.16|P-value:3.58E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.51|P-value:8.59E-5 ID:SGTC_2413|Compound:inermin|FD-Score:4.22|P-value:3.09E-6 ID:SGTC_2419|Compound:paclitaxel|FD-Score:2.89|P-value:9.69E-4 ID:SGTC_2468|Compound:5987765|FD-Score:2.92|P-value:8.81E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.23|P-value:2.73E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.02|P-value:6.15E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:2.92|P-value:8.97E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:2.93|P-value:8.60E-4 ID:SGTC_2771|Compound:fluoxetine|FD-Score:3.23|P-value:2.69E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-3.12|P-value:4.21E-4 ID:SGTC_2862|Compound:9027634|FD-Score:-4.49|P-value:7.95E-7 ID:SGTC_2868|Compound:9035608|FD-Score:4.17|P-value:4.01E-6 ID:SGTC_2874|Compound:9043904|FD-Score:-3.60|P-value:5.85E-5 ID:SGTC_2876|Compound:9059903|FD-Score:5.54|P-value:1.48E-9 ID:SGTC_2909|Compound:7941780|FD-Score:4.46|P-value:9.08E-7 ID:SGTC_2978|Compound:9011784|FD-Score:3.69|P-value:3.92E-5 ID:SGTC_3067|Compound:9117431|FD-Score:-3.01|P-value:6.40E-4 ID:SGTC_3165|Compound:9101544|FD-Score:2.98|P-value:7.05E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-3.81|P-value:2.27E-5

Top fitness defect scores for YCL035C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2876 9059903 5.54 26.0 µM 1.48E-9 2 1.48E-8
2 SGTC_2909 7941780 4.46 10.5 µM 9.08E-7 10 4.12E-6
3 SGTC_2413 inermin 4.22 26.1 µM 3.09E-6 43 1.21E-5
4 SGTC_2868 9035608 4.17 32.5 µM 4.01E-6 14 1.52E-5
5 SGTC_2349 9023730 3.99 187.1 µM 9.87E-6 22 3.34E-5
6 SGTC_2359 9039622 3.72 200.0 µM 3.42E-5 endomembrane recycling 27 9.98E-5
7 SGTC_2978 9011784 3.69 43.0 µM 3.92E-5 39 1.12E-4
8 SGTC_697 0508-1955 3.54 564.0 µM 7.57E-5 53 2.01E-4
9 SGTC_2408 sulbentine 3.51 18.4 µM 8.59E-5 64 2.24E-4
10 SGTC_2771 fluoxetine 3.23 41.0 µM 2.69E-4 40 6.12E-4
11 SGTC_2403 6032726 3.16 181.2 µM 3.58E-4 73 7.88E-4
12 SGTC_1234 0326-0494 3.10 15.5 µM 4.59E-4 42 9.81E-4
13 SGTC_1148 3909-7663 3.08 27.7 µM 4.79E-4 47 0.00102
14 SGTC_27 0485-0001 3.05 32.6 µM 5.52E-4 RPP1 & pyrimidine depletion 24 0.00115
15 SGTC_1059 3448-6867 3.03 171.0 µM 5.86E-4 43 0.00122
16 SGTC_2253 7938677 3.02 135.8 µM 6.10E-4 44 0.00126
17 SGTC_3165 9101544 2.98 49.5 µM 7.05E-4 39 0.00143
18 SGTC_1125 tnp00248 2.93 1.3 µM 8.52E-4 108 0.00169
19 SGTC_2767 sibutramine 2.93 71.5 µM 8.60E-4 66 0.00171
20 SGTC_2468 5987765 2.92 72.9 µM 8.81E-4 56 0.00174

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.239 1.13E-44 YBL086C_p YBL086C_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.238 2.73E-44 YOR238W_p YOR238W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.232 3.57E-42 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.229 2.63E-41 YMR150C IMP1 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.220 5.87E-38 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.219 1.15E-37 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.209 1.58E-34 YDR508C GNP1 hom High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication
0.202 3.31E-32 YDR242W AMD2 hom Putative amidase
0.200 1.70E-31 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.199 2.24E-31 YER060W-A FCY22 hom Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.193 1.27E-29 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.193 1.45E-29 YBR262C AIM5 hom Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.191 8.46E-29 YOR012W_p YOR012W_p hom Putative protein of unknown function
0.189 1.85E-28 YBL015W ACH1 hom Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
0.188 4.56E-28 YGR097W ASK10 hom Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication