YCL050C / APA1

AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL050C / APA1

Click on Significant Values for Screen Details ID:SGTC_147|Compound:4466-0538|FD-Score:-3.78|P-value:4.56E-5 ID:SGTC_334|Compound:1502-1024|FD-Score:3.20|P-value:5.47E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:3.19|P-value:5.61E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.06|P-value:7.47E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:-3.39|P-value:2.20E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:3.29|P-value:3.86E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:-3.56|P-value:1.15E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:3.14|P-value:6.73E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:3.27|P-value:4.27E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:4.43|P-value:2.85E-6 ID:SGTC_1074|Compound:carmofur|FD-Score:3.06|P-value:8.91E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:-3.87|P-value:3.09E-5 ID:SGTC_1396|Compound:3511-0007|FD-Score:6.71|P-value:2.98E-12 ID:SGTC_1467|Compound:k284-1082|FD-Score:-3.39|P-value:2.25E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:4.37|P-value:3.95E-6 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:3.11|P-value:7.55E-4 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.38|P-value:2.80E-4 ID:SGTC_1782|Compound:st039423|FD-Score:3.35|P-value:3.07E-4 ID:SGTC_1791|Compound:st048313|FD-Score:3.97|P-value:2.45E-5 ID:SGTC_1828|Compound:st055811|FD-Score:3.33|P-value:3.37E-4 ID:SGTC_1833|Compound:st055984|FD-Score:3.14|P-value:6.76E-4 ID:SGTC_1871|Compound:st057448|FD-Score:3.13|P-value:7.09E-4 ID:SGTC_1912|Compound:st060209|FD-Score:4.45|P-value:2.58E-6 ID:SGTC_2135|Compound:5326629|FD-Score:4.33|P-value:4.62E-6 ID:SGTC_2268|Compound:7933382|FD-Score:3.18|P-value:5.77E-4 ID:SGTC_2273|Compound:7815444|FD-Score:3.95|P-value:2.65E-5 ID:SGTC_2317|Compound:5986382|FD-Score:3.47|P-value:1.94E-4 ID:SGTC_2319|Compound:6033012|FD-Score:3.14|P-value:6.61E-4 ID:SGTC_2327|Compound:6189656|FD-Score:-3.25|P-value:3.77E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.48|P-value:1.88E-4 ID:SGTC_2480|Compound:5767542|FD-Score:3.38|P-value:2.74E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:3.25|P-value:4.49E-4 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.23|P-value:4.79E-4 ID:SGTC_2699|Compound:st077704|FD-Score:3.08|P-value:8.21E-4 ID:SGTC_2912|Compound:7964298|FD-Score:3.27|P-value:4.26E-4 ID:SGTC_2947|Compound:9072639|FD-Score:3.05|P-value:9.20E-4 ID:SGTC_2963|Compound:9082507|FD-Score:6.15|P-value:1.44E-10 ID:SGTC_2966|Compound:9084041|FD-Score:4.24|P-value:7.13E-6 ID:SGTC_3086|Compound:9117350|FD-Score:-3.02|P-value:8.85E-4 ID:SGTC_3103|Compound:9120822|FD-Score:-3.17|P-value:5.15E-4 ID:SGTC_3118|Compound:9124250|FD-Score:-3.05|P-value:7.96E-4 ID:SGTC_3201|Compound:9113999|FD-Score:5.45|P-value:1.22E-8 ID:SGTC_3204|Compound:9114183|FD-Score:-3.28|P-value:3.42E-4 ID:SGTC_3207|Compound:9114163|FD-Score:-4.12|P-value:1.02E-5 ID:SGTC_3209|Compound:9113616|FD-Score:3.17|P-value:6.12E-4 ID:SGTC_3282|Compound:9108498|FD-Score:3.14|P-value:6.81E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:-3.78|P-value:4.56E-5 ID:SGTC_334|Compound:1502-1024|FD-Score:3.20|P-value:5.47E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:3.19|P-value:5.61E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.06|P-value:7.47E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:-3.39|P-value:2.20E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:3.29|P-value:3.86E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:-3.56|P-value:1.15E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:3.14|P-value:6.73E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:3.27|P-value:4.27E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:4.43|P-value:2.85E-6 ID:SGTC_1074|Compound:carmofur|FD-Score:3.06|P-value:8.91E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:-3.87|P-value:3.09E-5 ID:SGTC_1396|Compound:3511-0007|FD-Score:6.71|P-value:2.98E-12 ID:SGTC_1467|Compound:k284-1082|FD-Score:-3.39|P-value:2.25E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:4.37|P-value:3.95E-6 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:3.11|P-value:7.55E-4 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.38|P-value:2.80E-4 ID:SGTC_1782|Compound:st039423|FD-Score:3.35|P-value:3.07E-4 ID:SGTC_1791|Compound:st048313|FD-Score:3.97|P-value:2.45E-5 ID:SGTC_1828|Compound:st055811|FD-Score:3.33|P-value:3.37E-4 ID:SGTC_1833|Compound:st055984|FD-Score:3.14|P-value:6.76E-4 ID:SGTC_1871|Compound:st057448|FD-Score:3.13|P-value:7.09E-4 ID:SGTC_1912|Compound:st060209|FD-Score:4.45|P-value:2.58E-6 ID:SGTC_2135|Compound:5326629|FD-Score:4.33|P-value:4.62E-6 ID:SGTC_2268|Compound:7933382|FD-Score:3.18|P-value:5.77E-4 ID:SGTC_2273|Compound:7815444|FD-Score:3.95|P-value:2.65E-5 ID:SGTC_2317|Compound:5986382|FD-Score:3.47|P-value:1.94E-4 ID:SGTC_2319|Compound:6033012|FD-Score:3.14|P-value:6.61E-4 ID:SGTC_2327|Compound:6189656|FD-Score:-3.25|P-value:3.77E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.48|P-value:1.88E-4 ID:SGTC_2480|Compound:5767542|FD-Score:3.38|P-value:2.74E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:3.25|P-value:4.49E-4 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.23|P-value:4.79E-4 ID:SGTC_2699|Compound:st077704|FD-Score:3.08|P-value:8.21E-4 ID:SGTC_2912|Compound:7964298|FD-Score:3.27|P-value:4.26E-4 ID:SGTC_2947|Compound:9072639|FD-Score:3.05|P-value:9.20E-4 ID:SGTC_2963|Compound:9082507|FD-Score:6.15|P-value:1.44E-10 ID:SGTC_2966|Compound:9084041|FD-Score:4.24|P-value:7.13E-6 ID:SGTC_3086|Compound:9117350|FD-Score:-3.02|P-value:8.85E-4 ID:SGTC_3103|Compound:9120822|FD-Score:-3.17|P-value:5.15E-4 ID:SGTC_3118|Compound:9124250|FD-Score:-3.05|P-value:7.96E-4 ID:SGTC_3201|Compound:9113999|FD-Score:5.45|P-value:1.22E-8 ID:SGTC_3204|Compound:9114183|FD-Score:-3.28|P-value:3.42E-4 ID:SGTC_3207|Compound:9114163|FD-Score:-4.12|P-value:1.02E-5 ID:SGTC_3209|Compound:9113616|FD-Score:3.17|P-value:6.12E-4 ID:SGTC_3282|Compound:9108498|FD-Score:3.14|P-value:6.81E-4

Top fitness defect scores for YCL050C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1396 3511-0007 6.71 98.6 µM 2.98E-12 mitochondrial processes 1 9.58E-12
2 SGTC_2963 9082507 6.15 9.0 µM 1.44E-10 5 3.80E-10
3 SGTC_3201 9113999 5.45 49.5 µM 1.22E-8 8 2.59E-8
4 SGTC_1912 st060209 4.45 90.8 µM 2.58E-6 28 4.21E-6
5 SGTC_1011 4112-3381 4.43 17.4 µM 2.85E-6 27 4.62E-6
6 SGTC_1473 k844-0725 4.37 134.0 µM 3.95E-6 6 6.31E-6
7 SGTC_2135 5326629 4.33 150.4 µM 4.62E-6 RPP1 & pyrimidine depletion 18 7.33E-6
8 SGTC_2966 9084041 4.24 48.0 µM 7.13E-6 25 1.11E-5
9 SGTC_1791 st048313 3.97 56.1 µM 2.45E-5 13 3.59E-5
10 SGTC_2273 7815444 3.95 169.1 µM 2.65E-5 21 3.86E-5
11 SGTC_2410 aloe-emodin-8-o-glycoside 3.48 46.3 µM 1.88E-4 mitochondrial processes 48 2.50E-4
12 SGTC_2317 5986382 3.47 200.0 µM 1.94E-4 24 2.58E-4
13 SGTC_2480 5767542 3.38 37.2 µM 2.74E-4 24 3.59E-4
14 SGTC_1587 estradiol valerate 3.38 56.1 µM 2.80E-4 26 3.65E-4
15 SGTC_1782 st039423 3.35 80.6 µM 3.07E-4 40 4.00E-4
16 SGTC_1828 st055811 3.33 49.0 µM 3.37E-4 18 4.36E-4
17 SGTC_534 k292-0788 3.29 63.4 µM 3.86E-4 mitochondrial processes 50 4.97E-4
18 SGTC_2912 7964298 3.27 10.9 µM 4.26E-4 cell wall 125 5.46E-4
19 SGTC_879 0831-1030 3.27 162.0 µM 4.27E-4 83 5.47E-4
20 SGTC_2614 arthonioic acid 3.25 13.4 µM 4.49E-4 44 5.75E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.091 1.36E-7 YPL192C PRM3 hom Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body
0.085 7.16E-7 YPL206C PGC1 hom Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs
0.085 8.24E-7 YCL028W RNQ1 hom [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate
0.084 1.12E-6 YOL010W RCL1 het Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected
0.084 1.20E-6 YMR246W FAA4 hom Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication
0.079 4.22E-6 YBR104W YMC2 hom Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication
0.075 1.19E-5 YPL086C ELP3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin
0.072 2.77E-5 YOR024W_d YOR024W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.071 3.41E-5 YHR069C RRP4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
0.071 3.89E-5 YMR065W KAR5 hom Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone
0.071 4.02E-5 YPL184C MRN1 hom RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation
0.070 4.76E-5 YMR222C FSH2 hom Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2
0.070 5.32E-5 YDR219C MFB1 hom Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding
0.068 8.66E-5 YHR177W_p YHR177W_p hom Putative protein of unknown function; overexpression causes a cell cycle delay or arrest
0.068 8.86E-5 YMR086C-A_d YMR086C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data