YCL051W / LRE1

Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway;
LRE1 has a paralog, HLR1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL051W / LRE1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:9.12|P-value:1.85E-18 ID:SGTC_121|Compound:0557-0495|FD-Score:3.50|P-value:4.45E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:3.30|P-value:8.71E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:3.64|P-value:2.73E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:-3.84|P-value:1.17E-4 ID:SGTC_715|Compound:0868-0130|FD-Score:5.41|P-value:1.34E-7 ID:SGTC_731|Compound:3966-0296|FD-Score:3.63|P-value:2.80E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:4.15|P-value:4.01E-5 ID:SGTC_835|Compound:0312-0010|FD-Score:3.38|P-value:6.66E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:3.96|P-value:8.33E-5 ID:SGTC_907|Compound:3448-6986|FD-Score:4.04|P-value:6.15E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:3.29|P-value:8.99E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:4.43|P-value:1.28E-5 ID:SGTC_1064|Compound:honokiol|FD-Score:3.38|P-value:6.59E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:4.61|P-value:5.89E-6 ID:SGTC_1134|Compound:3477-0009|FD-Score:5.19|P-value:3.87E-7 ID:SGTC_1263|Compound:0774-1208|FD-Score:4.64|P-value:5.03E-6 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.23|P-value:9.87E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:4.05|P-value:5.96E-5 ID:SGTC_1442|Compound:4112-3290|FD-Score:3.86|P-value:1.23E-4 ID:SGTC_1846|Compound:st056244|FD-Score:4.16|P-value:3.80E-5 ID:SGTC_1853|Compound:st056710|FD-Score:3.59|P-value:3.24E-4 ID:SGTC_1871|Compound:st057448|FD-Score:3.51|P-value:4.32E-4 ID:SGTC_1908|Compound:st060353|FD-Score:3.94|P-value:8.88E-5 ID:SGTC_1958|Compound:st076513|FD-Score:4.79|P-value:2.58E-6 ID:SGTC_1969|Compound:st069479|FD-Score:3.26|P-value:9.69E-4 ID:SGTC_1983|Compound:st071813|FD-Score:7.08|P-value:7.67E-12 ID:SGTC_2003|Compound:4023581|FD-Score:3.35|P-value:7.35E-4 ID:SGTC_2008|Compound:4012253|FD-Score:4.50|P-value:9.37E-6 ID:SGTC_2060|Compound:5238662|FD-Score:3.45|P-value:5.23E-4 ID:SGTC_2251|Compound:lansoprazole|FD-Score:3.87|P-value:1.17E-4 ID:SGTC_2268|Compound:7933382|FD-Score:4.13|P-value:4.38E-5 ID:SGTC_2336|Compound:9001819|FD-Score:-3.53|P-value:3.60E-4 ID:SGTC_2337|Compound:9009472|FD-Score:-3.80|P-value:1.36E-4 ID:SGTC_2585|Compound:alliin|FD-Score:3.47|P-value:4.95E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:4.10|P-value:4.88E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:5.47|P-value:9.84E-8 ID:SGTC_2887|Compound:9044784|FD-Score:-3.44|P-value:4.82E-4 ID:SGTC_3016|Compound:9080866|FD-Score:4.27|P-value:2.41E-5 ID:SGTC_3091|Compound:9116372|FD-Score:3.67|P-value:2.46E-4 ID:SGTC_3195|Compound:9113082|FD-Score:4.37|P-value:1.60E-5 ID:SGTC_3225|Compound:9130819|FD-Score:3.50|P-value:4.49E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:9.12|P-value:1.85E-18 ID:SGTC_121|Compound:0557-0495|FD-Score:3.50|P-value:4.45E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:3.30|P-value:8.71E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:3.64|P-value:2.73E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:-3.84|P-value:1.17E-4 ID:SGTC_715|Compound:0868-0130|FD-Score:5.41|P-value:1.34E-7 ID:SGTC_731|Compound:3966-0296|FD-Score:3.63|P-value:2.80E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:4.15|P-value:4.01E-5 ID:SGTC_835|Compound:0312-0010|FD-Score:3.38|P-value:6.66E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:3.96|P-value:8.33E-5 ID:SGTC_907|Compound:3448-6986|FD-Score:4.04|P-value:6.15E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:3.29|P-value:8.99E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:4.43|P-value:1.28E-5 ID:SGTC_1064|Compound:honokiol|FD-Score:3.38|P-value:6.59E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:4.61|P-value:5.89E-6 ID:SGTC_1134|Compound:3477-0009|FD-Score:5.19|P-value:3.87E-7 ID:SGTC_1263|Compound:0774-1208|FD-Score:4.64|P-value:5.03E-6 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.23|P-value:9.87E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:4.05|P-value:5.96E-5 ID:SGTC_1442|Compound:4112-3290|FD-Score:3.86|P-value:1.23E-4 ID:SGTC_1846|Compound:st056244|FD-Score:4.16|P-value:3.80E-5 ID:SGTC_1853|Compound:st056710|FD-Score:3.59|P-value:3.24E-4 ID:SGTC_1871|Compound:st057448|FD-Score:3.51|P-value:4.32E-4 ID:SGTC_1908|Compound:st060353|FD-Score:3.94|P-value:8.88E-5 ID:SGTC_1958|Compound:st076513|FD-Score:4.79|P-value:2.58E-6 ID:SGTC_1969|Compound:st069479|FD-Score:3.26|P-value:9.69E-4 ID:SGTC_1983|Compound:st071813|FD-Score:7.08|P-value:7.67E-12 ID:SGTC_2003|Compound:4023581|FD-Score:3.35|P-value:7.35E-4 ID:SGTC_2008|Compound:4012253|FD-Score:4.50|P-value:9.37E-6 ID:SGTC_2060|Compound:5238662|FD-Score:3.45|P-value:5.23E-4 ID:SGTC_2251|Compound:lansoprazole|FD-Score:3.87|P-value:1.17E-4 ID:SGTC_2268|Compound:7933382|FD-Score:4.13|P-value:4.38E-5 ID:SGTC_2336|Compound:9001819|FD-Score:-3.53|P-value:3.60E-4 ID:SGTC_2337|Compound:9009472|FD-Score:-3.80|P-value:1.36E-4 ID:SGTC_2585|Compound:alliin|FD-Score:3.47|P-value:4.95E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:4.10|P-value:4.88E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:5.47|P-value:9.84E-8 ID:SGTC_2887|Compound:9044784|FD-Score:-3.44|P-value:4.82E-4 ID:SGTC_3016|Compound:9080866|FD-Score:4.27|P-value:2.41E-5 ID:SGTC_3091|Compound:9116372|FD-Score:3.67|P-value:2.46E-4 ID:SGTC_3195|Compound:9113082|FD-Score:4.37|P-value:1.60E-5 ID:SGTC_3225|Compound:9130819|FD-Score:3.50|P-value:4.49E-4

Top fitness defect scores for YCL051W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_16 erodoxin 9.12 22.8 µM 1.85E-18 unfolded protein response 12 3.75E-20
2 SGTC_1983 st071813 7.08 46.9 µM 7.67E-12 RPP1 & pyrimidine depletion 1 7.02E-13
3 SGTC_2690 usnic acid 5.47 100.0 µM 9.84E-8 9 2.28E-8
4 SGTC_715 0868-0130 5.41 184.0 µM 1.34E-7 2 3.22E-8
5 SGTC_1134 3477-0009 5.19 42.5 µM 3.87E-7 19 1.03E-7
6 SGTC_1958 st076513 4.79 41.9 µM 2.58E-6 60S ribosome export 17 8.26E-7
7 SGTC_1263 0774-1208 4.64 64.8 µM 5.03E-6 29 1.72E-6
8 SGTC_1126 isoteolin 4.61 1.0 µM 5.89E-6 15 2.04E-6
9 SGTC_2008 4012253 4.50 172.0 µM 9.37E-6 13 3.40E-6
10 SGTC_933 2914-0560 4.43 114.0 µM 1.28E-5 19 4.77E-6
11 SGTC_3195 9113082 4.37 49.5 µM 1.60E-5 11 6.11E-6
12 SGTC_3016 9080866 4.27 49.5 µM 2.41E-5 21 9.58E-6
13 SGTC_1846 st056244 4.16 14.8 µM 3.80E-5 16 1.58E-5
14 SGTC_734 2023-0417 4.15 80.0 µM 4.01E-5 ergosterol biosynthesis 12 1.67E-5
15 SGTC_2268 7933382 4.13 153.8 µM 4.38E-5 34 1.84E-5
16 SGTC_2617 dihydrotanshinone i 4.10 20.8 µM 4.88E-5 copper-dependent oxidative stress 40 2.08E-5
17 SGTC_1417 3966-0327 4.05 90.4 µM 5.96E-5 15 2.58E-5
18 SGTC_907 3448-6986 4.04 75.5 µM 6.15E-5 25 2.67E-5
19 SGTC_840 0245-0196 3.96 390.0 µM 8.33E-5 13 3.73E-5
20 SGTC_1908 st060353 3.94 56.5 µM 8.88E-5 RPP1 & pyrimidine depletion 36 4.00E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.210 9.19E-35 YPL244C HUT1 hom Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.192 3.74E-29 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.152 8.47E-19 YDL045C FAD1 het Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.132 1.72E-14 YMR161W HLJ1 hom Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.117 8.47E-12 YFL031W HAC1 hom Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.112 8.12E-11 YPL243W SRP68 het Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.110 1.64E-10 YBR229C ROT2 hom Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.105 1.25E-9 YHL020C OPI1 hom Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance
0.096 2.55E-8 YML105C SEC65 het Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
0.095 3.83E-8 YFL041W-A_p YFL041W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.091 1.41E-7 YCL052C PBN1 het Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
0.090 1.57E-7 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.086 6.71E-7 YPR111W DBF20 hom Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs
0.084 1.05E-6 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.080 3.59E-6 YCL012C YCL012C hom Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene