YCL052C / PBN1

Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X

Zygosity: Heterozygous strain
fixedexpanded
Profile for YCL052C / PBN1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:10.00|P-value:9.14E-23 ID:SGTC_35|Compound:0133-0054|FD-Score:4.13|P-value:3.00E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:4.18|P-value:2.38E-5 ID:SGTC_82|Compound:0054-0107|FD-Score:-3.96|P-value:6.34E-5 ID:SGTC_93|Compound:0109-0045|FD-Score:3.54|P-value:2.92E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.89|P-value:7.59E-5 ID:SGTC_100|Compound:0086-0128|FD-Score:7.08|P-value:2.93E-12 ID:SGTC_200|Compound:k038-0079|FD-Score:3.23|P-value:8.32E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:3.90|P-value:7.34E-5 ID:SGTC_579|Compound:4491-1009|FD-Score:4.92|P-value:8.81E-7 ID:SGTC_679|Compound:0422-0091|FD-Score:4.53|P-value:5.33E-6 ID:SGTC_686|Compound:1319-0111|FD-Score:3.19|P-value:9.59E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.80|P-value:1.08E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.19|P-value:9.70E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.30|P-value:1.36E-7 ID:SGTC_855|Compound:0428-0020|FD-Score:3.93|P-value:6.55E-5 ID:SGTC_860|Compound:0443-0016|FD-Score:3.30|P-value:6.71E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:4.42|P-value:8.71E-6 ID:SGTC_1264|Compound:0783-0040|FD-Score:4.53|P-value:5.35E-6 ID:SGTC_1307|Compound:1187-1599|FD-Score:-4.98|P-value:6.84E-7 ID:SGTC_1308|Compound:1187-1617|FD-Score:-3.24|P-value:8.55E-4 ID:SGTC_1312|Compound:1218-2183|FD-Score:-3.34|P-value:6.07E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:-5.28|P-value:1.56E-7 ID:SGTC_1609|Compound:st001574|FD-Score:3.74|P-value:1.36E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.71|P-value:1.55E-4 ID:SGTC_2026|Compound:5148700|FD-Score:4.86|P-value:1.17E-6 ID:SGTC_2112|Compound:5488546|FD-Score:3.28|P-value:7.09E-4 ID:SGTC_2238|Compound:6671620|FD-Score:3.18|P-value:9.99E-4 ID:SGTC_2266|Compound:7929956|FD-Score:3.86|P-value:8.63E-5 ID:SGTC_2290|Compound:7904846|FD-Score:-4.42|P-value:9.22E-6 ID:SGTC_2331|Compound:7980721|FD-Score:6.40|P-value:2.48E-10 ID:SGTC_2335|Compound:9018011|FD-Score:-4.06|P-value:4.24E-5 ID:SGTC_2374|Compound:9071832|FD-Score:-3.20|P-value:9.62E-4 ID:SGTC_2390|Compound:9097792|FD-Score:3.59|P-value:2.44E-4 ID:SGTC_2508|Compound:celastrol|FD-Score:3.38|P-value:5.03E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:-3.29|P-value:7.21E-4 ID:SGTC_2525|Compound:harmine|FD-Score:-3.20|P-value:9.56E-4 ID:SGTC_2601|Compound:securinine|FD-Score:3.18|P-value:9.82E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.37|P-value:5.22E-4 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:3.20|P-value:9.16E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.52|P-value:3.13E-4 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:3.19|P-value:9.69E-4 ID:SGTC_2681|Compound:menadione|FD-Score:4.13|P-value:2.94E-5 ID:SGTC_2709|Compound:st078560|FD-Score:3.42|P-value:4.36E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:-3.33|P-value:6.19E-4 ID:SGTC_2729|Compound:fluphenazine|FD-Score:3.30|P-value:6.59E-4 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:3.29|P-value:6.87E-4 ID:SGTC_2829|Compound:7992743|FD-Score:3.78|P-value:1.19E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.60|P-value:2.28E-4 ID:SGTC_3170|Compound:9103054|FD-Score:-3.48|P-value:3.70E-4 ID:SGTC_3173|Compound:9105693|FD-Score:3.95|P-value:6.06E-5 ID:SGTC_3202|Compound:9113761|FD-Score:3.33|P-value:5.92E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.51|P-value:3.15E-4 ID:SGTC_3211|Compound:9128781|FD-Score:5.72|P-value:1.35E-8 ID:SGTC_3232|Compound:9132874|FD-Score:3.62|P-value:2.18E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:10.00|P-value:9.14E-23 ID:SGTC_35|Compound:0133-0054|FD-Score:4.13|P-value:3.00E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:4.18|P-value:2.38E-5 ID:SGTC_82|Compound:0054-0107|FD-Score:-3.96|P-value:6.34E-5 ID:SGTC_93|Compound:0109-0045|FD-Score:3.54|P-value:2.92E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.89|P-value:7.59E-5 ID:SGTC_100|Compound:0086-0128|FD-Score:7.08|P-value:2.93E-12 ID:SGTC_200|Compound:k038-0079|FD-Score:3.23|P-value:8.32E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:3.90|P-value:7.34E-5 ID:SGTC_579|Compound:4491-1009|FD-Score:4.92|P-value:8.81E-7 ID:SGTC_679|Compound:0422-0091|FD-Score:4.53|P-value:5.33E-6 ID:SGTC_686|Compound:1319-0111|FD-Score:3.19|P-value:9.59E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.80|P-value:1.08E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.19|P-value:9.70E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.30|P-value:1.36E-7 ID:SGTC_855|Compound:0428-0020|FD-Score:3.93|P-value:6.55E-5 ID:SGTC_860|Compound:0443-0016|FD-Score:3.30|P-value:6.71E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:4.42|P-value:8.71E-6 ID:SGTC_1264|Compound:0783-0040|FD-Score:4.53|P-value:5.35E-6 ID:SGTC_1307|Compound:1187-1599|FD-Score:-4.98|P-value:6.84E-7 ID:SGTC_1308|Compound:1187-1617|FD-Score:-3.24|P-value:8.55E-4 ID:SGTC_1312|Compound:1218-2183|FD-Score:-3.34|P-value:6.07E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:-5.28|P-value:1.56E-7 ID:SGTC_1609|Compound:st001574|FD-Score:3.74|P-value:1.36E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.71|P-value:1.55E-4 ID:SGTC_2026|Compound:5148700|FD-Score:4.86|P-value:1.17E-6 ID:SGTC_2112|Compound:5488546|FD-Score:3.28|P-value:7.09E-4 ID:SGTC_2238|Compound:6671620|FD-Score:3.18|P-value:9.99E-4 ID:SGTC_2266|Compound:7929956|FD-Score:3.86|P-value:8.63E-5 ID:SGTC_2290|Compound:7904846|FD-Score:-4.42|P-value:9.22E-6 ID:SGTC_2331|Compound:7980721|FD-Score:6.40|P-value:2.48E-10 ID:SGTC_2335|Compound:9018011|FD-Score:-4.06|P-value:4.24E-5 ID:SGTC_2374|Compound:9071832|FD-Score:-3.20|P-value:9.62E-4 ID:SGTC_2390|Compound:9097792|FD-Score:3.59|P-value:2.44E-4 ID:SGTC_2508|Compound:celastrol|FD-Score:3.38|P-value:5.03E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:-3.29|P-value:7.21E-4 ID:SGTC_2525|Compound:harmine|FD-Score:-3.20|P-value:9.56E-4 ID:SGTC_2601|Compound:securinine|FD-Score:3.18|P-value:9.82E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.37|P-value:5.22E-4 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:3.20|P-value:9.16E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.52|P-value:3.13E-4 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:3.19|P-value:9.69E-4 ID:SGTC_2681|Compound:menadione|FD-Score:4.13|P-value:2.94E-5 ID:SGTC_2709|Compound:st078560|FD-Score:3.42|P-value:4.36E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:-3.33|P-value:6.19E-4 ID:SGTC_2729|Compound:fluphenazine|FD-Score:3.30|P-value:6.59E-4 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:3.29|P-value:6.87E-4 ID:SGTC_2829|Compound:7992743|FD-Score:3.78|P-value:1.19E-4 ID:SGTC_3049|Compound:9092223|FD-Score:3.60|P-value:2.28E-4 ID:SGTC_3170|Compound:9103054|FD-Score:-3.48|P-value:3.70E-4 ID:SGTC_3173|Compound:9105693|FD-Score:3.95|P-value:6.06E-5 ID:SGTC_3202|Compound:9113761|FD-Score:3.33|P-value:5.92E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.51|P-value:3.15E-4 ID:SGTC_3211|Compound:9128781|FD-Score:5.72|P-value:1.35E-8 ID:SGTC_3232|Compound:9132874|FD-Score:3.62|P-value:2.18E-4

Top fitness defect scores for YCL052C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_16 erodoxin 10.00 22.8 µM 9.14E-23 unfolded protein response 4 5.23E-24 2.29
2 SGTC_100 0086-0128 7.08 49.6 µM 2.93E-12 copper-dependent oxidative stress 2 7.06E-13 1.71
3 SGTC_2331 7980721 6.40 200.0 µM 2.48E-10 2 7.74E-11 0.22
4 SGTC_3211 9128781 5.72 49.5 µM 1.35E-8 6 5.35E-9 0.70
5 SGTC_579 4491-1009 4.92 110.0 µM 8.81E-7 60S ribosome export 4 4.42E-7 0.49
6 SGTC_2026 5148700 4.86 152.0 µM 1.17E-6 2 5.95E-7 0.36
7 SGTC_679 0422-0091 4.53 41.4 µM 5.33E-6 Golgi 8 2.97E-6 0.08
8 SGTC_1264 0783-0040 4.53 158.0 µM 5.35E-6 2 2.98E-6 0.52
9 SGTC_931 2812-0076 4.42 566.0 µM 8.71E-6 2 4.99E-6 0.36
10 SGTC_67 0335-0847 4.18 266.0 µM 2.38E-5 ubiquinone biosynthesis & proteasome 18 1.44E-5 0.07
11 SGTC_2681 menadione 4.13 3.2 µM 2.94E-5 superoxide 3 1.80E-5 0.01
12 SGTC_35 0133-0054 4.13 64.1 µM 3.00E-5 5 1.84E-5 0.10
13 SGTC_3173 9105693 3.95 49.5 µM 6.06E-5 2 3.87E-5 0.14
14 SGTC_855 0428-0020 3.93 25.0 µM 6.55E-5 Golgi 7 4.20E-5 0.10
15 SGTC_561 1550-0330 3.90 72.9 µM 7.34E-5 4 4.74E-5 0.01
16 SGTC_97 0535-0701 3.89 19.1 µM 7.59E-5 60S ribosome export 10 4.91E-5 0.12
17 SGTC_2266 7929956 3.86 200.0 µM 8.63E-5 4 5.62E-5 0.16
18 SGTC_752 1896-0090 3.80 130.0 µM 1.08E-4 TSC3-RPN4 6 7.12E-5 0.06
19 SGTC_2829 7992743 3.78 58.4 µM 1.19E-4 8 7.90E-5 0.23
20 SGTC_1609 st001574 3.74 46.4 µM 1.36E-4 5 9.09E-5 0.17

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.204 8.94E-33 YPL244C HUT1 hom Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.200 1.66E-31 YDL045C FAD1 het Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.188 4.61E-28 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.160 1.42E-20 YMR161W HLJ1 hom Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.124 6.23E-13 YFL031W HAC1 hom Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
0.115 2.37E-11 YPL243W SRP68 het Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
0.114 3.81E-11 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.113 5.61E-11 YER087C-A_d YER087C-A_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase
0.103 2.27E-9 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.098 1.34E-8 YAL058W CNE1 hom Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.095 3.03E-8 YPL188W POS5 hom Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress
0.095 4.10E-8 YLR044C PDC1 hom Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism
0.093 8.09E-8 YDL209C CWC2 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
0.092 9.71E-8 YHL020C OPI1 hom Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance
0.091 1.41E-7 YCL051W LRE1 hom Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway;
LRE1 has a paralog, HLR1, that arose from the whole genome duplication