YCL069W / VBA3

Permease of basic amino acids in the vacuolar membrane

Zygosity: Homozygous strain
fixedexpanded
Profile for YCL069W / VBA3

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:3.72|P-value:9.24E-5 ID:SGTC_46|Compound:0329-0187a|FD-Score:4.79|P-value:7.26E-7 ID:SGTC_218|Compound:alverine citrate|FD-Score:3.20|P-value:6.61E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:4.79|P-value:7.33E-7 ID:SGTC_230|Compound:MPP+|FD-Score:-3.43|P-value:2.66E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:4.65|P-value:1.47E-6 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:-3.69|P-value:9.69E-5 ID:SGTC_356|Compound:1634-0012|FD-Score:3.95|P-value:3.57E-5 ID:SGTC_442|Compound:cape|FD-Score:5.43|P-value:2.44E-8 ID:SGTC_456|Compound:gingerol|FD-Score:5.94|P-value:1.14E-9 ID:SGTC_595|Compound:3909-8003|FD-Score:3.80|P-value:6.65E-5 ID:SGTC_713|Compound:1488-1073|FD-Score:3.24|P-value:5.68E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:5.21|P-value:8.02E-8 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.12|P-value:8.10E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:-3.16|P-value:7.25E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.70|P-value:9.88E-5 ID:SGTC_1251|Compound:0490-5699|FD-Score:-3.54|P-value:1.79E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.34|P-value:3.77E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.49|P-value:2.31E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.09|P-value:9.63E-4 ID:SGTC_1686|Compound:st019653|FD-Score:3.12|P-value:8.52E-4 ID:SGTC_1864|Compound:st057645|FD-Score:3.08|P-value:9.83E-4 ID:SGTC_1930|Compound:st074634|FD-Score:4.42|P-value:4.43E-6 ID:SGTC_1978|Compound:st071761|FD-Score:5.84|P-value:2.19E-9 ID:SGTC_2024|Compound:5144227|FD-Score:-3.58|P-value:1.53E-4 ID:SGTC_2086|Compound:5274770|FD-Score:4.51|P-value:2.95E-6 ID:SGTC_2094|Compound:5332512|FD-Score:3.36|P-value:3.69E-4 ID:SGTC_2321|Compound:7390090|FD-Score:3.41|P-value:3.08E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-4.02|P-value:2.50E-5 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.20|P-value:6.50E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.82|P-value:6.16E-5 ID:SGTC_2557|Compound:derrustone|FD-Score:5.98|P-value:9.09E-10 ID:SGTC_2956|Compound:9076196|FD-Score:3.39|P-value:3.34E-4 ID:SGTC_3132|Compound:9126706|FD-Score:-3.12|P-value:8.27E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:3.72|P-value:9.24E-5 ID:SGTC_46|Compound:0329-0187a|FD-Score:4.79|P-value:7.26E-7 ID:SGTC_218|Compound:alverine citrate|FD-Score:3.20|P-value:6.61E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:4.79|P-value:7.33E-7 ID:SGTC_230|Compound:MPP+|FD-Score:-3.43|P-value:2.66E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:4.65|P-value:1.47E-6 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:-3.69|P-value:9.69E-5 ID:SGTC_356|Compound:1634-0012|FD-Score:3.95|P-value:3.57E-5 ID:SGTC_442|Compound:cape|FD-Score:5.43|P-value:2.44E-8 ID:SGTC_456|Compound:gingerol|FD-Score:5.94|P-value:1.14E-9 ID:SGTC_595|Compound:3909-8003|FD-Score:3.80|P-value:6.65E-5 ID:SGTC_713|Compound:1488-1073|FD-Score:3.24|P-value:5.68E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:5.21|P-value:8.02E-8 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.12|P-value:8.10E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:-3.16|P-value:7.25E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.70|P-value:9.88E-5 ID:SGTC_1251|Compound:0490-5699|FD-Score:-3.54|P-value:1.79E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.34|P-value:3.77E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.49|P-value:2.31E-4 ID:SGTC_1673|Compound:st016611|FD-Score:3.09|P-value:9.63E-4 ID:SGTC_1686|Compound:st019653|FD-Score:3.12|P-value:8.52E-4 ID:SGTC_1864|Compound:st057645|FD-Score:3.08|P-value:9.83E-4 ID:SGTC_1930|Compound:st074634|FD-Score:4.42|P-value:4.43E-6 ID:SGTC_1978|Compound:st071761|FD-Score:5.84|P-value:2.19E-9 ID:SGTC_2024|Compound:5144227|FD-Score:-3.58|P-value:1.53E-4 ID:SGTC_2086|Compound:5274770|FD-Score:4.51|P-value:2.95E-6 ID:SGTC_2094|Compound:5332512|FD-Score:3.36|P-value:3.69E-4 ID:SGTC_2321|Compound:7390090|FD-Score:3.41|P-value:3.08E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-4.02|P-value:2.50E-5 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.20|P-value:6.50E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.82|P-value:6.16E-5 ID:SGTC_2557|Compound:derrustone|FD-Score:5.98|P-value:9.09E-10 ID:SGTC_2956|Compound:9076196|FD-Score:3.39|P-value:3.34E-4 ID:SGTC_3132|Compound:9126706|FD-Score:-3.12|P-value:8.27E-4

Top fitness defect scores for YCL069W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2557 derrustone 5.98 83.8 µM 9.09E-10 7 1.12E-9
2 SGTC_456 gingerol 5.94 170.0 µM 1.14E-9 4 1.40E-9
3 SGTC_1978 st071761 5.84 42.0 µM 2.19E-9 TSC3-RPN4 5 2.67E-9
4 SGTC_442 cape 5.43 100.0 µM 2.44E-8 1 2.88E-8
5 SGTC_1014 4130-3005 5.21 69.3 µM 8.02E-8 5 9.35E-8
6 SGTC_46 0329-0187a 4.79 2.2 µM 7.26E-7 45 8.25E-7
7 SGTC_226 1,3-diallylurea 4.79 8.3 mM 7.33E-7 6 8.33E-7
8 SGTC_231 b-estradiol 4.65 524.0 µM 1.47E-6 4 1.66E-6
9 SGTC_2086 5274770 4.51 176.6 µM 2.95E-6 12 3.30E-6
10 SGTC_1930 st074634 4.42 34.3 µM 4.43E-6 RNA pol III & RNase P/MRP 25 4.92E-6
11 SGTC_356 1634-0012 3.95 32.5 µM 3.57E-5 24 3.88E-5
12 SGTC_2541 muurolladie-3-one 3.82 83.1 µM 6.16E-5 excess fatty acid 32 6.65E-5
13 SGTC_595 3909-8003 3.80 73.7 µM 6.65E-5 12 7.17E-5
14 SGTC_37 0566-0070 3.72 1.6 µM 9.24E-5 37 9.93E-5
15 SGTC_1155 4088-0394 3.70 48.2 µM 9.88E-5 39 1.06E-4
16 SGTC_1479 0549-0158 3.49 134.0 µM 2.31E-4 48 2.45E-4
17 SGTC_2321 7390090 3.41 138.8 µM 3.08E-4 48 3.27E-4
18 SGTC_2956 9076196 3.39 2.5 µM 3.34E-4 39 3.54E-4
19 SGTC_2094 5332512 3.36 191.7 µM 3.69E-4 46 3.90E-4
20 SGTC_713 1488-1073 3.24 324.0 µM 5.68E-4 ERG2 29 5.98E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.137 1.81E-15 YKL182W FAS1 het Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
0.130 4.03E-14 YHR025W THR1 hom Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.115 2.77E-11 YDR530C APA2 hom Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
0.114 3.22E-11 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.109 2.13E-10 YGR161W-C_p YGR161W-C_p hom Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species
0.102 2.86E-9 YPR047W MSF1 hom Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase
0.102 2.98E-9 YMR222C FSH2 hom Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2
0.097 1.83E-8 YFR015C GSY1 hom Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.092 8.93E-8 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.087 3.93E-7 YNL301C RPL18B hom Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication
0.086 5.79E-7 YDL238C GUD1 hom Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures
0.086 6.71E-7 YGR044C RME1 hom Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.084 1.09E-6 YDR128W MTC5 hom Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress
0.083 1.49E-6 YGL138C_p YGL138C_p hom Putative protein of unknown function; has no significant sequence similarity to any known protein
0.082 1.76E-6 YGR092W DBF2 hom Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis