HIPHOP chemogenomics database

YCR073C / SSK22

MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p

Zygosity: Homozygous strain

fixedexpanded
Profile for YCR073C / SSK22

Click on Significant Values for Screen Details

Top fitness defect scores for YCR073C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2780	5215298	6.24	71.4 µM	5.81E-11		7	2.18E-10
2	SGTC_2999	9069765	4.36	71.4 µM	3.17E-6	amide catabolism	15	6.52E-6
3	SGTC_2324	6149169	4.18	200.0 µM	7.36E-6		17	1.44E-5
4	SGTC_1941	st076221	3.94	61.8 µM	2.17E-5	sphingolipid biosynthesis & PDR1	81	4.01E-5
5	SGTC_955	1216-0110	3.80	33.0 µM	4.14E-5	sphingolipid biosynthesis & PDR1	75	7.37E-5
6	SGTC_142	0335-0849	3.74	62.7 µM	5.34E-5	ubiquinone biosynthesis & proteasome	138	9.37E-5
7	SGTC_1380	2291-0730	3.51	164.0 µM	1.34E-4		38	2.23E-4
8	SGTC_1230	0170-0365	3.41	3.8 µM	2.03E-4	endomembrane recycling	98	3.30E-4
9	SGTC_3002	9075603	3.38	71.4 µM	2.23E-4		44	3.60E-4
10	SGTC_137	0438-0332	3.35	85.4 µM	2.56E-4	ubiquinone biosynthesis & proteasome	157	4.11E-4
11	SGTC_2929	9013348	3.29	24.6 µM	3.11E-4		50	4.94E-4
12	SGTC_376	3970-0862	3.13	59.1 µM	5.73E-4		52	8.78E-4
13	SGTC_367	0335-0881	3.09	7.1 µM	6.62E-4	ubiquinone biosynthesis & proteasome	130	0.00101
14	SGTC_139	0099-0334	3.06	718.6 µM	7.32E-4		79	0.00111
15	SGTC_1381	2324-0066	3.00	62.1 µM	9.10E-4		76	0.00136
16	SGTC_2823	7987624	2.86	58.4 µM	0.00144		103	0.00210
17	SGTC_837	0091-0265	2.86	12.5 µM	0.00148	ubiquinone biosynthesis & proteasome	70	0.00215
18	SGTC_2504	purpurogallin	2.78	52.3 µM	0.00191	RPP1 & pyrimidine depletion	75	0.00273
19	SGTC_2921	7974240	2.77	10.7 µM	0.00198		118	0.00283
20	SGTC_763	3453-1109	2.76	128.0 µM	0.00200		74	0.00285

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.158	4.81E-20	YDR274C_d	YDR274C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.148	8.08E-18	YJL193W_p	YJL193W_p	hom	Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.143	1.01E-16	YDR326C	YSP2	hom	Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
0.124	5.59E-13	YPR065W	ROX1	hom	Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance
0.123	9.43E-13	YER012W	PRE1	het	Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.120	3.55E-12	YDL183C	YDL183C	hom	Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
0.119	4.43E-12	YML034C-A_d	YML034C-A_d	hom	Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.119	4.86E-12	YOR165W	SEY1	hom	Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3
0.117	1.03E-11	YBR165W	UBS1	hom	Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity
0.117	1.19E-11	YDL020C	RPN4	hom	Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
0.115	2.73E-11	YJL155C	FBP26	hom	Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
0.113	4.48E-11	YCR089W	FIG2	hom	Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating
0.111	1.28E-10	YER067W	RGI1	hom	Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress
0.109	2.34E-10	YGL194C	HOS2	hom	Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS
0.108	3.44E-10	YOR254C	SEC63	het	Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER