HIPHOP chemogenomics database

YDL007W / RPT2

One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle

Zygosity: Heterozygous strain

fixedexpanded
Profile for YDL007W / RPT2

Click on Significant Values for Screen Details

Top fitness defect scores for YDL007W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_3142	9086498	6.26	49.5 µM	4.02E-11	iron homeostasis	23	1.87E-10	0.31
2	SGTC_2575	plumbagin	5.84	510.0 nM	6.77E-10	superoxide	13	2.63E-9	0.41
3	SGTC_2344	7990960	5.84	77.0 µM	6.83E-10		19	2.65E-9	0.11
4	SGTC_2124	5246320	5.60	200.0 µM	3.07E-9	heme biosynthesis & mitochondrial translocase	18	1.08E-8	0.12
5	SGTC_490	phenamil	5.59	164.0 µM	3.24E-9	heme requiring	2	1.14E-8	0.61
6	SGTC_462	ag-879	5.13	158.0 µM	5.00E-8		5	1.47E-7	0.15
7	SGTC_2019	4016004	4.79	108.0 µM	3.12E-7		6	8.15E-7	0.12
8	SGTC_339	3091-4833	4.39	71.4 µM	2.45E-6	iron homeostasis	7	5.59E-6	0.23
9	SGTC_2962	9082311	4.08	13.2 µM	1.11E-5		10	2.30E-5	0.21
10	SGTC_2014	4013930	3.98	200.0 µM	1.73E-5		3	3.47E-5	0.11
11	SGTC_1309	pyrazolanthrone	3.96	13.4 µM	1.90E-5		9	3.79E-5	0.15
12	SGTC_431	k089-0097	3.90	107.0 µM	2.43E-5	iron homeostasis	16	4.78E-5	0.08
13	SGTC_410	α-linolenic acid	3.64	100.0 µM	7.46E-5	excess fatty acid	8	1.36E-4	0.05
14	SGTC_1725	st036284	3.54	20.6 µM	1.12E-4		7	2.00E-4	0.09
15	SGTC_186	3455-3465	3.50	67.3 µM	1.33E-4		4	2.34E-4	0.15
16	SGTC_444	curcumin	3.38	90.4 µM	2.16E-4	iron homeostasis	24	3.67E-4	0.04
17	SGTC_2159	5658611	3.34	73.8 µM	2.45E-4		11	4.14E-4	0.08
18	SGTC_3126	9127922	3.29	49.5 µM	2.99E-4	fatty acid desaturase (OLE1)	43	4.98E-4	0.04
19	SGTC_474	dl-dihydrosphingosine	3.20	5.0 µM	4.17E-4		16	6.81E-4	0.07
20	SGTC_3080	9117754	3.19	49.5 µM	4.35E-4		11	7.08E-4	3.95E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.277	4.45E-60	YOR117W	RPT5	het	One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
0.271	1.14E-57	YOR159C	SME1	het	Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.246	1.30E-47	YGL048C	RPT6	het	ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.242	9.11E-46	YDR427W	RPN9	het	Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
0.237	5.13E-44	YDL060W	TSR1	het	Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.233	1.31E-42	YBL041W	PRE7	het	Beta 6 subunit of the 20S proteasome
0.233	1.18E-42	YHR166C	CDC23	het	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.228	1.01E-40	YFR004W	RPN11	het	Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
0.216	7.45E-37	YPR103W	PRE2	het	Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
0.201	5.47E-32	YOR259C	RPT4	het	One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
0.196	2.04E-30	YNL161W	CBK1	het	Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
0.191	7.78E-29	YFR050C	PRE4	het	Beta 7 subunit of the 20S proteasome
0.180	8.28E-26	YIL083C	CAB2	het	Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
0.172	8.05E-24	YAR008W	SEN34	het	Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
0.170	3.55E-23	YOR304W	ISW2	hom	ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth