YDL019C / OSH2

Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL019C / OSH2

Click on Significant Values for Screen Details ID:SGTC_47|Compound:0149-0135|FD-Score:4.38|P-value:3.97E-5 ID:SGTC_54|Compound:0159-0055|FD-Score:4.15|P-value:9.18E-5 ID:SGTC_146|Compound:3448-8650|FD-Score:3.85|P-value:2.64E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.47|P-value:8.98E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.73|P-value:3.32E-4 ID:SGTC_489|Compound:paxilline|FD-Score:-3.79|P-value:2.72E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:9.41|P-value:9.25E-18 ID:SGTC_520|Compound:3584-0023|FD-Score:5.24|P-value:1.17E-6 ID:SGTC_528|Compound:3492-0019|FD-Score:8.50|P-value:8.06E-15 ID:SGTC_631|Compound:k072-0230|FD-Score:-4.82|P-value:5.64E-6 ID:SGTC_999|Compound:4022-0066|FD-Score:3.62|P-value:5.73E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:-3.63|P-value:4.57E-4 ID:SGTC_1439|Compound:4092-1168|FD-Score:3.58|P-value:6.40E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:4.34|P-value:4.68E-5 ID:SGTC_1563|Compound:conessine|FD-Score:4.49|P-value:2.60E-5 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:4.35|P-value:4.47E-5 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.51|P-value:8.00E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.81|P-value:3.08E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.78|P-value:3.36E-4 ID:SGTC_1712|Compound:st032288|FD-Score:11.50|P-value:1.63E-25 ID:SGTC_1738|Compound:st035555|FD-Score:-3.58|P-value:5.39E-4 ID:SGTC_1810|Compound:st050138|FD-Score:3.92|P-value:2.09E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.96|P-value:1.84E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.35|P-value:7.17E-7 ID:SGTC_2027|Compound:5150920|FD-Score:-4.27|P-value:4.98E-5 ID:SGTC_2062|Compound:5245942|FD-Score:3.64|P-value:5.26E-4 ID:SGTC_2078|Compound:5212016|FD-Score:3.89|P-value:2.28E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-3.59|P-value:5.23E-4 ID:SGTC_2338|Compound:7999974|FD-Score:3.80|P-value:3.12E-4 ID:SGTC_2516|Compound:gossypol|FD-Score:3.59|P-value:6.11E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.77|P-value:2.91E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.24|P-value:4.96E-14 ID:SGTC_2773|Compound:thioridazine|FD-Score:-3.43|P-value:8.54E-4 ID:SGTC_2896|Compound:9052656|FD-Score:4.20|P-value:7.71E-5 ID:SGTC_3337|Compound:9144342|FD-Score:3.94|P-value:1.98E-4 ID:SGTC_3345|Compound:9148410|FD-Score:3.69|P-value:4.45E-4 ID:SGTC_47|Compound:0149-0135|FD-Score:4.38|P-value:3.97E-5 ID:SGTC_54|Compound:0159-0055|FD-Score:4.15|P-value:9.18E-5 ID:SGTC_146|Compound:3448-8650|FD-Score:3.85|P-value:2.64E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.47|P-value:8.98E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.73|P-value:3.32E-4 ID:SGTC_489|Compound:paxilline|FD-Score:-3.79|P-value:2.72E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:9.41|P-value:9.25E-18 ID:SGTC_520|Compound:3584-0023|FD-Score:5.24|P-value:1.17E-6 ID:SGTC_528|Compound:3492-0019|FD-Score:8.50|P-value:8.06E-15 ID:SGTC_631|Compound:k072-0230|FD-Score:-4.82|P-value:5.64E-6 ID:SGTC_999|Compound:4022-0066|FD-Score:3.62|P-value:5.73E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:-3.63|P-value:4.57E-4 ID:SGTC_1439|Compound:4092-1168|FD-Score:3.58|P-value:6.40E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:4.34|P-value:4.68E-5 ID:SGTC_1563|Compound:conessine|FD-Score:4.49|P-value:2.60E-5 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:4.35|P-value:4.47E-5 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.51|P-value:8.00E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.81|P-value:3.08E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.78|P-value:3.36E-4 ID:SGTC_1712|Compound:st032288|FD-Score:11.50|P-value:1.63E-25 ID:SGTC_1738|Compound:st035555|FD-Score:-3.58|P-value:5.39E-4 ID:SGTC_1810|Compound:st050138|FD-Score:3.92|P-value:2.09E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.96|P-value:1.84E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.35|P-value:7.17E-7 ID:SGTC_2027|Compound:5150920|FD-Score:-4.27|P-value:4.98E-5 ID:SGTC_2062|Compound:5245942|FD-Score:3.64|P-value:5.26E-4 ID:SGTC_2078|Compound:5212016|FD-Score:3.89|P-value:2.28E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-3.59|P-value:5.23E-4 ID:SGTC_2338|Compound:7999974|FD-Score:3.80|P-value:3.12E-4 ID:SGTC_2516|Compound:gossypol|FD-Score:3.59|P-value:6.11E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.77|P-value:2.91E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.24|P-value:4.96E-14 ID:SGTC_2773|Compound:thioridazine|FD-Score:-3.43|P-value:8.54E-4 ID:SGTC_2896|Compound:9052656|FD-Score:4.20|P-value:7.71E-5 ID:SGTC_3337|Compound:9144342|FD-Score:3.94|P-value:1.98E-4 ID:SGTC_3345|Compound:9148410|FD-Score:3.69|P-value:4.45E-4

Top fitness defect scores for YDL019C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1712 st032288 11.50 53.4 µM 1.63E-25 calcium & mitochondrial duress 13 8.93E-31
2 SGTC_515 4092-1064 9.41 102.0 µM 9.25E-18 plasma membrane duress 9 2.50E-21
3 SGTC_528 3492-0019 8.50 172.0 µM 8.06E-15 calcium & mitochondrial duress 61 9.54E-18
4 SGTC_2688 kanamycin b 8.24 96.7 µM 4.96E-14 calcium & mitochondrial duress 58 8.72E-17
5 SGTC_2021 4019055 5.35 173.0 µM 7.17E-7 endomembrane recycling 58 4.51E-8
6 SGTC_520 3584-0023 5.24 682.0 µM 1.17E-6 9 8.21E-8
7 SGTC_1563 conessine 4.49 56.1 µM 2.60E-5 8 3.54E-6
8 SGTC_47 0149-0135 4.38 24.2 µM 3.97E-5 TSC3-RPN4 15 5.89E-6
9 SGTC_1566 6,7-dimethoxy-4-ethylcoumarin 4.35 85.4 µM 4.47E-5 15 6.81E-6
10 SGTC_1512 0259-0544 4.34 12.2 µM 4.68E-5 8 7.20E-6
11 SGTC_2896 9052656 4.20 71.4 µM 7.71E-5 endomembrane recycling 95 1.32E-5
12 SGTC_54 0159-0055 4.15 9.0 µM 9.18E-5 ERG2 42 1.63E-5
13 SGTC_1951 sophoraflavanone G 3.96 9.2 µM 1.84E-4 RNA processing & uracil transport 21 3.79E-5
14 SGTC_3337 9144342 3.94 33.5 µM 1.98E-4 27 4.13E-5
15 SGTC_1810 st050138 3.92 53.0 µM 2.09E-4 PDR1 11 4.42E-5
16 SGTC_2078 5212016 3.89 200.0 µM 2.28E-4 60S ribosome export 16 4.92E-5
17 SGTC_146 3448-8650 3.85 53.1 µM 2.64E-4 NEO1-PIK1 144 5.87E-5
18 SGTC_1572 epiandrosterone 3.81 68.9 µM 3.08E-4 32 7.07E-5
19 SGTC_2338 7999974 3.80 200.0 µM 3.12E-4 28 7.19E-5
20 SGTC_1597 7-benzyloxygramine 3.78 47.5 µM 3.36E-4 calcium & mitochondrial duress 133 7.85E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.395 7.48E-126 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.377 7.20E-114 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.359 1.41E-102 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.324 1.08E-82 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.322 9.11E-82 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.293 1.19E-67 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.280 2.70E-61 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.273 3.41E-58 YGR237C_p YGR237C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.270 5.20E-57 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.266 1.66E-55 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.265 6.93E-55 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.255 5.72E-51 YGR170W PSD2 hom Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine
0.253 4.26E-50 YPL145C KES1 hom One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
0.251 2.69E-49 YML062C MFT1 hom Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.249 1.79E-48 YBL022C PIM1 hom ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria