YDL033C / SLM3

tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF)

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL033C / SLM3

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-3.69|P-value:4.07E-4 ID:SGTC_85|Compound:0868-0259|FD-Score:-3.62|P-value:5.14E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:4.42|P-value:2.77E-5 ID:SGTC_126|Compound:0417-1624|FD-Score:4.80|P-value:5.80E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:4.75|P-value:7.26E-6 ID:SGTC_157|Compound:1348-1695|FD-Score:3.82|P-value:2.39E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:10.00|P-value:2.93E-20 ID:SGTC_301|Compound:4119-0100|FD-Score:5.21|P-value:9.81E-7 ID:SGTC_311|Compound:1315-0136|FD-Score:3.64|P-value:4.45E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:4.10|P-value:9.10E-5 ID:SGTC_364|Compound:0083-0097|FD-Score:3.73|P-value:3.33E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:3.49|P-value:7.21E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.58|P-value:5.89E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.71|P-value:9.54E-6 ID:SGTC_702|Compound:1488-0190|FD-Score:-4.18|P-value:7.39E-5 ID:SGTC_720|Compound:r052-1644|FD-Score:3.39|P-value:9.84E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:4.41|P-value:2.82E-5 ID:SGTC_1017|Compound:4239-0030|FD-Score:3.81|P-value:2.51E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.58|P-value:5.43E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:-3.52|P-value:7.13E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:7.99|P-value:1.62E-13 ID:SGTC_1189|Compound:1431-2119|FD-Score:4.79|P-value:6.11E-6 ID:SGTC_1534|Compound:6-azauridine|FD-Score:5.06|P-value:1.96E-6 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.48|P-value:7.32E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.64|P-value:4.47E-4 ID:SGTC_1735|Compound:st034310|FD-Score:3.66|P-value:4.18E-4 ID:SGTC_1745|Compound:st081495|FD-Score:4.63|P-value:1.19E-5 ID:SGTC_1754|Compound:st042065|FD-Score:3.83|P-value:2.34E-4 ID:SGTC_1760|Compound:st045453|FD-Score:4.69|P-value:9.25E-6 ID:SGTC_1781|Compound:st038725|FD-Score:8.57|P-value:2.82E-15 ID:SGTC_1800|Compound:st050079|FD-Score:3.61|P-value:4.89E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.50|P-value:6.85E-4 ID:SGTC_1803|Compound:st050068|FD-Score:5.45|P-value:3.26E-7 ID:SGTC_1850|Compound:st056238|FD-Score:4.38|P-value:3.18E-5 ID:SGTC_2023|Compound:5144124|FD-Score:4.94|P-value:3.25E-6 ID:SGTC_2026|Compound:5148700|FD-Score:4.26|P-value:4.98E-5 ID:SGTC_2028|Compound:5153890|FD-Score:4.43|P-value:2.58E-5 ID:SGTC_2142|Compound:5622915|FD-Score:3.56|P-value:5.62E-4 ID:SGTC_2189|Compound:6047310|FD-Score:3.51|P-value:6.65E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.68|P-value:3.89E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.54|P-value:6.62E-4 ID:SGTC_2694|Compound:st077685|FD-Score:3.49|P-value:7.10E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.69|P-value:3.76E-4 ID:SGTC_2815|Compound:7968870|FD-Score:-4.13|P-value:9.18E-5 ID:SGTC_2892|Compound:9047358|FD-Score:3.92|P-value:1.74E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.26|P-value:5.66E-5 ID:SGTC_2943|Compound:9068529|FD-Score:3.84|P-value:2.24E-4 ID:SGTC_2966|Compound:9084041|FD-Score:4.44|P-value:2.51E-5 ID:SGTC_3037|Compound:9091026|FD-Score:5.17|P-value:1.19E-6 ID:SGTC_3123|Compound:9126162|FD-Score:3.60|P-value:5.10E-4 ID:SGTC_3182|Compound:9108849|FD-Score:3.53|P-value:6.20E-4 ID:SGTC_3324|Compound:9138759|FD-Score:-4.01|P-value:1.40E-4 ID:SGTC_3331|Compound:9141060|FD-Score:3.81|P-value:2.51E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.69|P-value:4.07E-4 ID:SGTC_85|Compound:0868-0259|FD-Score:-3.62|P-value:5.14E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:4.42|P-value:2.77E-5 ID:SGTC_126|Compound:0417-1624|FD-Score:4.80|P-value:5.80E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:4.75|P-value:7.26E-6 ID:SGTC_157|Compound:1348-1695|FD-Score:3.82|P-value:2.39E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:10.00|P-value:2.93E-20 ID:SGTC_301|Compound:4119-0100|FD-Score:5.21|P-value:9.81E-7 ID:SGTC_311|Compound:1315-0136|FD-Score:3.64|P-value:4.45E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:4.10|P-value:9.10E-5 ID:SGTC_364|Compound:0083-0097|FD-Score:3.73|P-value:3.33E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:3.49|P-value:7.21E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.58|P-value:5.89E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.71|P-value:9.54E-6 ID:SGTC_702|Compound:1488-0190|FD-Score:-4.18|P-value:7.39E-5 ID:SGTC_720|Compound:r052-1644|FD-Score:3.39|P-value:9.84E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:4.41|P-value:2.82E-5 ID:SGTC_1017|Compound:4239-0030|FD-Score:3.81|P-value:2.51E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.58|P-value:5.43E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:-3.52|P-value:7.13E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:7.99|P-value:1.62E-13 ID:SGTC_1189|Compound:1431-2119|FD-Score:4.79|P-value:6.11E-6 ID:SGTC_1534|Compound:6-azauridine|FD-Score:5.06|P-value:1.96E-6 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.48|P-value:7.32E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.64|P-value:4.47E-4 ID:SGTC_1735|Compound:st034310|FD-Score:3.66|P-value:4.18E-4 ID:SGTC_1745|Compound:st081495|FD-Score:4.63|P-value:1.19E-5 ID:SGTC_1754|Compound:st042065|FD-Score:3.83|P-value:2.34E-4 ID:SGTC_1760|Compound:st045453|FD-Score:4.69|P-value:9.25E-6 ID:SGTC_1781|Compound:st038725|FD-Score:8.57|P-value:2.82E-15 ID:SGTC_1800|Compound:st050079|FD-Score:3.61|P-value:4.89E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.50|P-value:6.85E-4 ID:SGTC_1803|Compound:st050068|FD-Score:5.45|P-value:3.26E-7 ID:SGTC_1850|Compound:st056238|FD-Score:4.38|P-value:3.18E-5 ID:SGTC_2023|Compound:5144124|FD-Score:4.94|P-value:3.25E-6 ID:SGTC_2026|Compound:5148700|FD-Score:4.26|P-value:4.98E-5 ID:SGTC_2028|Compound:5153890|FD-Score:4.43|P-value:2.58E-5 ID:SGTC_2142|Compound:5622915|FD-Score:3.56|P-value:5.62E-4 ID:SGTC_2189|Compound:6047310|FD-Score:3.51|P-value:6.65E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.68|P-value:3.89E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.54|P-value:6.62E-4 ID:SGTC_2694|Compound:st077685|FD-Score:3.49|P-value:7.10E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.69|P-value:3.76E-4 ID:SGTC_2815|Compound:7968870|FD-Score:-4.13|P-value:9.18E-5 ID:SGTC_2892|Compound:9047358|FD-Score:3.92|P-value:1.74E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.26|P-value:5.66E-5 ID:SGTC_2943|Compound:9068529|FD-Score:3.84|P-value:2.24E-4 ID:SGTC_2966|Compound:9084041|FD-Score:4.44|P-value:2.51E-5 ID:SGTC_3037|Compound:9091026|FD-Score:5.17|P-value:1.19E-6 ID:SGTC_3123|Compound:9126162|FD-Score:3.60|P-value:5.10E-4 ID:SGTC_3182|Compound:9108849|FD-Score:3.53|P-value:6.20E-4 ID:SGTC_3324|Compound:9138759|FD-Score:-4.01|P-value:1.40E-4 ID:SGTC_3331|Compound:9141060|FD-Score:3.81|P-value:2.51E-4

Top fitness defect scores for YDL033C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_221 trifluoperazine 10.00 10.3 µM 2.93E-20 NEO1-PIK1 58 5.24E-24
2 SGTC_1781 st038725 8.57 66.3 µM 2.82E-15 NEO1 27 5.18E-18
3 SGTC_1130 3454-2858 7.99 15.9 µM 1.62E-13 NEO1 23 6.78E-16
4 SGTC_1803 st050068 5.45 52.4 µM 3.26E-7 NEO1-PIK1 97 2.48E-8
5 SGTC_301 4119-0100 5.21 24.5 µM 9.81E-7 NEO1 48 9.28E-8
6 SGTC_3037 9091026 5.17 49.5 µM 1.19E-6 NEO1-PIK1 71 1.17E-7
7 SGTC_1534 6-azauridine 5.06 81.6 µM 1.96E-6 RNA pol III & RNase P/MRP 9 2.13E-7
8 SGTC_2023 5144124 4.94 124.0 µM 3.25E-6 10 3.90E-7
9 SGTC_126 0417-1624 4.80 672.5 µM 5.80E-6 DNA intercalators 11 7.78E-7
10 SGTC_1189 1431-2119 4.79 7.3 µM 6.11E-6 10 8.28E-7
11 SGTC_146 3448-8650 4.75 53.1 µM 7.26E-6 NEO1-PIK1 98 1.02E-6
12 SGTC_1760 st045453 4.69 10.4 µM 9.25E-6 NEO1-PIK1 118 1.36E-6
13 SGTC_1745 st081495 4.63 74.0 µM 1.19E-5 RNA processing & uracil transport 53 1.84E-6
14 SGTC_2966 9084041 4.44 48.0 µM 2.51E-5 23 4.47E-6
15 SGTC_2028 5153890 4.43 36.4 µM 2.58E-5 cell wall signaling 26 4.63E-6
16 SGTC_98 0074-0023 4.42 107.7 µM 2.77E-5 Golgi 28 5.04E-6
17 SGTC_752 1896-0090 4.41 130.0 µM 2.82E-5 TSC3-RPN4 10 5.15E-6
18 SGTC_1850 st056238 4.38 17.0 µM 3.18E-5 RPP1 & pyrimidine depletion 13 5.94E-6
19 SGTC_2026 5148700 4.26 152.0 µM 4.98E-5 13 1.01E-5
20 SGTC_313 3474-0040 4.10 5.0 µM 9.10E-5 NEO1-PIK1 68 2.08E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.365 5.97E-106 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.310 1.47E-75 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.310 1.54E-75 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.304 1.28E-72 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.301 6.46E-71 YOR293W RPS10A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.301 6.94E-71 YOR039W CKB2 hom Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.292 9.06E-67 YIL048W NEO1 het Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
0.284 5.22E-63 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.282 2.72E-62 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.281 1.04E-61 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.266 1.55E-55 YGL072C_d YGL072C_d hom Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
0.249 1.86E-48 YAL009W SPO7 hom Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation
0.247 8.89E-48 YJL124C LSM1 hom Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
0.246 2.13E-47 YML094C-A_d YML094C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
0.241 2.88E-45 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33