YDL104C / QRI7

Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL104C / QRI7

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:3.13|P-value:6.98E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.70|P-value:5.42E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.88|P-value:2.50E-5 ID:SGTC_192|Compound:3455-0609|FD-Score:-3.08|P-value:6.25E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.34|P-value:3.26E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.04|P-value:7.20E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:3.26|P-value:4.33E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:3.21|P-value:5.29E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.59|P-value:8.65E-5 ID:SGTC_773|Compound:4358-1457|FD-Score:4.58|P-value:1.33E-6 ID:SGTC_966|Compound:1300-0360|FD-Score:3.36|P-value:3.04E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.98|P-value:2.33E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.84|P-value:4.30E-5 ID:SGTC_1256|Compound:0570-0116|FD-Score:-3.80|P-value:3.50E-5 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.33|P-value:3.41E-4 ID:SGTC_1352|Compound:1496-0991|FD-Score:3.30|P-value:3.81E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.40|P-value:1.87E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.70|P-value:7.61E-5 ID:SGTC_1627|Compound:st003713|FD-Score:4.17|P-value:9.95E-6 ID:SGTC_1630|Compound:st005209|FD-Score:4.17|P-value:9.84E-6 ID:SGTC_1674|Compound:st016616|FD-Score:3.24|P-value:4.65E-4 ID:SGTC_1830|Compound:st055982|FD-Score:3.56|P-value:1.37E-4 ID:SGTC_1838|Compound:st055369|FD-Score:3.71|P-value:7.40E-5 ID:SGTC_1840|Compound:st055363|FD-Score:3.08|P-value:8.33E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-3.01|P-value:7.85E-4 ID:SGTC_2065|Compound:5221596|FD-Score:-3.17|P-value:4.52E-4 ID:SGTC_2347|Compound:9049783|FD-Score:-2.97|P-value:9.19E-4 ID:SGTC_2352|Compound:9027995|FD-Score:-6.84|P-value:5.57E-13 ID:SGTC_2365|Compound:9042475|FD-Score:3.28|P-value:4.02E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.04|P-value:7.06E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.36|P-value:3.05E-4 ID:SGTC_2411|Compound:st077133|FD-Score:4.11|P-value:1.29E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.43|P-value:2.25E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.42|P-value:2.34E-4 ID:SGTC_2694|Compound:st077685|FD-Score:-3.84|P-value:2.96E-5 ID:SGTC_2789|Compound:5281112|FD-Score:-3.05|P-value:6.89E-4 ID:SGTC_2794|Compound:7785382|FD-Score:4.27|P-value:6.15E-6 ID:SGTC_2900|Compound:5328528|FD-Score:-3.07|P-value:6.47E-4 ID:SGTC_2908|Compound:7930301|FD-Score:-3.91|P-value:2.20E-5 ID:SGTC_2914|Compound:7968218|FD-Score:-3.27|P-value:3.08E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-3.03|P-value:7.46E-4 ID:SGTC_3010|Compound:9080089|FD-Score:-3.30|P-value:2.72E-4 ID:SGTC_3029|Compound:9088683|FD-Score:5.23|P-value:4.02E-8 ID:SGTC_3137|Compound:9097352|FD-Score:4.03|P-value:1.87E-5 ID:SGTC_3138|Compound:9097216|FD-Score:-4.08|P-value:9.97E-6 ID:SGTC_3145|Compound:9085516|FD-Score:-4.05|P-value:1.15E-5 ID:SGTC_3326|Compound:9140064|FD-Score:3.05|P-value:9.30E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:3.13|P-value:6.98E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.70|P-value:5.42E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.88|P-value:2.50E-5 ID:SGTC_192|Compound:3455-0609|FD-Score:-3.08|P-value:6.25E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:3.34|P-value:3.26E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.04|P-value:7.20E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:3.26|P-value:4.33E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:3.21|P-value:5.29E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.59|P-value:8.65E-5 ID:SGTC_773|Compound:4358-1457|FD-Score:4.58|P-value:1.33E-6 ID:SGTC_966|Compound:1300-0360|FD-Score:3.36|P-value:3.04E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:3.98|P-value:2.33E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.84|P-value:4.30E-5 ID:SGTC_1256|Compound:0570-0116|FD-Score:-3.80|P-value:3.50E-5 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.33|P-value:3.41E-4 ID:SGTC_1352|Compound:1496-0991|FD-Score:3.30|P-value:3.81E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.40|P-value:1.87E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.70|P-value:7.61E-5 ID:SGTC_1627|Compound:st003713|FD-Score:4.17|P-value:9.95E-6 ID:SGTC_1630|Compound:st005209|FD-Score:4.17|P-value:9.84E-6 ID:SGTC_1674|Compound:st016616|FD-Score:3.24|P-value:4.65E-4 ID:SGTC_1830|Compound:st055982|FD-Score:3.56|P-value:1.37E-4 ID:SGTC_1838|Compound:st055369|FD-Score:3.71|P-value:7.40E-5 ID:SGTC_1840|Compound:st055363|FD-Score:3.08|P-value:8.33E-4 ID:SGTC_2029|Compound:5175081|FD-Score:-3.01|P-value:7.85E-4 ID:SGTC_2065|Compound:5221596|FD-Score:-3.17|P-value:4.52E-4 ID:SGTC_2347|Compound:9049783|FD-Score:-2.97|P-value:9.19E-4 ID:SGTC_2352|Compound:9027995|FD-Score:-6.84|P-value:5.57E-13 ID:SGTC_2365|Compound:9042475|FD-Score:3.28|P-value:4.02E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.04|P-value:7.06E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.36|P-value:3.05E-4 ID:SGTC_2411|Compound:st077133|FD-Score:4.11|P-value:1.29E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.43|P-value:2.25E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.42|P-value:2.34E-4 ID:SGTC_2694|Compound:st077685|FD-Score:-3.84|P-value:2.96E-5 ID:SGTC_2789|Compound:5281112|FD-Score:-3.05|P-value:6.89E-4 ID:SGTC_2794|Compound:7785382|FD-Score:4.27|P-value:6.15E-6 ID:SGTC_2900|Compound:5328528|FD-Score:-3.07|P-value:6.47E-4 ID:SGTC_2908|Compound:7930301|FD-Score:-3.91|P-value:2.20E-5 ID:SGTC_2914|Compound:7968218|FD-Score:-3.27|P-value:3.08E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-3.03|P-value:7.46E-4 ID:SGTC_3010|Compound:9080089|FD-Score:-3.30|P-value:2.72E-4 ID:SGTC_3029|Compound:9088683|FD-Score:5.23|P-value:4.02E-8 ID:SGTC_3137|Compound:9097352|FD-Score:4.03|P-value:1.87E-5 ID:SGTC_3138|Compound:9097216|FD-Score:-4.08|P-value:9.97E-6 ID:SGTC_3145|Compound:9085516|FD-Score:-4.05|P-value:1.15E-5 ID:SGTC_3326|Compound:9140064|FD-Score:3.05|P-value:9.30E-4

Top fitness defect scores for YDL104C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3029 9088683 5.23 49.5 µM 4.02E-8 9 8.48E-8
2 SGTC_773 4358-1457 4.58 44.6 µM 1.33E-6 27 2.30E-6
3 SGTC_2794 7785382 4.27 27.3 µM 6.15E-6 26 9.80E-6
4 SGTC_1630 st005209 4.17 31.3 µM 9.84E-6 20 1.53E-5
5 SGTC_1627 st003713 4.17 25.6 µM 9.95E-6 calcium & mitochondrial duress 64 1.55E-5
6 SGTC_2411 st077133 4.11 39.9 µM 1.29E-5 RNA pol III & RNase P/MRP 27 1.98E-5
7 SGTC_3137 9097352 4.03 49.5 µM 1.87E-5 39 2.81E-5
8 SGTC_1129 3453-1384 3.98 223.0 µM 2.33E-5 39 3.46E-5
9 SGTC_1137 ixabepilone 3.84 100.0 µM 4.30E-5 28 6.18E-5
10 SGTC_1838 st055369 3.71 84.5 µM 7.40E-5 25 1.03E-4
11 SGTC_1406 3909-7906 3.70 11.9 µM 7.61E-5 60S ribosome export 13 1.06E-4
12 SGTC_1830 st055982 3.56 50.3 µM 1.37E-4 32 1.85E-4
13 SGTC_2519 cryptotanshinone 3.43 88.0 µM 2.25E-4 heme biosynthesis & mitochondrial translocase 26 2.97E-4
14 SGTC_2687 chenodiol 3.42 100.0 µM 2.34E-4 40 3.08E-4
15 SGTC_966 1300-0360 3.36 30.2 µM 3.04E-4 heme requiring 47 3.95E-4
16 SGTC_2405 ticlatone 3.36 3.5 µM 3.05E-4 97 3.96E-4
17 SGTC_236 1509-0011 3.34 5.3 µM 3.26E-4 434 4.21E-4
18 SGTC_1288 0973-0013 3.33 65.5 µM 3.41E-4 38 4.40E-4
19 SGTC_1352 1496-0991 3.30 99.0 µM 3.81E-4 tubulin folding & SWR complex 48 4.88E-4
20 SGTC_2365 9042475 3.28 200.0 µM 4.02E-4 74 5.15E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.142 1.33E-16 YAL043C PTA1 het Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII
0.135 4.71E-15 YKL109W HAP4 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex
0.123 8.57E-13 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.118 8.24E-12 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.111 1.35E-10 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.108 3.14E-10 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.107 4.45E-10 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.106 8.79E-10 YGR112W SHY1 hom Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome
0.105 1.01E-9 YOR238W_p YOR238W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.105 1.13E-9 YOR010C TIR2 hom Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis
0.104 1.84E-9 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.103 2.52E-9 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.102 3.39E-9 YHL038C CBP2 hom Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene
0.102 3.68E-9 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.101 4.28E-9 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication