YDL105W / NSE4

Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL105W / NSE4

Click on Significant Values for Screen Details ID:SGTC_5|Compound:0352-0601|FD-Score:3.77|P-value:1.50E-4 ID:SGTC_13|Compound:0199-0087|FD-Score:3.50|P-value:3.93E-4 ID:SGTC_60|Compound:0986-0278|FD-Score:3.76|P-value:1.54E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:3.56|P-value:3.16E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.60|P-value:2.75E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.43|P-value:5.01E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:-5.67|P-value:1.61E-8 ID:SGTC_176|Compound:1486-1328|FD-Score:-4.34|P-value:1.12E-5 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.39|P-value:5.79E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:4.35|P-value:1.46E-5 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.62|P-value:2.58E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:3.43|P-value:5.07E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:6.79|P-value:2.92E-11 ID:SGTC_808|Compound:0711-0032|FD-Score:-3.44|P-value:3.71E-4 ID:SGTC_866|Compound:0495-0101|FD-Score:3.29|P-value:7.94E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:-3.69|P-value:1.50E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:-3.72|P-value:1.37E-4 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.86|P-value:1.07E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.23|P-value:9.76E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:4.43|P-value:1.04E-5 ID:SGTC_1834|Compound:st055981|FD-Score:-3.76|P-value:1.16E-4 ID:SGTC_1839|Compound:st053978|FD-Score:5.50|P-value:5.93E-8 ID:SGTC_1884|Compound:5633444|FD-Score:4.05|P-value:4.90E-5 ID:SGTC_1978|Compound:st071761|FD-Score:4.03|P-value:5.33E-5 ID:SGTC_2059|Compound:5238311|FD-Score:6.92|P-value:1.23E-11 ID:SGTC_2472|Compound:5377652|FD-Score:3.62|P-value:2.53E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.55|P-value:3.34E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-3.44|P-value:3.77E-4 ID:SGTC_3082|Compound:9117008|FD-Score:4.14|P-value:3.52E-5 ID:SGTC_5|Compound:0352-0601|FD-Score:3.77|P-value:1.50E-4 ID:SGTC_13|Compound:0199-0087|FD-Score:3.50|P-value:3.93E-4 ID:SGTC_60|Compound:0986-0278|FD-Score:3.76|P-value:1.54E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:3.56|P-value:3.16E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:3.60|P-value:2.75E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.43|P-value:5.01E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:-5.67|P-value:1.61E-8 ID:SGTC_176|Compound:1486-1328|FD-Score:-4.34|P-value:1.12E-5 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.39|P-value:5.79E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:4.35|P-value:1.46E-5 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.62|P-value:2.58E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:3.43|P-value:5.07E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:6.79|P-value:2.92E-11 ID:SGTC_808|Compound:0711-0032|FD-Score:-3.44|P-value:3.71E-4 ID:SGTC_866|Compound:0495-0101|FD-Score:3.29|P-value:7.94E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:-3.69|P-value:1.50E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:-3.72|P-value:1.37E-4 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.86|P-value:1.07E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.23|P-value:9.76E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:4.43|P-value:1.04E-5 ID:SGTC_1834|Compound:st055981|FD-Score:-3.76|P-value:1.16E-4 ID:SGTC_1839|Compound:st053978|FD-Score:5.50|P-value:5.93E-8 ID:SGTC_1884|Compound:5633444|FD-Score:4.05|P-value:4.90E-5 ID:SGTC_1978|Compound:st071761|FD-Score:4.03|P-value:5.33E-5 ID:SGTC_2059|Compound:5238311|FD-Score:6.92|P-value:1.23E-11 ID:SGTC_2472|Compound:5377652|FD-Score:3.62|P-value:2.53E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.55|P-value:3.34E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-3.44|P-value:3.77E-4 ID:SGTC_3082|Compound:9117008|FD-Score:4.14|P-value:3.52E-5

Top fitness defect scores for YDL105W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2059 5238311 6.92 157.0 µM 1.23E-11 1 2.22E-12 2.38
2 SGTC_574 1315-0397 6.79 40.0 µM 2.92E-11 1 5.61E-12 0.99
3 SGTC_1839 st053978 5.50 48.0 µM 5.93E-8 1 1.92E-8 2.08
4 SGTC_1578 josamycin 4.43 24.2 µM 1.04E-5 1 4.77E-6 0.43
5 SGTC_392 N-ethylmaleimide 4.35 8.3 µM 1.46E-5 RNA pol III & RNase P/MRP 10 6.91E-6 0.01
6 SGTC_3082 9117008 4.14 49.5 µM 3.52E-5 2 1.77E-5 0.15
7 SGTC_1884 5633444 4.05 25.0 µM 4.90E-5 redox potentiating 15 2.51E-5 0.08
8 SGTC_1978 st071761 4.03 42.0 µM 5.33E-5 TSC3-RPN4 6 2.75E-5 0.03
9 SGTC_1486 3970-0793 3.86 4.5 µM 1.07E-4 3 5.77E-5 0.17
10 SGTC_5 0352-0601 3.77 109.9 µM 1.50E-4 ERG2 4 8.31E-5 0.51
11 SGTC_60 0986-0278 3.76 202.0 µM 1.54E-4 3 8.58E-5 0.40
12 SGTC_2472 5377652 3.62 49.4 µM 2.53E-4 TRP & mitochondrial translation 15 1.46E-4 0.07
13 SGTC_416 estradiol valerate 3.62 100.0 µM 2.58E-4 7 1.49E-4 0.01
14 SGTC_149 3970-0822 3.60 46.1 µM 2.75E-4 tubulin folding & SWR complex 16 1.59E-4 0.08
15 SGTC_71 0417-1750 3.56 47.8 µM 3.16E-4 Golgi 14 1.84E-4 0.27
16 SGTC_2596 sinensetin 3.55 100.0 µM 3.34E-4 10 1.96E-4 0.00
17 SGTC_13 0199-0087 3.50 65.0 µM 3.93E-4 ERG2 6 2.33E-4 0.05
18 SGTC_173 1273-0061 3.43 2.7 µM 5.01E-4 mitochondrial stress 37 3.02E-4 0.06
19 SGTC_569 4130-2812 3.43 160.0 µM 5.07E-4 amide catabolism 9 3.06E-4 0.10
20 SGTC_390 dec-rvkr-cmk 3.39 16.7 µM 5.79E-4 calcium & mitochondrial duress 17 3.53E-4 0.17

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.100 7.20E-9 YAR027W UIP3 hom Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family
0.089 2.35E-7 YLR291C GCD7 het Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.088 3.44E-7 YEL033W_p MTC7_p hom Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant
0.086 5.37E-7 YBR152W SPP381 het mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
0.084 9.64E-7 YER098W UBP9 hom Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication
0.084 1.28E-6 YPL204W HRR25 het Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
0.083 1.44E-6 YDL146W LDB17 hom Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck
0.083 1.61E-6 YJL038C LOH1 hom Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1
0.080 3.37E-6 YML015C TAF11 het TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors
0.079 4.91E-6 YPR050C_d YPR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
0.079 5.04E-6 YGL078C DBP3 hom RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype
0.079 4.99E-6 YMR017W SPO20 hom Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog
0.077 9.01E-6 YAL032C PRP45 het Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
0.076 9.79E-6 YBR265W TSC10 het 3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
0.076 1.06E-5 YPL228W CET1 het Beta (RNA 5'-triphosphatase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CEG1, a guanylyltransferase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide