HIPHOP chemogenomics database

YDL131W / LYS21

Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YDL131W / LYS21

Click on Significant Values for Screen Details

Top fitness defect scores for YDL131W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2997	9067075	7.44	71.4 µM	2.14E-17	unfolded protein response	8	5.01E-14
2	SGTC_2989	9073649	6.62	71.4 µM	3.68E-14		7	1.78E-11
3	SGTC_418	lividomycin a	4.23	100.0 µM	8.33E-7		8	1.14E-5
4	SGTC_3125	9127534	3.92	49.5 µM	4.64E-6		12	4.46E-5
5	SGTC_2871	9038510	3.91	13.0 µM	4.95E-6		17	4.70E-5
6	SGTC_3313	9133968	3.85	57.0 µM	6.76E-6		8	6.01E-5
7	SGTC_2947	9072639	3.48	7.7 µM	4.15E-5	tubulin folding & SWR complex	62	2.54E-4
8	SGTC_2605	digitonin	3.46	300.0 nM	4.39E-5		47	2.65E-4
9	SGTC_1924	st058459	3.44	75.1 µM	4.84E-5		21	2.87E-4
10	SGTC_1263	0774-1208	3.30	64.8 µM	9.24E-5		79	4.80E-4
11	SGTC_2876	9059903	3.28	26.0 µM	1.01E-4		33	5.16E-4
12	SGTC_3314	9135860	3.09	59.4 µM	2.32E-4		52	9.97E-4
13	SGTC_1740	st038286	3.07	13.2 µM	2.50E-4		69	0.00106
14	SGTC_2429	5902844	3.00	64.4 µM	3.37E-4		83	0.00134
15	SGTC_1253	0550-0034	2.94	176.0 µM	4.40E-4		67	0.00166
16	SGTC_3315	9136395	2.85	68.5 µM	6.23E-4		54	0.00219
17	SGTC_1928	4'-methoxychalcone	2.85	40.3 µM	6.25E-4		48	0.00220
18	SGTC_3156	9099931	2.82	49.5 µM	6.92E-4		55	0.00238
19	SGTC_459	nigericin	2.71	15.3 µM	0.00108		137	0.00340
20	SGTC_1911	st060208	2.68	74.3 µM	0.00118		102	0.00364

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.263	5.89E-54	YGR292W	MAL12	hom	Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
0.253	2.68E-50	YGR136W	LSB1	hom	Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.222	1.17E-38	YDR133C_d	YDR133C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
0.217	6.74E-37	YJR137C	MET5	hom	Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.209	1.79E-34	YFL014W	HSP12	hom	Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.197	1.28E-30	YNL166C	BNI5	hom	Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.183	1.12E-26	YDL176W	YDL176W	hom	Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.178	2.06E-25	YJL171C	YJL171C	hom	GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.177	4.16E-25	YDR459C	PFA5	hom	Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain
0.177	5.67E-25	YGR022C_d	YGR022C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.176	8.95E-25	YJL131C	AIM23	hom	Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss
0.175	1.65E-24	YDL227C	HO	hom	Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p
0.168	1.39E-22	YDL199C_p	YDL199C_p	hom	Putative transporter, member of the sugar porter family
0.166	2.98E-22	YNL218W	MGS1	hom	Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
0.163	2.20E-21	YPR180W	AOS1	het	Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability