YDL133W / SRF1

Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL133W / SRF1

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:4.13|P-value:1.71E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:4.23|P-value:1.24E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:4.27|P-value:1.08E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:6.75|P-value:1.66E-9 ID:SGTC_207|Compound:4185-0010|FD-Score:5.96|P-value:9.25E-8 ID:SGTC_217|Compound:ketoconazole|FD-Score:-3.48|P-value:6.66E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:8.96|P-value:1.58E-15 ID:SGTC_231|Compound:b-estradiol|FD-Score:3.92|P-value:3.50E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.69|P-value:7.22E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:4.09|P-value:2.02E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:5.04|P-value:5.75E-6 ID:SGTC_352|Compound:k048-0007|FD-Score:4.94|P-value:8.59E-6 ID:SGTC_363|Compound:0455-0021|FD-Score:14.30|P-value:3.45E-37 ID:SGTC_397|Compound:arbidol|FD-Score:5.22|P-value:2.67E-6 ID:SGTC_435|Compound:c-PAF|FD-Score:4.98|P-value:7.36E-6 ID:SGTC_455|Compound:paf C-16|FD-Score:3.69|P-value:7.17E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:8.61|P-value:1.81E-14 ID:SGTC_568|Compound:1193-0097|FD-Score:10.70|P-value:2.44E-21 ID:SGTC_637|Compound:1181-0519|FD-Score:14.00|P-value:1.19E-35 ID:SGTC_649|Compound:1123-0311|FD-Score:4.97|P-value:7.46E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:3.94|P-value:3.32E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:12.80|P-value:8.08E-30 ID:SGTC_807|Compound:1315-0151|FD-Score:-3.50|P-value:6.20E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:6.55|P-value:4.96E-9 ID:SGTC_939|Compound:1165-0479|FD-Score:-4.94|P-value:3.21E-6 ID:SGTC_945|Compound:3381-0768|FD-Score:-3.76|P-value:2.73E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:4.30|P-value:9.74E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:6.33|P-value:1.55E-8 ID:SGTC_1254|Compound:0550-0035|FD-Score:-4.68|P-value:9.26E-6 ID:SGTC_1287|Compound:0971-0001|FD-Score:6.09|P-value:5.03E-8 ID:SGTC_1487|Compound:3970-0795|FD-Score:-4.22|P-value:5.49E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:6.82|P-value:1.13E-9 ID:SGTC_1512|Compound:0259-0544|FD-Score:4.32|P-value:8.88E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:15.50|P-value:6.96E-43 ID:SGTC_1712|Compound:st032288|FD-Score:5.85|P-value:1.61E-7 ID:SGTC_1754|Compound:st042065|FD-Score:6.10|P-value:4.91E-8 ID:SGTC_1787|Compound:fluconazole|FD-Score:-7.69|P-value:1.63E-12 ID:SGTC_1937|Compound:st074714|FD-Score:4.52|P-value:4.36E-5 ID:SGTC_2006|Compound:4011487|FD-Score:4.53|P-value:4.12E-5 ID:SGTC_2016|Compound:5121758|FD-Score:4.46|P-value:5.42E-5 ID:SGTC_2021|Compound:4019055|FD-Score:5.86|P-value:1.51E-7 ID:SGTC_2124|Compound:5246320|FD-Score:3.87|P-value:4.16E-4 ID:SGTC_2144|Compound:5628481|FD-Score:4.05|P-value:2.29E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.74|P-value:6.21E-4 ID:SGTC_2398|Compound:5228213|FD-Score:4.75|P-value:1.80E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:8.99|P-value:1.27E-15 ID:SGTC_2790|Compound:5423970|FD-Score:3.95|P-value:3.16E-4 ID:SGTC_3074|Compound:9116018|FD-Score:4.29|P-value:1.01E-4 ID:SGTC_3219|Compound:9131112|FD-Score:10.40|P-value:2.85E-20 ID:SGTC_3311|Compound:9131146|FD-Score:4.96|P-value:8.02E-6 ID:SGTC_3321|Compound:9137793|FD-Score:4.32|P-value:8.92E-5 ID:SGTC_3351|Compound:9150312|FD-Score:3.71|P-value:6.86E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:4.13|P-value:1.71E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:4.23|P-value:1.24E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:4.27|P-value:1.08E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:6.75|P-value:1.66E-9 ID:SGTC_207|Compound:4185-0010|FD-Score:5.96|P-value:9.25E-8 ID:SGTC_217|Compound:ketoconazole|FD-Score:-3.48|P-value:6.66E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:8.96|P-value:1.58E-15 ID:SGTC_231|Compound:b-estradiol|FD-Score:3.92|P-value:3.50E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.69|P-value:7.22E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:4.09|P-value:2.02E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:5.04|P-value:5.75E-6 ID:SGTC_352|Compound:k048-0007|FD-Score:4.94|P-value:8.59E-6 ID:SGTC_363|Compound:0455-0021|FD-Score:14.30|P-value:3.45E-37 ID:SGTC_397|Compound:arbidol|FD-Score:5.22|P-value:2.67E-6 ID:SGTC_435|Compound:c-PAF|FD-Score:4.98|P-value:7.36E-6 ID:SGTC_455|Compound:paf C-16|FD-Score:3.69|P-value:7.17E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:8.61|P-value:1.81E-14 ID:SGTC_568|Compound:1193-0097|FD-Score:10.70|P-value:2.44E-21 ID:SGTC_637|Compound:1181-0519|FD-Score:14.00|P-value:1.19E-35 ID:SGTC_649|Compound:1123-0311|FD-Score:4.97|P-value:7.46E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:3.94|P-value:3.32E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:12.80|P-value:8.08E-30 ID:SGTC_807|Compound:1315-0151|FD-Score:-3.50|P-value:6.20E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:6.55|P-value:4.96E-9 ID:SGTC_939|Compound:1165-0479|FD-Score:-4.94|P-value:3.21E-6 ID:SGTC_945|Compound:3381-0768|FD-Score:-3.76|P-value:2.73E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:4.30|P-value:9.74E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:6.33|P-value:1.55E-8 ID:SGTC_1254|Compound:0550-0035|FD-Score:-4.68|P-value:9.26E-6 ID:SGTC_1287|Compound:0971-0001|FD-Score:6.09|P-value:5.03E-8 ID:SGTC_1487|Compound:3970-0795|FD-Score:-4.22|P-value:5.49E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:6.82|P-value:1.13E-9 ID:SGTC_1512|Compound:0259-0544|FD-Score:4.32|P-value:8.88E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:15.50|P-value:6.96E-43 ID:SGTC_1712|Compound:st032288|FD-Score:5.85|P-value:1.61E-7 ID:SGTC_1754|Compound:st042065|FD-Score:6.10|P-value:4.91E-8 ID:SGTC_1787|Compound:fluconazole|FD-Score:-7.69|P-value:1.63E-12 ID:SGTC_1937|Compound:st074714|FD-Score:4.52|P-value:4.36E-5 ID:SGTC_2006|Compound:4011487|FD-Score:4.53|P-value:4.12E-5 ID:SGTC_2016|Compound:5121758|FD-Score:4.46|P-value:5.42E-5 ID:SGTC_2021|Compound:4019055|FD-Score:5.86|P-value:1.51E-7 ID:SGTC_2124|Compound:5246320|FD-Score:3.87|P-value:4.16E-4 ID:SGTC_2144|Compound:5628481|FD-Score:4.05|P-value:2.29E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.74|P-value:6.21E-4 ID:SGTC_2398|Compound:5228213|FD-Score:4.75|P-value:1.80E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:8.99|P-value:1.27E-15 ID:SGTC_2790|Compound:5423970|FD-Score:3.95|P-value:3.16E-4 ID:SGTC_3074|Compound:9116018|FD-Score:4.29|P-value:1.01E-4 ID:SGTC_3219|Compound:9131112|FD-Score:10.40|P-value:2.85E-20 ID:SGTC_3311|Compound:9131146|FD-Score:4.96|P-value:8.02E-6 ID:SGTC_3321|Compound:9137793|FD-Score:4.32|P-value:8.92E-5 ID:SGTC_3351|Compound:9150312|FD-Score:3.71|P-value:6.86E-4

Top fitness defect scores for YDL133W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1679 4130-1276 15.50 6.7 µM 6.96E-43 Golgi 3 3.51E-54
2 SGTC_363 0455-0021 14.30 3.1 µM 3.45E-37 azole & statin 3 5.68E-47
3 SGTC_637 1181-0519 14.00 7.3 µM 1.19E-35 fatty acid desaturase (OLE1) 10 5.03E-45
4 SGTC_768 0384-0033 12.80 77.3 µM 8.08E-30 fatty acid desaturase (OLE1) 9 1.23E-37
5 SGTC_568 1193-0097 10.70 7.5 µM 2.44E-21 ERAD & cell cycle 20 6.89E-27
6 SGTC_3219 9131112 10.40 49.5 µM 2.85E-20 Golgi 2 1.56E-25
7 SGTC_2584 androsterone 8.99 48.7 µM 1.27E-15 cell wall 22 1.24E-19
8 SGTC_221 trifluoperazine 8.96 10.3 µM 1.58E-15 NEO1-PIK1 73 1.63E-19
9 SGTC_528 3492-0019 8.61 172.0 µM 1.81E-14 calcium & mitochondrial duress 60 3.61E-18
10 SGTC_1495 4469-0029 6.82 237.0 µM 1.13E-9 40 4.51E-12
11 SGTC_86 0417-1630 6.75 471.9 µM 1.66E-9 ubiquinone biosynthesis & proteasome 75 7.34E-12
12 SGTC_863 0466-0191 6.55 129.0 µM 4.96E-9 cell wall 26 2.96E-11
13 SGTC_1161 k007-0610 6.33 110.0 µM 1.55E-8 ERAD & cell cycle 56 1.26E-10
14 SGTC_1754 st042065 6.10 16.1 µM 4.91E-8 NEO1-PIK1 98 5.47E-10
15 SGTC_1287 0971-0001 6.09 34.6 µM 5.03E-8 sphingolipid biosynthesis & PDR1 21 5.65E-10
16 SGTC_207 4185-0010 5.96 4.5 µM 9.25E-8 endomembrane recycling 44 1.23E-9
17 SGTC_2021 4019055 5.86 173.0 µM 1.51E-7 endomembrane recycling 40 2.29E-9
18 SGTC_1712 st032288 5.85 53.4 µM 1.61E-7 calcium & mitochondrial duress 81 2.48E-9
19 SGTC_397 arbidol 5.22 50.0 µM 2.67E-6 cell wall 8 8.89E-8
20 SGTC_351 caspofungin 5.04 25.0 nM 5.75E-6 cell wall 54 2.36E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.376 3.82E-113 YGR060W ERG25 het C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
0.372 6.03E-111 YBL022C PIM1 hom ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
0.370 1.07E-109 YKR031C SPO14 hom Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
0.299 1.65E-70 YLR380W CSR1 hom Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.297 3.00E-69 YBL046W PSY4 hom Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2
0.295 1.94E-68 YNL070W TOM7 hom Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
0.288 3.68E-65 YMR134W ERG29 het Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
0.280 2.18E-61 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.277 3.52E-60 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.275 1.76E-59 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.260 3.77E-53 YKR034W DAL80 hom Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication
0.260 6.24E-53 YLR057W MNL2 hom Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
0.236 1.69E-43 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.233 1.35E-42 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.227 2.48E-40 YLR330W CHS5 hom Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane