YDL138W / RGT2

Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL138W / RGT2

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:3.17|P-value:1.45E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.00|P-value:2.24E-6 ID:SGTC_165|Compound:k038-0023|FD-Score:-3.12|P-value:1.73E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-2.70|P-value:9.58E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:3.81|P-value:6.43E-6 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.18|P-value:1.28E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:2.89|P-value:4.70E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:-3.00|P-value:2.91E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:4.09|P-value:1.40E-6 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.14|P-value:1.52E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.00|P-value:2.35E-6 ID:SGTC_916|Compound:4466-0038|FD-Score:3.12|P-value:1.75E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.75|P-value:8.42E-6 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.04|P-value:2.52E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.26|P-value:9.07E-5 ID:SGTC_1357|Compound:1503-1303|FD-Score:-3.80|P-value:6.31E-6 ID:SGTC_1712|Compound:st032288|FD-Score:2.77|P-value:7.67E-4 ID:SGTC_1820|Compound:st053765|FD-Score:-2.98|P-value:3.10E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.53|P-value:1.05E-7 ID:SGTC_2034|Compound:5197015|FD-Score:3.01|P-value:2.86E-4 ID:SGTC_2323|Compound:6148992|FD-Score:-4.23|P-value:5.97E-7 ID:SGTC_2767|Compound:sibutramine|FD-Score:2.83|P-value:6.03E-4 ID:SGTC_3054|Compound:9094397|FD-Score:-2.90|P-value:4.37E-4 ID:SGTC_3157|Compound:9099274|FD-Score:3.98|P-value:2.66E-6 ID:SGTC_3159|Compound:9100096|FD-Score:-2.83|P-value:5.67E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-2.98|P-value:3.10E-4 ID:SGTC_3330|Compound:9141424|FD-Score:3.05|P-value:2.44E-4 ID:SGTC_3332|Compound:9141069|FD-Score:-3.39|P-value:4.93E-5 ID:SGTC_39|Compound:0986-0283|FD-Score:3.17|P-value:1.45E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.00|P-value:2.24E-6 ID:SGTC_165|Compound:k038-0023|FD-Score:-3.12|P-value:1.73E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-2.70|P-value:9.58E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:3.81|P-value:6.43E-6 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.18|P-value:1.28E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:2.89|P-value:4.70E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:-3.00|P-value:2.91E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:4.09|P-value:1.40E-6 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.14|P-value:1.52E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.00|P-value:2.35E-6 ID:SGTC_916|Compound:4466-0038|FD-Score:3.12|P-value:1.75E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.75|P-value:8.42E-6 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.04|P-value:2.52E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.26|P-value:9.07E-5 ID:SGTC_1357|Compound:1503-1303|FD-Score:-3.80|P-value:6.31E-6 ID:SGTC_1712|Compound:st032288|FD-Score:2.77|P-value:7.67E-4 ID:SGTC_1820|Compound:st053765|FD-Score:-2.98|P-value:3.10E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.53|P-value:1.05E-7 ID:SGTC_2034|Compound:5197015|FD-Score:3.01|P-value:2.86E-4 ID:SGTC_2323|Compound:6148992|FD-Score:-4.23|P-value:5.97E-7 ID:SGTC_2767|Compound:sibutramine|FD-Score:2.83|P-value:6.03E-4 ID:SGTC_3054|Compound:9094397|FD-Score:-2.90|P-value:4.37E-4 ID:SGTC_3157|Compound:9099274|FD-Score:3.98|P-value:2.66E-6 ID:SGTC_3159|Compound:9100096|FD-Score:-2.83|P-value:5.67E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-2.98|P-value:3.10E-4 ID:SGTC_3330|Compound:9141424|FD-Score:3.05|P-value:2.44E-4 ID:SGTC_3332|Compound:9141069|FD-Score:-3.39|P-value:4.93E-5

Top fitness defect scores for YDL138W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2021 4019055 4.53 173.0 µM 1.05E-7 endomembrane recycling 90 2.89E-6
2 SGTC_412 gemfibrozil 4.09 100.0 µM 1.40E-6 DNA intercalators 6 2.13E-5
3 SGTC_915 methyl methanesulfonate (MMS) 4.00 110.0 mM 2.35E-6 DNA damage response 41 3.18E-5
4 SGTC_3157 9099274 3.98 49.5 µM 2.66E-6 20 3.50E-5
5 SGTC_359 0081-0058 3.81 15.9 µM 6.43E-6 60S ribosome export 49 6.91E-5
6 SGTC_39 0986-0283 3.17 58.4 µM 1.45E-4 calcium & mitochondrial duress 71 7.68E-4
7 SGTC_916 4466-0038 3.12 42.0 nM 1.75E-4 cell wall 127 8.90E-4
8 SGTC_3330 9141424 3.05 71.4 µM 2.44E-4 amide catabolism 54 0.00115
9 SGTC_1229 0139-0193 3.04 12.2 µM 2.52E-4 RSC complex & mRNA processing 47 0.00118
10 SGTC_2034 5197015 3.01 53.1 µM 2.86E-4 SWF1 & branched chain AA biosynthesis 62 0.00130
11 SGTC_402 epigallocatechin gallate 2.89 10.0 µM 4.70E-4 90 0.00192
12 SGTC_2767 sibutramine 2.83 71.5 µM 6.03E-4 76 0.00233
13 SGTC_1712 st032288 2.77 53.4 µM 7.67E-4 calcium & mitochondrial duress 294 0.00281
14 SGTC_1434 4073-0095 2.69 163.0 µM 0.00104 60 0.00356
15 SGTC_875 0709-0210 2.66 25.7 µM 0.00118 fatty acid desaturase (OLE1) 105 0.00392
16 SGTC_3125 9127534 2.64 49.5 µM 0.00127 54 0.00416
17 SGTC_2690 usnic acid 2.63 100.0 µM 0.00130 113 0.00422
18 SGTC_151 3013-0144 2.62 2.3 µM 0.00135 iron homeostasis 121 0.00437
19 SGTC_414 piperonyl butoxide 2.62 100.0 µM 0.00137 76 0.00441
20 SGTC_511 blebbistatin 2.61 171.0 µM 0.00143 DNA intercalators 98 0.00456

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.197 8.11E-31 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.193 1.44E-29 YGR149W_p YGR149W_p hom Putative protein of unknown function; predicted to be an integal membrane protein
0.182 2.92E-26 YGR127W_p YGR127W_p hom Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response
0.180 1.08E-25 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.178 2.44E-25 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.177 5.54E-25 YJR087W_d YJR087W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2
0.176 1.04E-24 YLR023C IZH3 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
0.172 1.15E-23 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.170 2.75E-23 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.166 4.59E-22 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.162 3.78E-21 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.161 5.52E-21 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.160 8.81E-21 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.159 1.72E-20 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.159 1.99E-20 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD