YDL189W / RBS1

Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL189W / RBS1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:9.69|P-value:3.70E-24 ID:SGTC_139|Compound:0099-0334|FD-Score:4.10|P-value:9.53E-6 ID:SGTC_159|Compound:3970-0959|FD-Score:6.37|P-value:1.73E-11 ID:SGTC_163|Compound:1327-0036|FD-Score:8.06|P-value:2.72E-17 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.07|P-value:7.80E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.25|P-value:4.01E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.36|P-value:2.28E-4 ID:SGTC_319|Compound:1348-1596|FD-Score:5.16|P-value:4.00E-8 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.22|P-value:3.86E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.93|P-value:2.16E-5 ID:SGTC_761|Compound:k216-0224|FD-Score:4.01|P-value:1.49E-5 ID:SGTC_938|Compound:3126-1755|FD-Score:3.55|P-value:1.07E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:3.86|P-value:2.95E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:3.59|P-value:9.09E-5 ID:SGTC_998|Compound:3996-0085|FD-Score:3.31|P-value:2.79E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.06|P-value:6.97E-4 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.62|P-value:8.20E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.31|P-value:2.84E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:-3.13|P-value:6.27E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:4.26|P-value:4.52E-6 ID:SGTC_1332|Compound:1480-0653|FD-Score:3.07|P-value:6.77E-4 ID:SGTC_1359|Compound:1582-0056|FD-Score:3.46|P-value:1.56E-4 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.16|P-value:4.88E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:3.01|P-value:8.34E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-3.72|P-value:6.10E-5 ID:SGTC_1816|Compound:st051957|FD-Score:-4.16|P-value:8.51E-6 ID:SGTC_1828|Compound:st055811|FD-Score:3.15|P-value:5.02E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.73|P-value:4.97E-5 ID:SGTC_2269|Compound:7935389|FD-Score:3.60|P-value:8.71E-5 ID:SGTC_2276|Compound:7947513|FD-Score:-4.22|P-value:6.55E-6 ID:SGTC_2293|Compound:7624732|FD-Score:-3.27|P-value:3.76E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.61|P-value:8.42E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:-3.03|P-value:9.00E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.98|P-value:1.65E-5 ID:SGTC_2624|Compound:nerol|FD-Score:3.25|P-value:3.51E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-4.89|P-value:2.10E-7 ID:SGTC_2693|Compound:st077581|FD-Score:2.99|P-value:9.05E-4 ID:SGTC_2782|Compound:7736369|FD-Score:-3.01|P-value:9.58E-4 ID:SGTC_2788|Compound:5282734|FD-Score:4.19|P-value:6.25E-6 ID:SGTC_2956|Compound:9076196|FD-Score:-3.36|P-value:2.57E-4 ID:SGTC_2961|Compound:9083290|FD-Score:-4.06|P-value:1.40E-5 ID:SGTC_2962|Compound:9082311|FD-Score:-3.39|P-value:2.32E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:9.69|P-value:3.70E-24 ID:SGTC_139|Compound:0099-0334|FD-Score:4.10|P-value:9.53E-6 ID:SGTC_159|Compound:3970-0959|FD-Score:6.37|P-value:1.73E-11 ID:SGTC_163|Compound:1327-0036|FD-Score:8.06|P-value:2.72E-17 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.07|P-value:7.80E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.25|P-value:4.01E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.36|P-value:2.28E-4 ID:SGTC_319|Compound:1348-1596|FD-Score:5.16|P-value:4.00E-8 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.22|P-value:3.86E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.93|P-value:2.16E-5 ID:SGTC_761|Compound:k216-0224|FD-Score:4.01|P-value:1.49E-5 ID:SGTC_938|Compound:3126-1755|FD-Score:3.55|P-value:1.07E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:3.86|P-value:2.95E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:3.59|P-value:9.09E-5 ID:SGTC_998|Compound:3996-0085|FD-Score:3.31|P-value:2.79E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.06|P-value:6.97E-4 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.62|P-value:8.20E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.31|P-value:2.84E-4 ID:SGTC_1268|Compound:0828-0275|FD-Score:-3.13|P-value:6.27E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:4.26|P-value:4.52E-6 ID:SGTC_1332|Compound:1480-0653|FD-Score:3.07|P-value:6.77E-4 ID:SGTC_1359|Compound:1582-0056|FD-Score:3.46|P-value:1.56E-4 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.16|P-value:4.88E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:3.01|P-value:8.34E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-3.72|P-value:6.10E-5 ID:SGTC_1816|Compound:st051957|FD-Score:-4.16|P-value:8.51E-6 ID:SGTC_1828|Compound:st055811|FD-Score:3.15|P-value:5.02E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.73|P-value:4.97E-5 ID:SGTC_2269|Compound:7935389|FD-Score:3.60|P-value:8.71E-5 ID:SGTC_2276|Compound:7947513|FD-Score:-4.22|P-value:6.55E-6 ID:SGTC_2293|Compound:7624732|FD-Score:-3.27|P-value:3.76E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.61|P-value:8.42E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:-3.03|P-value:9.00E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.98|P-value:1.65E-5 ID:SGTC_2624|Compound:nerol|FD-Score:3.25|P-value:3.51E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-4.89|P-value:2.10E-7 ID:SGTC_2693|Compound:st077581|FD-Score:2.99|P-value:9.05E-4 ID:SGTC_2782|Compound:7736369|FD-Score:-3.01|P-value:9.58E-4 ID:SGTC_2788|Compound:5282734|FD-Score:4.19|P-value:6.25E-6 ID:SGTC_2956|Compound:9076196|FD-Score:-3.36|P-value:2.57E-4 ID:SGTC_2961|Compound:9083290|FD-Score:-4.06|P-value:1.40E-5 ID:SGTC_2962|Compound:9082311|FD-Score:-3.39|P-value:2.32E-4

Top fitness defect scores for YDL189W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 9.69 9.0 µM 3.70E-24 cell wall 87 1.63E-22
2 SGTC_163 1327-0036 8.06 7.5 µM 2.72E-17 19 3.87E-16
3 SGTC_159 3970-0959 6.37 50.0 µM 1.73E-11 21 9.46E-11
4 SGTC_319 1348-1596 5.16 45.5 µM 4.00E-8 RSC & ERG11 5 1.25E-7
5 SGTC_1270 0831-1170 4.26 17.3 µM 4.52E-6 7 1.01E-5
6 SGTC_2788 5282734 4.19 71.4 µM 6.25E-6 35 1.37E-5
7 SGTC_139 0099-0334 4.10 718.6 µM 9.53E-6 25 2.02E-5
8 SGTC_761 k216-0224 4.01 480.0 µM 1.49E-5 17 3.06E-5
9 SGTC_2583 asiatic acid 3.98 100.0 µM 1.65E-5 37 3.38E-5
10 SGTC_696 1560-0013 3.93 611.0 µM 2.16E-5 22 4.32E-5
11 SGTC_939 1165-0479 3.86 45.3 µM 2.95E-5 28 5.78E-5
12 SGTC_2156 5649887 3.73 115.0 µM 4.97E-5 RPP1 & pyrimidine depletion 18 9.39E-5
13 SGTC_1183 1254-0324 3.62 198.0 µM 8.20E-5 31 1.49E-4
14 SGTC_2396 5342950 3.61 42.8 µM 8.42E-5 RSC complex & mRNA processing 57 1.53E-4
15 SGTC_2269 7935389 3.60 174.4 µM 8.71E-5 tubulin folding & SWR complex 38 1.58E-4
16 SGTC_996 3970-0799 3.59 1.8 µM 9.09E-5 105 1.64E-4
17 SGTC_938 3126-1755 3.55 342.0 µM 1.07E-4 21 1.91E-4
18 SGTC_1359 1582-0056 3.46 520.0 nM 1.56E-4 33 2.71E-4
19 SGTC_310 1273-0058 3.36 1.1 µM 2.28E-4 mitochondrial stress 306 3.87E-4
20 SGTC_998 3996-0085 3.31 177.0 µM 2.79E-4 46 4.66E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.261 4.01E-53 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.251 2.25E-49 YGR193C PDX1 hom Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.246 2.68E-47 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.243 2.35E-46 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.234 4.36E-43 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.234 6.82E-43 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
0.222 1.51E-38 YNL291C MID1 hom N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer
0.220 6.07E-38 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.214 4.36E-36 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.208 3.45E-34 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.202 3.41E-32 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.199 3.15E-31 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.197 9.26E-31 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.195 4.43E-30 YOR093C_p YOR093C_p hom Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents
0.195 5.18E-30 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1