YDL201W / TRM8

Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL201W / TRM8

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:2.89|P-value:6.55E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.67|P-value:2.29E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.75|P-value:1.50E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:2.83|P-value:8.42E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.02|P-value:3.91E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.58|P-value:3.52E-5 ID:SGTC_272|Compound:doxorubicin|FD-Score:-3.83|P-value:9.85E-6 ID:SGTC_318|Compound:1488-0629|FD-Score:9.03|P-value:5.18E-24 ID:SGTC_343|Compound:4130-2827|FD-Score:3.81|P-value:1.16E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:-2.84|P-value:7.69E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:2.96|P-value:5.02E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:-3.09|P-value:2.92E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.04|P-value:3.62E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:-2.82|P-value:8.27E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:5.03|P-value:1.16E-8 ID:SGTC_750|Compound:4073-0104|FD-Score:-2.94|P-value:5.31E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.85|P-value:9.43E-6 ID:SGTC_1340|Compound:1486-1359|FD-Score:4.61|P-value:1.50E-7 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.69|P-value:2.07E-5 ID:SGTC_1375|Compound:2910-0797|FD-Score:-2.93|P-value:5.45E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:-4.89|P-value:2.56E-8 ID:SGTC_1720|Compound:st037298|FD-Score:-3.13|P-value:2.42E-4 ID:SGTC_2096|Compound:5468907|FD-Score:2.79|P-value:9.65E-4 ID:SGTC_2114|Compound:5491910|FD-Score:2.86|P-value:7.58E-4 ID:SGTC_2449|Compound:5812982|FD-Score:-2.78|P-value:9.62E-4 ID:SGTC_2488|Compound:5268135|FD-Score:2.95|P-value:5.30E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-2.86|P-value:7.25E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.06|P-value:3.41E-4 ID:SGTC_2990|Compound:9074105|FD-Score:-3.13|P-value:2.42E-4 ID:SGTC_2996|Compound:9066192|FD-Score:-2.90|P-value:6.08E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:2.89|P-value:6.55E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.67|P-value:2.29E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.75|P-value:1.50E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:2.83|P-value:8.42E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.02|P-value:3.91E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.58|P-value:3.52E-5 ID:SGTC_272|Compound:doxorubicin|FD-Score:-3.83|P-value:9.85E-6 ID:SGTC_318|Compound:1488-0629|FD-Score:9.03|P-value:5.18E-24 ID:SGTC_343|Compound:4130-2827|FD-Score:3.81|P-value:1.16E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:-2.84|P-value:7.69E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:2.96|P-value:5.02E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:-3.09|P-value:2.92E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.04|P-value:3.62E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:-2.82|P-value:8.27E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:5.03|P-value:1.16E-8 ID:SGTC_750|Compound:4073-0104|FD-Score:-2.94|P-value:5.31E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.85|P-value:9.43E-6 ID:SGTC_1340|Compound:1486-1359|FD-Score:4.61|P-value:1.50E-7 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.69|P-value:2.07E-5 ID:SGTC_1375|Compound:2910-0797|FD-Score:-2.93|P-value:5.45E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:-4.89|P-value:2.56E-8 ID:SGTC_1720|Compound:st037298|FD-Score:-3.13|P-value:2.42E-4 ID:SGTC_2096|Compound:5468907|FD-Score:2.79|P-value:9.65E-4 ID:SGTC_2114|Compound:5491910|FD-Score:2.86|P-value:7.58E-4 ID:SGTC_2449|Compound:5812982|FD-Score:-2.78|P-value:9.62E-4 ID:SGTC_2488|Compound:5268135|FD-Score:2.95|P-value:5.30E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-2.86|P-value:7.25E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.06|P-value:3.41E-4 ID:SGTC_2990|Compound:9074105|FD-Score:-3.13|P-value:2.42E-4 ID:SGTC_2996|Compound:9066192|FD-Score:-2.90|P-value:6.08E-4

Top fitness defect scores for YDL201W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_318 1488-0629 9.03 67.2 µM 5.18E-24 copper-dependent oxidative stress 2 8.81E-20
2 SGTC_612 0302-0167 5.03 22.4 µM 1.16E-8 copper-dependent oxidative stress 11 2.51E-7
3 SGTC_1340 1486-1359 4.61 257.0 µM 1.50E-7 7 2.02E-6
4 SGTC_1338 1486-1223 3.85 76.0 µM 9.43E-6 28 5.91E-5
5 SGTC_343 4130-2827 3.81 29.8 µM 1.16E-5 38 6.98E-5
6 SGTC_1368 1852-0141 3.69 284.0 µM 2.07E-5 ERG2 44 1.12E-4
7 SGTC_57 miconazole 3.67 162.5 nM 2.29E-5 ergosterol depletion effects on membrane 30 1.22E-4
8 SGTC_266 formosanin C 3.58 406.0 nM 3.52E-5 sphingolipid biosynthesis & PDR1 76 1.74E-4
9 SGTC_2808 7946468 3.06 45.5 µM 3.41E-4 75 0.00112
10 SGTC_497 capsazepine 3.04 133.0 µM 3.62E-4 43 0.00117
11 SGTC_255 tunicamycin 3.02 200.5 nM 3.91E-4 unfolded protein response 135 0.00125
12 SGTC_358 0075-0058 2.96 9.4 µM 5.02E-4 132 0.00153
13 SGTC_2488 5268135 2.95 45.7 µM 5.30E-4 108 0.00160
14 SGTC_39 0986-0283 2.89 58.4 µM 6.55E-4 calcium & mitochondrial duress 94 0.00191
15 SGTC_2114 5491910 2.86 81.2 µM 7.58E-4 58 0.00215
16 SGTC_67 0335-0847 2.83 266.0 µM 8.42E-4 ubiquinone biosynthesis & proteasome 203 0.00235
17 SGTC_2096 5468907 2.79 200.0 µM 9.65E-4 51 0.00262
18 SGTC_38 0929-0051 2.70 58.3 µM 0.00137 104 0.00350
19 SGTC_457 grayanotoxin iii 2.69 135.0 µM 0.00141 176 0.00357
20 SGTC_502 l-744,832 2.68 89.3 µM 0.00145 calcium & mitochondrial duress 113 0.00366

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.282 2.74E-62 YDR165W TRM82 hom Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.237 3.34E-44 YGL232W TAN1 hom Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
0.159 1.76E-20 YGL140C_p YGL140C_p hom Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains
0.150 2.70E-18 YDR120C TRM1 hom tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
0.149 3.70E-18 YPL212C PUS1 hom tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA
0.139 5.29E-16 YEL038W UTR4 hom Protein with sequence similarity to 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatases, involved in methionine salvage; found in both the cytoplasm and nucleus
0.132 1.50E-14 YDR223W CRF1 hom Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain
0.126 1.96E-13 YLR003C CMS1 hom Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.120 3.62E-12 YBR187W_p GDT1_p hom Putative protein of unknown function; expression is reduced in a gcr1 null mutant; GFP-fusion protein localizes to the vacuole; expression pattern and physical interactions suggest a possible role in ribosome biogenesis
0.113 5.72E-11 YGR271C-A EFG1 hom Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
0.113 6.63E-11 YKR045C_p YKR045C_p hom Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
0.112 8.07E-11 YKL040C NFU1 hom Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria
0.110 1.42E-10 YLR044C PDC1 hom Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism
0.106 7.89E-10 YDR006C SOK1 hom Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13
0.106 8.64E-10 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation