HIPHOP chemogenomics database

YDL217C / TIM22

Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported

Zygosity: Heterozygous strain

fixedexpanded
Profile for YDL217C / TIM22

Click on Significant Values for Screen Details

Top fitness defect scores for YDL217C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2980	9014342	11.90	25.3 µM	6.85E-36		1	4.48E-33	1.57
2	SGTC_908	3448-7491	8.36	47.7 µM	1.28E-18	mitochondrial stress	1	3.18E-17	1.59
3	SGTC_2928	9011484	8.14	9.8 µM	9.39E-18	heme biosynthesis & mitochondrial translocase	3	1.97E-16	0.33
4	SGTC_1785	bathophenanthroline	6.71	24.0 µM	1.19E-12	heme requiring	6	9.55E-12	0.42
5	SGTC_2418	pentamidine	6.35	31.7 µM	1.67E-11	mitochondrial processes	6	1.08E-10	0.28
6	SGTC_2370	9071495	6.26	200.0 µM	3.05E-11	mitochondrial processes	2	1.88E-10	1.31
7	SGTC_2139	5578692	6.03	56.5 µM	1.52E-10	superoxide	7	8.22E-10	1.35
8	SGTC_2659	sanguinarine	5.60	24.2 µM	2.45E-9		5	1.06E-8	0.80
9	SGTC_3181	9108277	5.38	49.5 µM	9.63E-9	iron homeostasis	4	3.72E-8	0.13
10	SGTC_1347	1493-0251	5.22	600.0 nM	2.44E-8	mitochondrial response to ROS	2	8.76E-8	0.56
11	SGTC_2093	5331342	5.04	194.4 µM	6.92E-8		2	2.28E-7	0.70
12	SGTC_204	1543-0184	5.04	62.4 µM	6.95E-8	TRP & mitochondrial translation	5	2.29E-7	0.47
13	SGTC_63	0099-0242	5.01	26.7 µM	8.35E-8	mitochondrial stress	14	2.71E-7	0.09
14	SGTC_969	1313-0238	4.96	134.0 µM	1.10E-7	mitochondrial processes	12	3.49E-7	0.30
15	SGTC_3208	9114115	4.68	49.5 µM	5.21E-7		4	1.46E-6	0.06
16	SGTC_451	chloral hydrate	4.62	100.0 µM	6.87E-7		13	1.88E-6	0.02
17	SGTC_173	1273-0061	4.55	2.7 µM	1.02E-6	mitochondrial stress	17	2.72E-6	0.05
18	SGTC_2471	5466956	4.29	24.8 µM	3.69E-6	mitochondrial processes	12	8.84E-6	0.05
19	SGTC_3141	9094609	4.27	49.5 µM	4.17E-6	iron homeostasis	11	9.91E-6	0.05
20	SGTC_2256	7954252	4.14	200.0 µM	7.50E-6	mitochondrial processes	9	1.70E-5	0.29

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.222	7.79E-39	YBR192W	RIM2	het	Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.220	3.52E-38	YER066W_p	RRT13_p	hom	Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
0.198	5.46E-31	YPL266W	DIM1	het	Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
0.191	6.05E-29	YLR395C	COX8	hom	Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
0.187	7.03E-28	YGL146C_p	RRT6_p	hom	Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins
0.175	1.88E-24	YGR015C_p	YGR015C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
0.173	5.41E-24	YMR113W	FOL3	het	Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
0.149	3.13E-18	YNL220W	ADE12	hom	Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence
0.148	7.29E-18	YMR203W	TOM40	het	Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
0.144	5.74E-17	YGL068W	MNP1	het	Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
0.143	6.54E-17	YJL043W_p	YJL043W_p	hom	Putative protein of unknown function; YJL043W is a non-essential gene
0.132	1.48E-14	YEL049W	PAU2	hom	Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.131	2.36E-14	YOR300W_d	YOR300W_d	hom	Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
0.128	9.71E-14	YKL195W	MIA40	het	Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
0.128	1.06E-13	YKR079C	TRZ1	het	tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2