HIPHOP chemogenomics database

YDL240W / LRG1

Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis

Zygosity: Homozygous strain

fixedexpanded
Profile for YDL240W / LRG1

Click on Significant Values for Screen Details

Top fitness defect scores for YDL240W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1871	st057448	7.77	67.3 µM	3.10E-15		1	4.05E-15
2	SGTC_1966	st070145	6.87	16.1 µM	2.67E-12		4	3.24E-12
3	SGTC_1962	st076595	6.27	64.5 µM	1.53E-10	redox potentiating	5	1.78E-10
4	SGTC_1638	st008370	5.35	60.6 µM	4.00E-8	ergosterol biosynthesis	10	4.40E-8
5	SGTC_1637	st007924	5.33	17.4 µM	4.47E-8		8	4.92E-8
6	SGTC_3191	9110352	5.03	49.5 µM	2.27E-7		1	2.45E-7
7	SGTC_1001	4048-4355	4.82	44.4 µM	6.67E-7	copper-dependent oxidative stress	20	7.14E-7
8	SGTC_1862	st057513	4.70	3.8 µM	1.24E-6		3	1.32E-6
9	SGTC_112	phenothiazine	4.28	287.2 µM	9.10E-6	unfolded protein response	9	9.51E-6
10	SGTC_1865	st057639	4.19	52.2 µM	1.35E-5		8	1.41E-5
11	SGTC_2756	carbadox	4.08	75.1 µM	2.15E-5		53	2.23E-5
12	SGTC_2675	theaflavin digallate	4.08	100.0 µM	2.15E-5	iron homeostasis	29	2.23E-5
13	SGTC_2366	9070441	3.96	60.6 µM	3.68E-5		29	3.81E-5
14	SGTC_3106	9121286	3.93	49.5 µM	4.20E-5		12	4.33E-5
15	SGTC_518	1021-0119	3.90	62.6 µM	4.68E-5	ERG2	41	4.82E-5
16	SGTC_206	4546-0025	3.68	72.0 µM	1.15E-4	NEO1	87	1.17E-4
17	SGTC_1789	tunicamycin	3.65	25.0 nM	1.29E-4	unfolded protein response	60	1.32E-4
18	SGTC_2296	7971656	3.65	126.5 µM	1.29E-4		52	1.32E-4
19	SGTC_837	0091-0265	3.64	12.5 µM	1.35E-4	ubiquinone biosynthesis & proteasome	32	1.38E-4
20	SGTC_519	k048-0011	3.41	62.8 µM	3.25E-4		37	3.30E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.130	4.48E-14	YNL219C	ALG9	hom	Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.119	5.00E-12	YHL001W	RPL14B	hom	Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.116	1.95E-11	YGR206W	MVB12	hom	ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.107	4.33E-10	YDR319C_p	YFT2_p	hom	Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens
0.102	3.02E-9	YDR516C	EMI2	hom	Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.098	1.50E-8	YOR067C	ALG8	hom	Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p
0.096	2.53E-8	YLR417W	VPS36	hom	Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.092	1.04E-7	YBR012C_d	YBR012C_d	hom	Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p
0.090	1.69E-7	YBR084C-A	RPL19A	hom	Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication
0.090	1.87E-7	YNL242W	ATG2	hom	Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.090	1.93E-7	YLR307W	CDA1	hom	Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
0.088	3.07E-7	YPL120W	VPS30	hom	Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.088	3.14E-7	YNL067W	RPL9B	hom	Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.085	7.07E-7	YDL053C	PBP4	hom	Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress
0.085	7.23E-7	YOR002W	ALG6	hom	Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease