HIPHOP chemogenomics database

YDR002W / YRB1

Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1

Zygosity: Heterozygous strain

fixedexpanded
Profile for YDR002W / YRB1

Click on Significant Values for Screen Details

Top fitness defect scores for YDR002W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_168	3970-0862	7.84	530.5 µM	5.96E-14		9	2.20E-15	0.23
2	SGTC_1783	st039459	5.19	87.5 µM	4.51E-7	Golgi	4	1.06E-7	0.12
3	SGTC_1804	st050076	4.85	49.1 µM	2.22E-6		2	6.25E-7	0.72
4	SGTC_81	0411-0046	4.68	10.0 µM	4.74E-6		30	1.45E-6	0.10
5	SGTC_951	1171-0579	4.64	82.7 µM	5.65E-6	cell wall	15	1.77E-6	0.01
6	SGTC_3210	9128157	4.41	49.5 µM	1.49E-5		8	5.20E-6	0.05
7	SGTC_1071	trifluoperazine	4.02	6.3 µM	6.98E-5		8	2.90E-5	0.41
8	SGTC_1711	st032263	3.95	62.8 µM	9.08E-5		3	3.89E-5	0.54
9	SGTC_1150	3909-7983	3.85	10.9 µM	1.34E-4		5	5.99E-5	0.16
10	SGTC_2588	indole-3-carbinol	3.80	100.0 µM	1.58E-4		9	7.18E-5	0.02
11	SGTC_1063	disulfiram	3.77	1.8 µM	1.80E-4		10	8.32E-5	0.08
12	SGTC_2338	7999974	3.75	200.0 µM	1.94E-4		10	9.01E-5	0.01
13	SGTC_96	0929-0093	3.60	37.3 µM	3.27E-4	calcium & mitochondrial duress	6	1.61E-4	0.21
14	SGTC_1512	0259-0544	3.56	12.2 µM	3.73E-4		8	1.86E-4	0.20
15	SGTC_947	3447-0010	3.55	211.0 µM	3.90E-4		6	1.96E-4	0.13
16	SGTC_1857	st056007	3.52	79.3 µM	4.20E-4		3	2.13E-4	0.25
17	SGTC_960	1222-0186	3.49	7.7 µM	4.76E-4		14	2.45E-4	0.06
18	SGTC_2534	helenine	3.48	20.1 µM	4.82E-4	RSC complex & mRNA processing	28	2.48E-4	0.12
19	SGTC_1093	fluphenazine	3.46	8.0 µM	5.16E-4		9	2.68E-4	0.16
20	SGTC_904	1141-0088	3.33	396.0 µM	7.89E-4		5	4.28E-4	0.15

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.200	1.41E-31	YJL101C	GSH1	hom	Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.154	2.58E-19	YOL049W	GSH2	hom	Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
0.136	3.71E-15	YDR088C	SLU7	het	RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.131	2.53E-14	YFL033C	RIM15	hom	Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.124	5.31E-13	YDR005C	MAF1	hom	Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.116	1.81E-11	YDR189W	SLY1	het	Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
0.110	1.97E-10	YDL108W	KIN28	het	Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters
0.103	2.03E-9	YHR202W_p	YHR202W_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization
0.098	1.46E-8	YHR205W	SCH9	hom	AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.096	2.34E-8	YBR290W	BSD2	hom	Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification
0.096	2.76E-8	YBR008C	FLR1	hom	Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress
0.093	7.39E-8	YDR069C	DOA4	hom	Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.089	2.75E-7	YIL026C	IRR1	het	Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
0.088	3.00E-7	YBR193C	MED8	het	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.088	3.98E-7	YNL181W_p	YNL181W_p	het	Putative oxidoreductase; required for cell viability