YDR005C / MAF1

Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR005C / MAF1

Click on Significant Values for Screen Details ID:SGTC_59|Compound:0330-0160|FD-Score:6.73|P-value:3.81E-10 ID:SGTC_87|Compound:0100-0052|FD-Score:4.90|P-value:3.73E-6 ID:SGTC_159|Compound:3970-0959|FD-Score:11.10|P-value:1.57E-24 ID:SGTC_163|Compound:1327-0036|FD-Score:7.99|P-value:1.40E-13 ID:SGTC_166|Compound:k072-0058|FD-Score:4.61|P-value:1.22E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:14.40|P-value:1.04E-39 ID:SGTC_213|Compound:1246-0773|FD-Score:3.40|P-value:9.19E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:5.85|P-value:4.38E-8 ID:SGTC_615|Compound:k087-0205|FD-Score:4.72|P-value:7.97E-6 ID:SGTC_734|Compound:2023-0417|FD-Score:4.44|P-value:2.44E-5 ID:SGTC_743|Compound:1275-0778|FD-Score:4.20|P-value:6.15E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:3.45|P-value:8.02E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:-3.47|P-value:8.06E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:-3.54|P-value:6.55E-4 ID:SGTC_993|Compound:3909-8757|FD-Score:4.24|P-value:5.21E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:6.01|P-value:2.00E-8 ID:SGTC_1170|Compound:0310-0127|FD-Score:-3.75|P-value:3.28E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:4.07|P-value:9.83E-5 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.38|P-value:9.80E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:5.53|P-value:2.19E-7 ID:SGTC_1675|Compound:st018485|FD-Score:3.99|P-value:1.29E-4 ID:SGTC_1732|Compound:st034308|FD-Score:3.84|P-value:2.18E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.79|P-value:2.62E-4 ID:SGTC_2057|Compound:5236571|FD-Score:-3.70|P-value:3.90E-4 ID:SGTC_2149|Compound:5554786|FD-Score:4.38|P-value:3.07E-5 ID:SGTC_2152|Compound:5568786|FD-Score:3.44|P-value:8.14E-4 ID:SGTC_2159|Compound:5658611|FD-Score:4.63|P-value:1.13E-5 ID:SGTC_2277|Compound:7947813|FD-Score:-5.56|P-value:2.12E-7 ID:SGTC_2404|Compound:5161160|FD-Score:3.85|P-value:2.12E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.39|P-value:9.55E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:-3.62|P-value:5.00E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.57|P-value:5.83E-4 ID:SGTC_3124|Compound:9127567|FD-Score:3.76|P-value:2.92E-4 ID:SGTC_3131|Compound:9127911|FD-Score:4.81|P-value:5.48E-6 ID:SGTC_59|Compound:0330-0160|FD-Score:6.73|P-value:3.81E-10 ID:SGTC_87|Compound:0100-0052|FD-Score:4.90|P-value:3.73E-6 ID:SGTC_159|Compound:3970-0959|FD-Score:11.10|P-value:1.57E-24 ID:SGTC_163|Compound:1327-0036|FD-Score:7.99|P-value:1.40E-13 ID:SGTC_166|Compound:k072-0058|FD-Score:4.61|P-value:1.22E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:14.40|P-value:1.04E-39 ID:SGTC_213|Compound:1246-0773|FD-Score:3.40|P-value:9.19E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:5.85|P-value:4.38E-8 ID:SGTC_615|Compound:k087-0205|FD-Score:4.72|P-value:7.97E-6 ID:SGTC_734|Compound:2023-0417|FD-Score:4.44|P-value:2.44E-5 ID:SGTC_743|Compound:1275-0778|FD-Score:4.20|P-value:6.15E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:3.45|P-value:8.02E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:-3.47|P-value:8.06E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:-3.54|P-value:6.55E-4 ID:SGTC_993|Compound:3909-8757|FD-Score:4.24|P-value:5.21E-5 ID:SGTC_1131|Compound:3454-3128|FD-Score:6.01|P-value:2.00E-8 ID:SGTC_1170|Compound:0310-0127|FD-Score:-3.75|P-value:3.28E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:4.07|P-value:9.83E-5 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.38|P-value:9.80E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:5.53|P-value:2.19E-7 ID:SGTC_1675|Compound:st018485|FD-Score:3.99|P-value:1.29E-4 ID:SGTC_1732|Compound:st034308|FD-Score:3.84|P-value:2.18E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.79|P-value:2.62E-4 ID:SGTC_2057|Compound:5236571|FD-Score:-3.70|P-value:3.90E-4 ID:SGTC_2149|Compound:5554786|FD-Score:4.38|P-value:3.07E-5 ID:SGTC_2152|Compound:5568786|FD-Score:3.44|P-value:8.14E-4 ID:SGTC_2159|Compound:5658611|FD-Score:4.63|P-value:1.13E-5 ID:SGTC_2277|Compound:7947813|FD-Score:-5.56|P-value:2.12E-7 ID:SGTC_2404|Compound:5161160|FD-Score:3.85|P-value:2.12E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.39|P-value:9.55E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:-3.62|P-value:5.00E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.57|P-value:5.83E-4 ID:SGTC_3124|Compound:9127567|FD-Score:3.76|P-value:2.92E-4 ID:SGTC_3131|Compound:9127911|FD-Score:4.81|P-value:5.48E-6

Top fitness defect scores for YDR005C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_168 3970-0862 14.40 530.5 µM 1.04E-39 3 3.00E-47
2 SGTC_159 3970-0959 11.10 50.0 µM 1.57E-24 4 4.88E-29
3 SGTC_163 1327-0036 7.99 7.5 µM 1.40E-13 20 6.48E-16
4 SGTC_59 0330-0160 6.73 1.3 µM 3.81E-10 25 8.31E-12
5 SGTC_1131 3454-3128 6.01 32.3 µM 2.00E-8 5 9.45E-10
6 SGTC_214 4373-3496 5.85 70.0 µM 4.38E-8 14 2.41E-9
7 SGTC_1286 0929-0070 5.53 131.0 µM 2.19E-7 7 1.65E-8
8 SGTC_87 0100-0052 4.90 35.5 µM 3.73E-6 58 4.82E-7
9 SGTC_3131 9127911 4.81 49.5 µM 5.48E-6 12 7.62E-7
10 SGTC_615 k087-0205 4.72 167.0 µM 7.97E-6 8 1.19E-6
11 SGTC_2159 5658611 4.63 73.8 µM 1.13E-5 4 1.80E-6
12 SGTC_166 k072-0058 4.61 166.4 µM 1.22E-5 DNA intercalators 20 1.97E-6
13 SGTC_734 2023-0417 4.44 80.0 µM 2.44E-5 ergosterol biosynthesis 7 4.50E-6
14 SGTC_2149 5554786 4.38 194.8 µM 3.07E-5 cell wall signaling 25 5.94E-6
15 SGTC_993 3909-8757 4.24 62.7 µM 5.21E-5 19 1.11E-5
16 SGTC_743 1275-0778 4.20 158.0 µM 6.15E-5 excess fatty acid 30 1.35E-5
17 SGTC_1184 1269-1718 4.07 139.0 µM 9.83E-5 24 2.36E-5
18 SGTC_1675 st018485 3.99 24.5 µM 1.29E-4 60S ribosome export 27 3.28E-5
19 SGTC_2404 5161160 3.85 200.0 µM 2.12E-4 23 5.90E-5
20 SGTC_1732 st034308 3.84 51.8 µM 2.18E-4 28 6.09E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.278 2.82E-60 YJL101C GSH1 hom Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.248 2.69E-48 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.219 1.31E-37 YOL049W GSH2 hom Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
0.214 6.04E-36 YKL193C SDS22 het Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
0.211 3.14E-35 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.206 1.61E-33 YDR069C DOA4 hom Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.202 3.73E-32 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.200 1.60E-31 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.178 2.99E-25 YJL202C_d YJL202C_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex
0.171 2.45E-23 YJR139C HOM6 hom Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
0.169 6.29E-23 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.164 1.46E-21 YHR025W THR1 hom Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.163 2.37E-21 YGR193C PDX1 hom Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.156 9.36E-20 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.150 2.88E-18 YDL189W RBS1 hom Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain