YDR026C / NSI1

RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR026C / NSI1

Click on Significant Values for Screen Details ID:SGTC_186|Compound:3455-3465|FD-Score:2.88|P-value:9.66E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:3.28|P-value:2.10E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:4.11|P-value:5.32E-6 ID:SGTC_763|Compound:3453-1109|FD-Score:3.35|P-value:1.60E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:2.89|P-value:9.28E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:5.89|P-value:1.75E-10 ID:SGTC_935|Compound:2996-0153|FD-Score:3.27|P-value:2.20E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:5.19|P-value:1.57E-8 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.02|P-value:9.02E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.57|P-value:1.12E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.11|P-value:4.06E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:3.05|P-value:5.12E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:2.91|P-value:8.54E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:-3.28|P-value:3.43E-4 ID:SGTC_1794|Compound:st048918|FD-Score:3.46|P-value:1.02E-4 ID:SGTC_1989|Compound:st071868|FD-Score:2.91|P-value:8.56E-4 ID:SGTC_1994|Compound:st072947|FD-Score:3.30|P-value:1.96E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.63|P-value:5.00E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-3.21|P-value:4.55E-4 ID:SGTC_2113|Compound:5491891|FD-Score:3.43|P-value:1.18E-4 ID:SGTC_2483|Compound:5729050|FD-Score:2.95|P-value:7.42E-4 ID:SGTC_2691|Compound:st077356|FD-Score:3.82|P-value:2.08E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.56|P-value:6.57E-5 ID:SGTC_2804|Compound:7954240|FD-Score:3.14|P-value:3.63E-4 ID:SGTC_2809|Compound:7964133|FD-Score:4.01|P-value:8.74E-6 ID:SGTC_3138|Compound:9097216|FD-Score:-4.05|P-value:1.32E-5 ID:SGTC_3140|Compound:9094243|FD-Score:-3.70|P-value:6.33E-5 ID:SGTC_3307|Compound:9126885|FD-Score:-3.15|P-value:5.70E-4 ID:SGTC_186|Compound:3455-3465|FD-Score:2.88|P-value:9.66E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:3.28|P-value:2.10E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:4.11|P-value:5.32E-6 ID:SGTC_763|Compound:3453-1109|FD-Score:3.35|P-value:1.60E-4 ID:SGTC_822|Compound:1487-0950|FD-Score:2.89|P-value:9.28E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:5.89|P-value:1.75E-10 ID:SGTC_935|Compound:2996-0153|FD-Score:3.27|P-value:2.20E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:5.19|P-value:1.57E-8 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.02|P-value:9.02E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.57|P-value:1.12E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.11|P-value:4.06E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:3.05|P-value:5.12E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:2.91|P-value:8.54E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:-3.28|P-value:3.43E-4 ID:SGTC_1794|Compound:st048918|FD-Score:3.46|P-value:1.02E-4 ID:SGTC_1989|Compound:st071868|FD-Score:2.91|P-value:8.56E-4 ID:SGTC_1994|Compound:st072947|FD-Score:3.30|P-value:1.96E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.63|P-value:5.00E-5 ID:SGTC_2005|Compound:4024620|FD-Score:-3.21|P-value:4.55E-4 ID:SGTC_2113|Compound:5491891|FD-Score:3.43|P-value:1.18E-4 ID:SGTC_2483|Compound:5729050|FD-Score:2.95|P-value:7.42E-4 ID:SGTC_2691|Compound:st077356|FD-Score:3.82|P-value:2.08E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.56|P-value:6.57E-5 ID:SGTC_2804|Compound:7954240|FD-Score:3.14|P-value:3.63E-4 ID:SGTC_2809|Compound:7964133|FD-Score:4.01|P-value:8.74E-6 ID:SGTC_3138|Compound:9097216|FD-Score:-4.05|P-value:1.32E-5 ID:SGTC_3140|Compound:9094243|FD-Score:-3.70|P-value:6.33E-5 ID:SGTC_3307|Compound:9126885|FD-Score:-3.15|P-value:5.70E-4

Top fitness defect scores for YDR026C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_934 2950-0684 5.89 338.0 µM 1.75E-10 6 1.94E-9
2 SGTC_938 3126-1755 5.19 342.0 µM 1.57E-8 5 1.08E-7
3 SGTC_756 0508-1999 4.11 28.3 µM 5.32E-6 RPP1 & pyrimidine depletion 19 1.95E-5
4 SGTC_2809 7964133 4.01 13.0 µM 8.74E-6 60S ribosome export 40 3.03E-5
5 SGTC_2691 st077356 3.82 60.2 µM 2.08E-5 32 6.56E-5
6 SGTC_1998 st075797 3.63 76.8 µM 5.00E-5 mitochondrial response to ROS 23 1.43E-4
7 SGTC_2697 mebhydrolin 3.56 72.4 µM 6.57E-5 NEO1 88 1.83E-4
8 SGTC_1794 st048918 3.46 76.9 µM 1.02E-4 16 2.72E-4
9 SGTC_2113 5491891 3.43 107.0 µM 1.18E-4 TSC3-RPN4 44 3.07E-4
10 SGTC_763 3453-1109 3.35 128.0 µM 1.60E-4 38 4.03E-4
11 SGTC_1994 st072947 3.30 40.9 µM 1.96E-4 39 4.85E-4
12 SGTC_659 k018-0002 3.28 107.0 µM 2.10E-4 38 5.15E-4
13 SGTC_935 2996-0153 3.27 430.0 µM 2.20E-4 PDR1 42 5.38E-4
14 SGTC_2804 7954240 3.14 19.5 µM 3.63E-4 68 8.38E-4
15 SGTC_1230 0170-0365 3.11 3.8 µM 4.06E-4 endomembrane recycling 126 9.27E-4
16 SGTC_1315 1237-0019 3.05 3.7 µM 5.12E-4 40 0.00114
17 SGTC_2483 5729050 2.95 27.2 µM 7.42E-4 75 0.00158
18 SGTC_1332 1480-0653 2.91 17.5 µM 8.54E-4 TSC3-RPN4 58 0.00180
19 SGTC_1989 st071868 2.91 50.2 µM 8.56E-4 48 0.00180
20 SGTC_822 1487-0950 2.89 154.0 µM 9.28E-4 55 0.00193

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.161 7.92E-21 YDR089W YDR089W hom Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.142 1.12E-16 YEL063C CAN1 hom Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
0.136 3.07E-15 YFL027C GYP8 hom GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport
0.124 5.58E-13 YDR102C_d YDR102C_d hom Dubious open reading frame; homozygous diploid deletion strain exhibits high budding index
0.122 1.57E-12 YJR041C URB2 het Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis
0.119 4.39E-12 YLR307W CDA1 hom Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
0.118 7.86E-12 YAL059W ECM1 hom Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm
0.115 2.45E-11 YGR207C CIR1 hom Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
0.113 4.56E-11 YMR133W REC114 hom Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division
0.113 5.05E-11 YOR269W PAC1 hom Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly
0.112 8.91E-11 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
0.111 1.33E-10 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.107 5.66E-10 YBL019W APN2 hom Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII
0.106 6.66E-10 YOL008W COQ10 hom Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes
0.106 7.30E-10 YGR216C GPI1 het Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs