HIPHOP chemogenomics database

YDR054C / CDC34

Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain

fixedexpanded
Profile for YDR054C / CDC34

Click on Significant Values for Screen Details

Top fitness defect scores for YDR054C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_126	0417-1624	6.30	672.5 µM	7.93E-14	DNA intercalators	4	1.52E-10	0.81
2	SGTC_113	0099-0221	5.45	2.5 µM	8.78E-11		1	2.56E-8	1.23
3	SGTC_145	3737-0113	5.25	38.4 µM	3.95E-10	DNA intercalators	27	7.72E-8	0.18
4	SGTC_4	0960-0234	4.23	25.7 µM	3.57E-7	Golgi	11	1.14E-5	0.21
5	SGTC_2269	7935389	4.09	174.4 µM	8.57E-7	tubulin folding & SWR complex	7	2.18E-5	0.19
6	SGTC_569	4130-2812	3.98	160.0 µM	1.63E-6	amide catabolism	4	3.50E-5	0.11
7	SGTC_256	clotrimazole	3.91	625.0 nM	2.35E-6	ergosterol depletion effects on membrane	22	4.59E-5	0.04
8	SGTC_2444	5485484	3.88	36.4 µM	2.79E-6		9	5.21E-5	0.14
9	SGTC_280	3448-3607	3.75	3.0 µM	5.61E-6	60S ribosome export	5	8.72E-5	0.19
10	SGTC_2278	7948257	3.51	134.3 µM	2.01E-5		5	2.24E-4	0.06
11	SGTC_1215	3852-0577	3.47	11.2 µM	2.44E-5		11	2.59E-4	0.09
12	SGTC_125	0133-0007	3.47	11.8 µM	2.46E-5	DNA intercalators	11	2.60E-4	0.01
13	SGTC_2566	crystal violet carbinol	3.46	226.0 nM	2.57E-5		28	2.69E-4	0.06
14	SGTC_2200	6600604	3.36	21.9 µM	4.34E-5	tubulin folding & SWR complex	9	3.96E-4	0.14
15	SGTC_62	0180-0423	3.34	3.2 µM	4.62E-5	60S ribosome export	21	4.15E-4	0.04
16	SGTC_785	1270-0111	3.33	65.3 µM	5.04E-5		14	4.42E-4	0.00
17	SGTC_214	4373-3496	3.28	70.0 µM	6.16E-5		29	5.13E-4	0.05
18	SGTC_1775	st081588	3.28	35.2 µM	6.28E-5	60S ribosome export	18	5.20E-4	0.04
19	SGTC_144	4161-2749	3.26	112.4 µM	6.82E-5	DNA intercalators	28	5.53E-4	0.03
20	SGTC_1676	st018489	3.24	23.4 µM	7.51E-5	60S ribosome export	11	5.95E-4	0.35

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.236	9.98E-44	YHR020W	YHR020W	het	Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
0.201	8.87E-32	YEL064C	AVT2	hom	Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.178	3.44E-25	YMR262W_p	YMR262W_p	hom	Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.171	1.90E-23	YDL102W	POL3	het	Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
0.166	4.32E-22	YGL002W	ERP6	hom	Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
0.164	1.27E-21	YMR047C	NUP116	het	FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p)
0.160	1.22E-20	YMR168C	CEP3	het	Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.157	4.90E-20	YGR070W	ROM1	hom	GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
0.151	1.82E-18	YNL163C	RIA1	het	Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes
0.150	2.05E-18	YMR030W	RSF1	hom	Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
0.150	3.34E-18	YEL017C-A	PMP2	hom	Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
0.148	6.97E-18	YDR401W_d	YDR401W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.144	4.32E-17	YHR149C	SKG6	hom	Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.138	8.12E-16	YGL118C_d	YGL118C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.137	1.32E-15	YOL133W	HRT1	het	RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF