YDR054C / CDC34

Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR054C / CDC34

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:2.79|P-value:5.67E-4 ID:SGTC_4|Compound:0960-0234|FD-Score:4.23|P-value:3.57E-7 ID:SGTC_62|Compound:0180-0423|FD-Score:3.34|P-value:4.62E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:5.45|P-value:8.78E-11 ID:SGTC_115|Compound:0527-0192|FD-Score:2.99|P-value:2.33E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-5.54|P-value:3.53E-11 ID:SGTC_125|Compound:0133-0007|FD-Score:3.47|P-value:2.46E-5 ID:SGTC_126|Compound:0417-1624|FD-Score:6.30|P-value:7.93E-14 ID:SGTC_131|Compound:0141-0277|FD-Score:2.86|P-value:4.09E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:3.26|P-value:6.82E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:5.25|P-value:3.95E-10 ID:SGTC_159|Compound:3970-0959|FD-Score:2.68|P-value:8.80E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:3.28|P-value:6.16E-5 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.91|P-value:2.35E-6 ID:SGTC_269|Compound:exalamide|FD-Score:2.89|P-value:3.67E-4 ID:SGTC_276|Compound:colchicine|FD-Score:-2.65|P-value:8.84E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:3.75|P-value:5.61E-6 ID:SGTC_429|Compound:1112-0049|FD-Score:2.79|P-value:5.46E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:3.17|P-value:1.05E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:3.98|P-value:1.63E-6 ID:SGTC_765|Compound:1319-0117|FD-Score:2.99|P-value:2.34E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.33|P-value:5.04E-5 ID:SGTC_787|Compound:4152-0075|FD-Score:2.94|P-value:2.99E-4 ID:SGTC_1200|Compound:1643-0193|FD-Score:3.10|P-value:1.45E-4 ID:SGTC_1215|Compound:3852-0577|FD-Score:3.47|P-value:2.44E-5 ID:SGTC_1331|Compound:1472-0049|FD-Score:-3.14|P-value:1.03E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.24|P-value:7.56E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:2.76|P-value:6.27E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:2.90|P-value:3.53E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:-2.90|P-value:3.12E-4 ID:SGTC_1503|Compound:4585-0012|FD-Score:2.86|P-value:4.17E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.23|P-value:8.11E-5 ID:SGTC_1554|Compound:st069299|FD-Score:-3.56|P-value:1.33E-5 ID:SGTC_1676|Compound:st018489|FD-Score:3.24|P-value:7.51E-5 ID:SGTC_1713|Compound:st033146|FD-Score:2.79|P-value:5.48E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-3.11|P-value:1.21E-4 ID:SGTC_1775|Compound:st081588|FD-Score:3.28|P-value:6.28E-5 ID:SGTC_1855|Compound:st056195|FD-Score:3.19|P-value:9.40E-5 ID:SGTC_1941|Compound:st076221|FD-Score:-2.71|P-value:6.77E-4 ID:SGTC_2003|Compound:4023581|FD-Score:2.86|P-value:4.16E-4 ID:SGTC_2022|Compound:5136654|FD-Score:2.90|P-value:3.50E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.36|P-value:4.34E-5 ID:SGTC_2265|Compound:7929201|FD-Score:-3.14|P-value:1.07E-4 ID:SGTC_2269|Compound:7935389|FD-Score:4.09|P-value:8.57E-7 ID:SGTC_2273|Compound:7815444|FD-Score:3.14|P-value:1.18E-4 ID:SGTC_2278|Compound:7948257|FD-Score:3.51|P-value:2.01E-5 ID:SGTC_2346|Compound:9031566|FD-Score:2.68|P-value:8.54E-4 ID:SGTC_2362|Compound:9071156|FD-Score:2.81|P-value:5.03E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.88|P-value:2.79E-6 ID:SGTC_2483|Compound:5729050|FD-Score:3.07|P-value:1.69E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.46|P-value:2.57E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:-5.58|P-value:2.55E-11 ID:SGTC_2933|Compound:9026794|FD-Score:2.96|P-value:2.72E-4 ID:SGTC_2964|Compound:9083345|FD-Score:2.80|P-value:5.31E-4 ID:SGTC_3008|Compound:9078912|FD-Score:-2.68|P-value:7.87E-4 ID:SGTC_3111|Compound:9122088|FD-Score:2.65|P-value:9.81E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.03|P-value:1.98E-4 ID:SGTC_3203|Compound:9113543|FD-Score:-2.64|P-value:9.10E-4 ID:SGTC_3|Compound:0833-0587|FD-Score:2.79|P-value:5.67E-4 ID:SGTC_4|Compound:0960-0234|FD-Score:4.23|P-value:3.57E-7 ID:SGTC_62|Compound:0180-0423|FD-Score:3.34|P-value:4.62E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:5.45|P-value:8.78E-11 ID:SGTC_115|Compound:0527-0192|FD-Score:2.99|P-value:2.33E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-5.54|P-value:3.53E-11 ID:SGTC_125|Compound:0133-0007|FD-Score:3.47|P-value:2.46E-5 ID:SGTC_126|Compound:0417-1624|FD-Score:6.30|P-value:7.93E-14 ID:SGTC_131|Compound:0141-0277|FD-Score:2.86|P-value:4.09E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:3.26|P-value:6.82E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:5.25|P-value:3.95E-10 ID:SGTC_159|Compound:3970-0959|FD-Score:2.68|P-value:8.80E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:3.28|P-value:6.16E-5 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.91|P-value:2.35E-6 ID:SGTC_269|Compound:exalamide|FD-Score:2.89|P-value:3.67E-4 ID:SGTC_276|Compound:colchicine|FD-Score:-2.65|P-value:8.84E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:3.75|P-value:5.61E-6 ID:SGTC_429|Compound:1112-0049|FD-Score:2.79|P-value:5.46E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:3.17|P-value:1.05E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:3.98|P-value:1.63E-6 ID:SGTC_765|Compound:1319-0117|FD-Score:2.99|P-value:2.34E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.33|P-value:5.04E-5 ID:SGTC_787|Compound:4152-0075|FD-Score:2.94|P-value:2.99E-4 ID:SGTC_1200|Compound:1643-0193|FD-Score:3.10|P-value:1.45E-4 ID:SGTC_1215|Compound:3852-0577|FD-Score:3.47|P-value:2.44E-5 ID:SGTC_1331|Compound:1472-0049|FD-Score:-3.14|P-value:1.03E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.24|P-value:7.56E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:2.76|P-value:6.27E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:2.90|P-value:3.53E-4 ID:SGTC_1473|Compound:k844-0725|FD-Score:-2.90|P-value:3.12E-4 ID:SGTC_1503|Compound:4585-0012|FD-Score:2.86|P-value:4.17E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.23|P-value:8.11E-5 ID:SGTC_1554|Compound:st069299|FD-Score:-3.56|P-value:1.33E-5 ID:SGTC_1676|Compound:st018489|FD-Score:3.24|P-value:7.51E-5 ID:SGTC_1713|Compound:st033146|FD-Score:2.79|P-value:5.48E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-3.11|P-value:1.21E-4 ID:SGTC_1775|Compound:st081588|FD-Score:3.28|P-value:6.28E-5 ID:SGTC_1855|Compound:st056195|FD-Score:3.19|P-value:9.40E-5 ID:SGTC_1941|Compound:st076221|FD-Score:-2.71|P-value:6.77E-4 ID:SGTC_2003|Compound:4023581|FD-Score:2.86|P-value:4.16E-4 ID:SGTC_2022|Compound:5136654|FD-Score:2.90|P-value:3.50E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.36|P-value:4.34E-5 ID:SGTC_2265|Compound:7929201|FD-Score:-3.14|P-value:1.07E-4 ID:SGTC_2269|Compound:7935389|FD-Score:4.09|P-value:8.57E-7 ID:SGTC_2273|Compound:7815444|FD-Score:3.14|P-value:1.18E-4 ID:SGTC_2278|Compound:7948257|FD-Score:3.51|P-value:2.01E-5 ID:SGTC_2346|Compound:9031566|FD-Score:2.68|P-value:8.54E-4 ID:SGTC_2362|Compound:9071156|FD-Score:2.81|P-value:5.03E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.88|P-value:2.79E-6 ID:SGTC_2483|Compound:5729050|FD-Score:3.07|P-value:1.69E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.46|P-value:2.57E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:-5.58|P-value:2.55E-11 ID:SGTC_2933|Compound:9026794|FD-Score:2.96|P-value:2.72E-4 ID:SGTC_2964|Compound:9083345|FD-Score:2.80|P-value:5.31E-4 ID:SGTC_3008|Compound:9078912|FD-Score:-2.68|P-value:7.87E-4 ID:SGTC_3111|Compound:9122088|FD-Score:2.65|P-value:9.81E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.03|P-value:1.98E-4 ID:SGTC_3203|Compound:9113543|FD-Score:-2.64|P-value:9.10E-4

Top fitness defect scores for YDR054C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_126 0417-1624 6.30 672.5 µM 7.93E-14 DNA intercalators 4 1.52E-10 0.81
2 SGTC_113 0099-0221 5.45 2.5 µM 8.78E-11 1 2.56E-8 1.23
3 SGTC_145 3737-0113 5.25 38.4 µM 3.95E-10 DNA intercalators 27 7.72E-8 0.18
4 SGTC_4 0960-0234 4.23 25.7 µM 3.57E-7 Golgi 11 1.14E-5 0.21
5 SGTC_2269 7935389 4.09 174.4 µM 8.57E-7 tubulin folding & SWR complex 7 2.18E-5 0.19
6 SGTC_569 4130-2812 3.98 160.0 µM 1.63E-6 amide catabolism 4 3.50E-5 0.11
7 SGTC_256 clotrimazole 3.91 625.0 nM 2.35E-6 ergosterol depletion effects on membrane 22 4.59E-5 0.04
8 SGTC_2444 5485484 3.88 36.4 µM 2.79E-6 9 5.21E-5 0.14
9 SGTC_280 3448-3607 3.75 3.0 µM 5.61E-6 60S ribosome export 5 8.72E-5 0.19
10 SGTC_2278 7948257 3.51 134.3 µM 2.01E-5 5 2.24E-4 0.06
11 SGTC_1215 3852-0577 3.47 11.2 µM 2.44E-5 11 2.59E-4 0.09
12 SGTC_125 0133-0007 3.47 11.8 µM 2.46E-5 DNA intercalators 11 2.60E-4 0.01
13 SGTC_2566 crystal violet carbinol 3.46 226.0 nM 2.57E-5 28 2.69E-4 0.06
14 SGTC_2200 6600604 3.36 21.9 µM 4.34E-5 tubulin folding & SWR complex 9 3.96E-4 0.14
15 SGTC_62 0180-0423 3.34 3.2 µM 4.62E-5 60S ribosome export 21 4.15E-4 0.04
16 SGTC_785 1270-0111 3.33 65.3 µM 5.04E-5 14 4.42E-4 0.00
17 SGTC_214 4373-3496 3.28 70.0 µM 6.16E-5 29 5.13E-4 0.05
18 SGTC_1775 st081588 3.28 35.2 µM 6.28E-5 60S ribosome export 18 5.20E-4 0.04
19 SGTC_144 4161-2749 3.26 112.4 µM 6.82E-5 DNA intercalators 28 5.53E-4 0.03
20 SGTC_1676 st018489 3.24 23.4 µM 7.51E-5 60S ribosome export 11 5.95E-4 0.35

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.236 9.98E-44 YHR020W YHR020W het Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
0.201 8.87E-32 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.178 3.44E-25 YMR262W_p YMR262W_p hom Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.171 1.90E-23 YDL102W POL3 het Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
0.166 4.32E-22 YGL002W ERP6 hom Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
0.164 1.27E-21 YMR047C NUP116 het FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p)
0.160 1.22E-20 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.157 4.90E-20 YGR070W ROM1 hom GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
0.151 1.82E-18 YNL163C RIA1 het Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes
0.150 2.05E-18 YMR030W RSF1 hom Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
0.150 3.34E-18 YEL017C-A PMP2 hom Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
0.148 6.97E-18 YDR401W_d YDR401W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.144 4.32E-17 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.138 8.12E-16 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.137 1.32E-15 YOL133W HRT1 het RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF