YDR057W / YOS9

ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR057W / YOS9

Click on Significant Values for Screen Details ID:SGTC_61|Compound:0978-8327|FD-Score:-3.24|P-value:4.09E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:-3.07|P-value:7.62E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.37|P-value:3.36E-6 ID:SGTC_127|Compound:0180-0333|FD-Score:4.98|P-value:1.44E-7 ID:SGTC_143|Compound:4032-1194|FD-Score:4.38|P-value:3.25E-6 ID:SGTC_172|Compound:1112-0046|FD-Score:-3.59|P-value:1.07E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:-3.16|P-value:5.59E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:3.05|P-value:8.31E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:-3.37|P-value:2.48E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.10|P-value:1.16E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:3.62|P-value:9.78E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:4.33|P-value:4.01E-6 ID:SGTC_939|Compound:1165-0479|FD-Score:-3.61|P-value:9.71E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:-3.62|P-value:9.36E-5 ID:SGTC_943|Compound:3253-1379|FD-Score:4.17|P-value:8.56E-6 ID:SGTC_952|Compound:1185-0122|FD-Score:-5.50|P-value:7.08E-9 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.80|P-value:4.39E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.65|P-value:8.57E-5 ID:SGTC_1401|Compound:3807-4679|FD-Score:-4.47|P-value:1.97E-6 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.37|P-value:2.47E-4 ID:SGTC_1962|Compound:st076595|FD-Score:3.15|P-value:5.93E-4 ID:SGTC_2164|Compound:5685496|FD-Score:3.27|P-value:3.75E-4 ID:SGTC_2174|Compound:5790901|FD-Score:-3.06|P-value:7.86E-4 ID:SGTC_2480|Compound:5767542|FD-Score:-3.32|P-value:3.03E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:-3.08|P-value:7.27E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:-3.14|P-value:5.95E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:-3.27|P-value:3.71E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:-3.55|P-value:1.23E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-3.97|P-value:2.12E-5 ID:SGTC_2641|Compound:levomentholum|FD-Score:-3.33|P-value:2.89E-4 ID:SGTC_2692|Compound:st077426|FD-Score:-3.12|P-value:6.30E-4 ID:SGTC_2703|Compound:st077852|FD-Score:4.02|P-value:1.70E-5 ID:SGTC_2835|Compound:9006588|FD-Score:3.78|P-value:4.87E-5 ID:SGTC_2853|Compound:9015042|FD-Score:3.96|P-value:2.28E-5 ID:SGTC_2854|Compound:9024559|FD-Score:3.00|P-value:9.90E-4 ID:SGTC_2858|Compound:9025781|FD-Score:4.08|P-value:1.33E-5 ID:SGTC_3028|Compound:9087778|FD-Score:-3.21|P-value:4.61E-4 ID:SGTC_3088|Compound:9117578|FD-Score:3.27|P-value:3.72E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.81|P-value:3.63E-7 ID:SGTC_3221|Compound:9128209|FD-Score:3.32|P-value:3.16E-4 ID:SGTC_3223|Compound:9129970|FD-Score:3.26|P-value:3.85E-4 ID:SGTC_3304|Compound:9125678|FD-Score:3.01|P-value:9.78E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:-3.24|P-value:4.09E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:-3.07|P-value:7.62E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.37|P-value:3.36E-6 ID:SGTC_127|Compound:0180-0333|FD-Score:4.98|P-value:1.44E-7 ID:SGTC_143|Compound:4032-1194|FD-Score:4.38|P-value:3.25E-6 ID:SGTC_172|Compound:1112-0046|FD-Score:-3.59|P-value:1.07E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:-3.16|P-value:5.59E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:3.05|P-value:8.31E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:-3.37|P-value:2.48E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.10|P-value:1.16E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:3.62|P-value:9.78E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:4.33|P-value:4.01E-6 ID:SGTC_939|Compound:1165-0479|FD-Score:-3.61|P-value:9.71E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:-3.62|P-value:9.36E-5 ID:SGTC_943|Compound:3253-1379|FD-Score:4.17|P-value:8.56E-6 ID:SGTC_952|Compound:1185-0122|FD-Score:-5.50|P-value:7.08E-9 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.80|P-value:4.39E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.65|P-value:8.57E-5 ID:SGTC_1401|Compound:3807-4679|FD-Score:-4.47|P-value:1.97E-6 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.37|P-value:2.47E-4 ID:SGTC_1962|Compound:st076595|FD-Score:3.15|P-value:5.93E-4 ID:SGTC_2164|Compound:5685496|FD-Score:3.27|P-value:3.75E-4 ID:SGTC_2174|Compound:5790901|FD-Score:-3.06|P-value:7.86E-4 ID:SGTC_2480|Compound:5767542|FD-Score:-3.32|P-value:3.03E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:-3.08|P-value:7.27E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:-3.14|P-value:5.95E-4 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:-3.27|P-value:3.71E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:-3.55|P-value:1.23E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-3.97|P-value:2.12E-5 ID:SGTC_2641|Compound:levomentholum|FD-Score:-3.33|P-value:2.89E-4 ID:SGTC_2692|Compound:st077426|FD-Score:-3.12|P-value:6.30E-4 ID:SGTC_2703|Compound:st077852|FD-Score:4.02|P-value:1.70E-5 ID:SGTC_2835|Compound:9006588|FD-Score:3.78|P-value:4.87E-5 ID:SGTC_2853|Compound:9015042|FD-Score:3.96|P-value:2.28E-5 ID:SGTC_2854|Compound:9024559|FD-Score:3.00|P-value:9.90E-4 ID:SGTC_2858|Compound:9025781|FD-Score:4.08|P-value:1.33E-5 ID:SGTC_3028|Compound:9087778|FD-Score:-3.21|P-value:4.61E-4 ID:SGTC_3088|Compound:9117578|FD-Score:3.27|P-value:3.72E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.81|P-value:3.63E-7 ID:SGTC_3221|Compound:9128209|FD-Score:3.32|P-value:3.16E-4 ID:SGTC_3223|Compound:9129970|FD-Score:3.26|P-value:3.85E-4 ID:SGTC_3304|Compound:9125678|FD-Score:3.01|P-value:9.78E-4

Top fitness defect scores for YDR057W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_127 0180-0333 4.98 59.1 µM 1.44E-7 mitochondrial response to ROS 17 3.20E-7
2 SGTC_3210 9128157 4.81 49.5 µM 3.63E-7 27 7.65E-7
3 SGTC_143 4032-1194 4.38 15.7 µM 3.25E-6 47 6.04E-6
4 SGTC_103 0844-0104 4.37 51.8 µM 3.36E-6 ERAD & cell cycle 124 6.22E-6
5 SGTC_839 0122-0028 4.33 230.0 µM 4.01E-6 RSC complex & mRNA processing 29 7.37E-6
6 SGTC_943 3253-1379 4.17 105.0 µM 8.56E-6 23 1.50E-5
7 SGTC_2858 9025781 4.08 9.0 µM 1.33E-5 sphingolipid biosynthesis & PDR1 95 2.29E-5
8 SGTC_2703 st077852 4.02 53.4 µM 1.70E-5 21 2.88E-5
9 SGTC_2853 9015042 3.96 58.4 µM 2.28E-5 23 3.80E-5
10 SGTC_2835 9006588 3.78 71.4 µM 4.87E-5 46 7.77E-5
11 SGTC_1161 k007-0610 3.65 110.0 µM 8.57E-5 ERAD & cell cycle 136 1.32E-4
12 SGTC_568 1193-0097 3.62 7.5 µM 9.78E-5 ERAD & cell cycle 169 1.50E-4
13 SGTC_3221 9128209 3.32 49.5 µM 3.16E-4 31 4.55E-4
14 SGTC_3088 9117578 3.27 49.5 µM 3.72E-4 amide catabolism 19 5.30E-4
15 SGTC_2164 5685496 3.27 200.0 µM 3.75E-4 38 5.35E-4
16 SGTC_3223 9129970 3.26 49.5 µM 3.85E-4 43 5.48E-4
17 SGTC_1962 st076595 3.15 64.5 µM 5.93E-4 redox potentiating 66 8.23E-4
18 SGTC_185 k048-0037 3.05 25.5 µM 8.31E-4 RSC & ERG11 34 0.00113
19 SGTC_3304 9125678 3.01 46.1 µM 9.78E-4 100 0.00132
20 SGTC_2854 9024559 3.00 71.4 µM 9.90E-4 71 0.00134

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.205 3.78E-33 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.200 1.04E-31 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.163 1.82E-21 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.154 3.35E-19 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.147 1.42E-17 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.143 6.68E-17 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.142 1.53E-16 YOR197W MCA1 hom Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization
0.140 3.35E-16 YOR363C PIP2 hom Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
0.133 8.38E-15 YJL002C OST1 het Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
0.133 1.07E-14 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.133 1.22E-14 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.130 5.27E-14 YFR008W FAR7 hom Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
0.128 8.23E-14 YBR058C UBP14 hom Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T
0.126 2.42E-13 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.125 4.09E-13 YKL020C SPT23 hom ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting