YDR069C / DOA4

Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR069C / DOA4

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:3.46|P-value:6.36E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:5.09|P-value:1.02E-6 ID:SGTC_66|Compound:0109-0187|FD-Score:3.46|P-value:6.38E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:4.77|P-value:4.38E-6 ID:SGTC_89|Compound:0302-0250|FD-Score:3.37|P-value:8.75E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:4.84|P-value:3.20E-6 ID:SGTC_149|Compound:3970-0822|FD-Score:6.78|P-value:1.22E-10 ID:SGTC_150|Compound:3970-0969|FD-Score:7.90|P-value:7.84E-14 ID:SGTC_155|Compound:k072-0202|FD-Score:3.46|P-value:6.45E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:7.50|P-value:1.19E-12 ID:SGTC_163|Compound:1327-0036|FD-Score:3.70|P-value:2.87E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:12.60|P-value:1.59E-32 ID:SGTC_172|Compound:1112-0046|FD-Score:3.37|P-value:8.63E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:4.23|P-value:4.03E-5 ID:SGTC_207|Compound:4185-0010|FD-Score:5.20|P-value:6.08E-7 ID:SGTC_212|Compound:3807-4682|FD-Score:3.99|P-value:1.02E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:6.63|P-value:2.90E-10 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:4.24|P-value:3.97E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:6.46|P-value:7.82E-10 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:5.51|P-value:1.38E-7 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:7.35|P-value:3.31E-12 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:8.99|P-value:2.05E-17 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:7.61|P-value:5.62E-13 ID:SGTC_268|Compound:camptothecin|FD-Score:5.27|P-value:4.31E-7 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.69|P-value:6.10E-6 ID:SGTC_364|Compound:0083-0097|FD-Score:-3.54|P-value:4.00E-4 ID:SGTC_486|Compound:niguldipine|FD-Score:4.79|P-value:3.94E-6 ID:SGTC_488|Compound:nitrendipine|FD-Score:5.49|P-value:1.51E-7 ID:SGTC_489|Compound:paxilline|FD-Score:3.90|P-value:1.40E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.49|P-value:1.43E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:3.58|P-value:4.26E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.63|P-value:3.69E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.68|P-value:3.08E-4 ID:SGTC_1085|Compound:doxorubicin|FD-Score:4.00|P-value:9.90E-5 ID:SGTC_1112|Compound:tnp00069|FD-Score:4.37|P-value:2.30E-5 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.43|P-value:7.22E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:5.38|P-value:2.52E-7 ID:SGTC_1228|Compound:0124-0032|FD-Score:5.80|P-value:3.03E-8 ID:SGTC_1365|Compound:1672-3266|FD-Score:5.63|P-value:7.37E-8 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.72|P-value:2.71E-4 ID:SGTC_1428|Compound:4049-0204|FD-Score:6.42|P-value:9.98E-10 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.93|P-value:1.28E-4 ID:SGTC_1655|Compound:st012546|FD-Score:3.52|P-value:5.27E-4 ID:SGTC_1694|Compound:st024011|FD-Score:3.36|P-value:8.83E-4 ID:SGTC_1824|Compound:st053723|FD-Score:3.61|P-value:3.93E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:8.04|P-value:2.81E-14 ID:SGTC_1846|Compound:st056244|FD-Score:3.82|P-value:1.85E-4 ID:SGTC_1923|Compound:st058458|FD-Score:-3.60|P-value:3.23E-4 ID:SGTC_2088|Compound:5278280|FD-Score:-3.61|P-value:3.17E-4 ID:SGTC_2090|Compound:5301565|FD-Score:3.87|P-value:1.59E-4 ID:SGTC_2167|Compound:5876490|FD-Score:3.77|P-value:2.29E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:3.64|P-value:3.50E-4 ID:SGTC_2187|Compound:6125751|FD-Score:3.71|P-value:2.72E-4 ID:SGTC_2200|Compound:6600604|FD-Score:6.56|P-value:4.52E-10 ID:SGTC_2211|Compound:7117706|FD-Score:4.32|P-value:2.88E-5 ID:SGTC_2288|Compound:7946289|FD-Score:-3.81|P-value:1.55E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.52|P-value:5.33E-4 ID:SGTC_2335|Compound:9018011|FD-Score:-3.63|P-value:2.91E-4 ID:SGTC_2460|Compound:5552655|FD-Score:3.39|P-value:8.16E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.98|P-value:1.05E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:6.10|P-value:6.28E-9 ID:SGTC_2585|Compound:alliin|FD-Score:-3.64|P-value:2.86E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:-3.60|P-value:3.32E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.98|P-value:3.57E-11 ID:SGTC_2761|Compound:levamisole|FD-Score:-3.33|P-value:8.01E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:6.84|P-value:8.30E-11 ID:SGTC_2769|Compound:toremifene|FD-Score:3.89|P-value:1.46E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:3.33|P-value:9.69E-4 ID:SGTC_2817|Compound:7995342|FD-Score:3.56|P-value:4.55E-4 ID:SGTC_2981|Compound:9013952|FD-Score:-5.92|P-value:1.18E-8 ID:SGTC_3119|Compound:9125183|FD-Score:3.49|P-value:5.76E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-4.61|P-value:6.70E-6 ID:SGTC_3200|Compound:9114385|FD-Score:3.41|P-value:7.60E-4 ID:SGTC_3214|Compound:9129694|FD-Score:5.13|P-value:8.65E-7 ID:SGTC_3307|Compound:9126885|FD-Score:5.22|P-value:5.53E-7 ID:SGTC_43|Compound:0570-0105|FD-Score:3.46|P-value:6.36E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:5.09|P-value:1.02E-6 ID:SGTC_66|Compound:0109-0187|FD-Score:3.46|P-value:6.38E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:4.77|P-value:4.38E-6 ID:SGTC_89|Compound:0302-0250|FD-Score:3.37|P-value:8.75E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:4.84|P-value:3.20E-6 ID:SGTC_149|Compound:3970-0822|FD-Score:6.78|P-value:1.22E-10 ID:SGTC_150|Compound:3970-0969|FD-Score:7.90|P-value:7.84E-14 ID:SGTC_155|Compound:k072-0202|FD-Score:3.46|P-value:6.45E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:7.50|P-value:1.19E-12 ID:SGTC_163|Compound:1327-0036|FD-Score:3.70|P-value:2.87E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:12.60|P-value:1.59E-32 ID:SGTC_172|Compound:1112-0046|FD-Score:3.37|P-value:8.63E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:4.23|P-value:4.03E-5 ID:SGTC_207|Compound:4185-0010|FD-Score:5.20|P-value:6.08E-7 ID:SGTC_212|Compound:3807-4682|FD-Score:3.99|P-value:1.02E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:6.63|P-value:2.90E-10 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:4.24|P-value:3.97E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:6.46|P-value:7.82E-10 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:5.51|P-value:1.38E-7 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:7.35|P-value:3.31E-12 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:8.99|P-value:2.05E-17 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:7.61|P-value:5.62E-13 ID:SGTC_268|Compound:camptothecin|FD-Score:5.27|P-value:4.31E-7 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.69|P-value:6.10E-6 ID:SGTC_364|Compound:0083-0097|FD-Score:-3.54|P-value:4.00E-4 ID:SGTC_486|Compound:niguldipine|FD-Score:4.79|P-value:3.94E-6 ID:SGTC_488|Compound:nitrendipine|FD-Score:5.49|P-value:1.51E-7 ID:SGTC_489|Compound:paxilline|FD-Score:3.90|P-value:1.40E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.49|P-value:1.43E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:3.58|P-value:4.26E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.63|P-value:3.69E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.68|P-value:3.08E-4 ID:SGTC_1085|Compound:doxorubicin|FD-Score:4.00|P-value:9.90E-5 ID:SGTC_1112|Compound:tnp00069|FD-Score:4.37|P-value:2.30E-5 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.43|P-value:7.22E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:5.38|P-value:2.52E-7 ID:SGTC_1228|Compound:0124-0032|FD-Score:5.80|P-value:3.03E-8 ID:SGTC_1365|Compound:1672-3266|FD-Score:5.63|P-value:7.37E-8 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.72|P-value:2.71E-4 ID:SGTC_1428|Compound:4049-0204|FD-Score:6.42|P-value:9.98E-10 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.93|P-value:1.28E-4 ID:SGTC_1655|Compound:st012546|FD-Score:3.52|P-value:5.27E-4 ID:SGTC_1694|Compound:st024011|FD-Score:3.36|P-value:8.83E-4 ID:SGTC_1824|Compound:st053723|FD-Score:3.61|P-value:3.93E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:8.04|P-value:2.81E-14 ID:SGTC_1846|Compound:st056244|FD-Score:3.82|P-value:1.85E-4 ID:SGTC_1923|Compound:st058458|FD-Score:-3.60|P-value:3.23E-4 ID:SGTC_2088|Compound:5278280|FD-Score:-3.61|P-value:3.17E-4 ID:SGTC_2090|Compound:5301565|FD-Score:3.87|P-value:1.59E-4 ID:SGTC_2167|Compound:5876490|FD-Score:3.77|P-value:2.29E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:3.64|P-value:3.50E-4 ID:SGTC_2187|Compound:6125751|FD-Score:3.71|P-value:2.72E-4 ID:SGTC_2200|Compound:6600604|FD-Score:6.56|P-value:4.52E-10 ID:SGTC_2211|Compound:7117706|FD-Score:4.32|P-value:2.88E-5 ID:SGTC_2288|Compound:7946289|FD-Score:-3.81|P-value:1.55E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.52|P-value:5.33E-4 ID:SGTC_2335|Compound:9018011|FD-Score:-3.63|P-value:2.91E-4 ID:SGTC_2460|Compound:5552655|FD-Score:3.39|P-value:8.16E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.98|P-value:1.05E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:6.10|P-value:6.28E-9 ID:SGTC_2585|Compound:alliin|FD-Score:-3.64|P-value:2.86E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:-3.60|P-value:3.32E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.98|P-value:3.57E-11 ID:SGTC_2761|Compound:levamisole|FD-Score:-3.33|P-value:8.01E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:6.84|P-value:8.30E-11 ID:SGTC_2769|Compound:toremifene|FD-Score:3.89|P-value:1.46E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:3.33|P-value:9.69E-4 ID:SGTC_2817|Compound:7995342|FD-Score:3.56|P-value:4.55E-4 ID:SGTC_2981|Compound:9013952|FD-Score:-5.92|P-value:1.18E-8 ID:SGTC_3119|Compound:9125183|FD-Score:3.49|P-value:5.76E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-4.61|P-value:6.70E-6 ID:SGTC_3200|Compound:9114385|FD-Score:3.41|P-value:7.60E-4 ID:SGTC_3214|Compound:9129694|FD-Score:5.13|P-value:8.65E-7 ID:SGTC_3307|Compound:9126885|FD-Score:5.22|P-value:5.53E-7

Top fitness defect scores for YDR069C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_168 3970-0862 12.60 530.5 µM 1.59E-32 5 8.07E-37
2 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 8.99 4.6 µM 2.05E-17 heat shock/prefoldin 8 1.22E-19
3 SGTC_1844 rapamycin 8.04 4.0 nM 2.81E-14 57 4.51E-16
4 SGTC_150 3970-0969 7.90 77.2 µM 7.84E-14 8 1.45E-15
5 SGTC_243 5hr heat shock (37°C) + splitomicin 7.61 1.0 µM 5.62E-13 heat shock/prefoldin 9 1.36E-14
6 SGTC_159 3970-0959 7.50 50.0 µM 1.19E-12 15 3.20E-14
7 SGTC_241 5hr heat shock (37°C) + cantharidin 7.35 155.4 µM 3.31E-12 cell wall signaling 22 1.02E-13
8 SGTC_2679 rapamycin 6.98 1.0 nM 3.57E-11 61 1.53E-12
9 SGTC_2766 oltipraz 6.84 83.9 µM 8.30E-11 heme requiring 7 3.99E-12
10 SGTC_149 3970-0822 6.78 46.1 µM 1.22E-10 tubulin folding & SWR complex 25 6.21E-12
11 SGTC_214 4373-3496 6.63 70.0 µM 2.90E-10 12 1.66E-11
12 SGTC_2200 6600604 6.56 21.9 µM 4.52E-10 tubulin folding & SWR complex 22 2.74E-11
13 SGTC_239 5hr heat shock (37°C) + mitomycin C 6.46 819.4 µM 7.82E-10 heat shock/prefoldin 10 5.11E-11
14 SGTC_1428 4049-0204 6.42 61.7 µM 9.98E-10 1 6.74E-11
15 SGTC_2582 agaric acid 6.10 100.0 µM 6.28E-9 redox potentiating 155 5.46E-10
16 SGTC_1228 0124-0032 5.80 52.5 µM 3.03E-8 3 3.27E-9
17 SGTC_1365 1672-3266 5.63 55.4 µM 7.37E-8 2 8.97E-9
18 SGTC_240 5hr heat shock (37°C) + Streptovitacin 5.51 300.0 µM 1.38E-7 heat shock/prefoldin 21 1.84E-8
19 SGTC_488 nitrendipine 5.49 139.0 µM 1.51E-7 21 2.02E-8
20 SGTC_1193 1488-0126 5.38 41.5 µM 2.52E-7 9 3.64E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.502 1.86E-213 YLR027C AAT2 hom Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.273 2.36E-58 YLR200W YKE2 hom Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.270 4.59E-57 YNL098C RAS2 hom GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
0.266 2.67E-55 YDR260C SWM1 hom Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.255 3.97E-51 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.252 1.63E-49 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.233 2.57E-42 YJL101C GSH1 hom Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.230 1.44E-41 YCR063W BUD31 hom Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
0.210 1.20E-34 YJR074W MOG1 hom Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p
0.209 2.55E-34 YNR006W VPS27 hom Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
0.206 1.61E-33 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.203 1.75E-32 YJL054W TIM54 het Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
0.198 5.48E-31 YPL084W BRO1 hom Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
0.192 3.18E-29 YOR001W RRP6 hom Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.190 1.45E-28 YDR457W TOM1 hom E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase