HIPHOP chemogenomics database

YDR075W / PPH3

Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR075W / PPH3

Click on Significant Values for Screen Details

Top fitness defect scores for YDR075W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1707	st032323	12.20	6.3 µM	1.11E-40	DNA damage response	6	2.73E-34
2	SGTC_2057	5236571	9.13	38.3 µM	7.86E-24	RPP1 & pyrimidine depletion	1	3.44E-20
3	SGTC_829	1635-0120	6.62	117.0 µM	2.03E-13	DNA damage response	9	1.80E-11
4	SGTC_268	camptothecin	6.51	39.6 µM	4.92E-13	DNA damage response	24	3.77E-11
5	SGTC_1362	1611-0397	5.19	101.0 µM	6.24E-9		4	1.04E-7
6	SGTC_3218	9131285	4.92	49.5 µM	3.49E-8	endomembrane recycling	19	4.41E-7
7	SGTC_1522	st012842	4.86	45.6 µM	4.96E-8	RNA pol III & RNase P/MRP	12	5.92E-7
8	SGTC_1155	4088-0394	4.85	48.2 µM	5.11E-8		10	6.07E-7
9	SGTC_773	4358-1457	4.84	44.6 µM	5.41E-8		23	6.36E-7
10	SGTC_1153	4055-0057	4.59	267.0 µM	2.40E-7		9	2.22E-6
11	SGTC_43	0570-0105	4.56	72.5 µM	2.84E-7	ERG2	66	2.56E-6
12	SGTC_2788	5282734	4.49	71.4 µM	4.27E-7		26	3.61E-6
13	SGTC_1075	aripiprazole	4.44	15.2 µM	5.48E-7		14	4.44E-6
14	SGTC_1866	st057638	4.09	64.0 µM	3.50E-6		11	2.11E-5
15	SGTC_207	4185-0010	4.04	4.5 µM	4.54E-6	endomembrane recycling	84	2.62E-5
16	SGTC_1079	idarubicin	3.91	2.4 µM	8.98E-6	DNA damage response	24	4.66E-5
17	SGTC_1089	terbinafine	3.83	2.2 µM	1.29E-5		15	6.31E-5
18	SGTC_1712	st032288	3.82	53.4 µM	1.40E-5	calcium & mitochondrial duress	195	6.75E-5
19	SGTC_196	k015-0023	3.79	18.1 µM	1.57E-5	PDR1	25	7.45E-5
20	SGTC_8	0354-0001	3.68	70.0 µM	2.65E-5		88	1.16E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.308	1.92E-74	YDL059C	RAD59	hom	Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.268	2.86E-56	YGL175C	SAE2	hom	Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.225	7.39E-40	YMR048W	CSM3	hom	Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
0.217	3.62E-37	YOR191W	ULS1	hom	Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.211	5.27E-35	YDR076W	RAD55	hom	Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.196	1.91E-30	YJL092W	SRS2	hom	DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.192	3.17E-29	YDR004W	RAD57	hom	Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.188	3.89E-28	YDR386W	MUS81	hom	Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.177	4.71E-25	YBR099C_d	YBR099C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.176	8.65E-25	YBR098W	MMS4	hom	Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.172	1.41E-23	YGL163C	RAD54	hom	DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.156	1.20E-19	YNL273W	TOF1	hom	Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase
0.155	1.57E-19	YLR135W	SLX4	hom	Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress
0.136	2.14E-15	YKL175W	ZRT3	hom	Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency
0.134	5.67E-15	YBL088C	TEL1	hom	Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)