YDR085C / AFR1

Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR085C / AFR1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:5.62|P-value:3.78E-9 ID:SGTC_433|Compound:pimozide|FD-Score:3.06|P-value:8.35E-4 ID:SGTC_650|Compound:1328-0195|FD-Score:-3.27|P-value:3.82E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:3.53|P-value:1.45E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:-3.46|P-value:1.85E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:5.39|P-value:1.54E-8 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.68|P-value:7.63E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.07|P-value:8.02E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:3.05|P-value:8.77E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:-3.25|P-value:4.05E-4 ID:SGTC_1777|Compound:st045864|FD-Score:3.34|P-value:3.00E-4 ID:SGTC_1995|Compound:st072814|FD-Score:3.10|P-value:7.16E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.38|P-value:2.60E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.20|P-value:5.04E-4 ID:SGTC_2024|Compound:5144227|FD-Score:-3.41|P-value:2.26E-4 ID:SGTC_2066|Compound:5222451|FD-Score:-5.48|P-value:8.78E-9 ID:SGTC_2386|Compound:9075165|FD-Score:3.52|P-value:1.47E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.42|P-value:2.74E-6 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:5.16|P-value:5.60E-8 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.17|P-value:5.61E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-3.62|P-value:9.67E-5 ID:SGTC_2773|Compound:thioridazine|FD-Score:-3.43|P-value:2.08E-4 ID:SGTC_2784|Compound:7567673|FD-Score:-3.43|P-value:2.05E-4 ID:SGTC_2788|Compound:5282734|FD-Score:-3.27|P-value:3.78E-4 ID:SGTC_2837|Compound:9008476|FD-Score:-3.56|P-value:1.26E-4 ID:SGTC_2852|Compound:9023854|FD-Score:-3.65|P-value:8.79E-5 ID:SGTC_2855|Compound:9016525|FD-Score:4.78|P-value:4.46E-7 ID:SGTC_2859|Compound:9028643|FD-Score:-3.15|P-value:6.01E-4 ID:SGTC_2904|Compound:7225135|FD-Score:3.61|P-value:1.02E-4 ID:SGTC_2909|Compound:7941780|FD-Score:5.74|P-value:1.85E-9 ID:SGTC_2978|Compound:9011784|FD-Score:6.21|P-value:8.73E-11 ID:SGTC_3049|Compound:9092223|FD-Score:3.05|P-value:8.68E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.41|P-value:2.28E-4 ID:SGTC_3124|Compound:9127567|FD-Score:5.11|P-value:7.31E-8 ID:SGTC_3126|Compound:9127922|FD-Score:4.25|P-value:6.39E-6 ID:SGTC_3305|Compound:9127027|FD-Score:-3.38|P-value:2.50E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:5.62|P-value:3.78E-9 ID:SGTC_433|Compound:pimozide|FD-Score:3.06|P-value:8.35E-4 ID:SGTC_650|Compound:1328-0195|FD-Score:-3.27|P-value:3.82E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:3.53|P-value:1.45E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:-3.46|P-value:1.85E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:5.39|P-value:1.54E-8 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.68|P-value:7.63E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.07|P-value:8.02E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:3.05|P-value:8.77E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:-3.25|P-value:4.05E-4 ID:SGTC_1777|Compound:st045864|FD-Score:3.34|P-value:3.00E-4 ID:SGTC_1995|Compound:st072814|FD-Score:3.10|P-value:7.16E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.38|P-value:2.60E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.20|P-value:5.04E-4 ID:SGTC_2024|Compound:5144227|FD-Score:-3.41|P-value:2.26E-4 ID:SGTC_2066|Compound:5222451|FD-Score:-5.48|P-value:8.78E-9 ID:SGTC_2386|Compound:9075165|FD-Score:3.52|P-value:1.47E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.42|P-value:2.74E-6 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:5.16|P-value:5.60E-8 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.17|P-value:5.61E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-3.62|P-value:9.67E-5 ID:SGTC_2773|Compound:thioridazine|FD-Score:-3.43|P-value:2.08E-4 ID:SGTC_2784|Compound:7567673|FD-Score:-3.43|P-value:2.05E-4 ID:SGTC_2788|Compound:5282734|FD-Score:-3.27|P-value:3.78E-4 ID:SGTC_2837|Compound:9008476|FD-Score:-3.56|P-value:1.26E-4 ID:SGTC_2852|Compound:9023854|FD-Score:-3.65|P-value:8.79E-5 ID:SGTC_2855|Compound:9016525|FD-Score:4.78|P-value:4.46E-7 ID:SGTC_2859|Compound:9028643|FD-Score:-3.15|P-value:6.01E-4 ID:SGTC_2904|Compound:7225135|FD-Score:3.61|P-value:1.02E-4 ID:SGTC_2909|Compound:7941780|FD-Score:5.74|P-value:1.85E-9 ID:SGTC_2978|Compound:9011784|FD-Score:6.21|P-value:8.73E-11 ID:SGTC_3049|Compound:9092223|FD-Score:3.05|P-value:8.68E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.41|P-value:2.28E-4 ID:SGTC_3124|Compound:9127567|FD-Score:5.11|P-value:7.31E-8 ID:SGTC_3126|Compound:9127922|FD-Score:4.25|P-value:6.39E-6 ID:SGTC_3305|Compound:9127027|FD-Score:-3.38|P-value:2.50E-4

Top fitness defect scores for YDR085C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2978 9011784 6.21 43.0 µM 8.73E-11 5 2.67E-10
2 SGTC_2909 7941780 5.74 10.5 µM 1.85E-9 2 4.81E-9
3 SGTC_6 0844-0013 5.62 19.8 µM 3.78E-9 21 9.48E-9
4 SGTC_933 2914-0560 5.39 114.0 µM 1.54E-8 14 3.59E-8
5 SGTC_2572 astragaloside iv 5.16 100.0 µM 5.60E-8 7 1.22E-7
6 SGTC_3124 9127567 5.11 49.5 µM 7.31E-8 DNA damage response 6 1.57E-7
7 SGTC_2855 9016525 4.78 58.4 µM 4.46E-7 10 8.73E-7
8 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 4.42 3.7 µM 2.74E-6 55 4.88E-6
9 SGTC_3126 9127922 4.25 49.5 µM 6.39E-6 fatty acid desaturase (OLE1) 27 1.09E-5
10 SGTC_2904 7225135 3.61 42.0 µM 1.02E-4 12 1.51E-4
11 SGTC_823 0417-1665 3.53 178.0 µM 1.45E-4 51 2.10E-4
12 SGTC_2386 9075165 3.52 157.4 µM 1.47E-4 17 2.13E-4
13 SGTC_3080 9117754 3.41 49.5 µM 2.28E-4 34 3.24E-4
14 SGTC_2018 5133068 3.38 143.0 µM 2.60E-4 42 3.66E-4
15 SGTC_1777 st045864 3.34 34.8 µM 3.00E-4 23 4.19E-4
16 SGTC_2021 4019055 3.20 173.0 µM 5.04E-4 endomembrane recycling 193 6.87E-4
17 SGTC_1995 st072814 3.10 61.1 µM 7.16E-4 37 9.59E-4
18 SGTC_433 pimozide 3.06 108.0 µM 8.35E-4 45 0.00111
19 SGTC_3049 9092223 3.05 49.5 µM 8.68E-4 65 0.00115
20 SGTC_1449 4112-4002 3.05 201.0 µM 8.77E-4 RPP1 & pyrimidine depletion 44 0.00116

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.221 2.85E-38 YNL176C_p TDA7_p hom Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele
0.180 9.37E-26 YPL004C LSP1 hom Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
0.168 1.51E-22 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.152 9.98E-19 YKR069W MET1 hom S-adenosyl-L-methionine uroporphyrinogen III transmethylase, involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis
0.147 1.07E-17 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.145 4.11E-17 YJR019C TES1 hom Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
0.140 3.65E-16 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.135 4.44E-15 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.134 6.14E-15 YNL274C GOR1 hom Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
0.134 6.24E-15 YDL018C ERP3 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.131 2.86E-14 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.129 5.52E-14 YDL029W ARP2 het Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
0.129 7.25E-14 YBL029C-A YBL029C-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica
0.124 6.65E-13 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.121 1.76E-12 YDR096W GIS1 hom Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication