HIPHOP chemogenomics database

YDR099W / BMH2

14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR099W / BMH2

Click on Significant Values for Screen Details

Top fitness defect scores for YDR099W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2987	9018338	5.92	71.4 µM	5.65E-9	RPP1 & pyrimidine depletion	3	1.65E-9
2	SGTC_511	blebbistatin	5.48	171.0 µM	6.33E-8	DNA intercalators	6	2.18E-8
3	SGTC_457	grayanotoxin iii	5.09	135.0 µM	4.46E-7		27	1.76E-7
4	SGTC_2326	6164455	4.85	177.6 µM	1.47E-6	mitochondrial processes	7	6.30E-7
5	SGTC_2322	7413319	4.59	38.2 µM	4.67E-6	ERAD & cell cycle	119	2.17E-6
6	SGTC_771	0250-0039	4.57	27.3 µM	5.17E-6		7	2.41E-6
7	SGTC_555	4296-0685	4.32	37.0 µM	1.55E-5	ergosterol biosynthesis	5	7.80E-6
8	SGTC_503	tyrphostin 8	3.96	294.0 µM	6.73E-5	TSC3-RPN4	25	3.75E-5
9	SGTC_2934	9036370	3.73	36.9 µM	1.62E-4		40	9.54E-5
10	SGTC_2364	9055074	3.63	200.0 µM	2.36E-4		27	1.43E-4
11	SGTC_947	3447-0010	3.62	211.0 µM	2.43E-4		22	1.48E-4
12	SGTC_2709	st078560	3.47	56.0 µM	4.19E-4		22	2.64E-4
13	SGTC_842	0271-0008	3.43	11.1 µM	4.83E-4	heme biosynthesis & mitochondrial translocase	34	3.07E-4
14	SGTC_2287	7945887	3.34	168.8 µM	6.56E-4		15	4.26E-4
15	SGTC_2165	5727197	3.25	32.1 µM	8.80E-4		65	5.83E-4
16	SGTC_701	4424-0219	3.23	185.0 µM	9.33E-4	calcium & mitochondrial duress	38	6.21E-4
17	SGTC_1918	oxolan-2-ylmethyl 3-bromobenzoate	3.20	70.1 µM	0.00104		67	6.97E-4
18	SGTC_2621	lithochol-11-enic acid	3.11	100.0 µM	0.00138		71	9.44E-4
19	SGTC_3165	9101544	3.09	49.5 µM	0.00146		33	9.99E-4
20	SGTC_1412	3909-8563	3.07	178.0 µM	0.00155		39	0.00107

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.199	2.56E-31	YGL081W_p	YGL081W_p	hom	Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.180	9.26E-26	YBL036C	YBL036C	hom	Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.175	1.29E-24	YJL135W_d	YJL135W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3
0.171	2.14E-23	YJR019C	TES1	hom	Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
0.165	5.46E-22	YBL086C_p	YBL086C_p	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.163	1.76E-21	YML084W_d	YML084W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.157	7.83E-20	YDL110C	TMA17	hom	Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.150	3.05E-18	YLR205C	HMX1	hom	ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.146	1.66E-17	YDR122W	KIN1	hom	Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p
0.146	1.84E-17	YPL264C_p	YPL264C_p	hom	Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene
0.145	3.71E-17	YGR025W_d	YGR025W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.138	9.89E-16	YOR012W_p	YOR012W_p	hom	Putative protein of unknown function
0.136	2.74E-15	YKL205W	LOS1	hom	Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.134	5.58E-15	YLR016C	PML1	hom	Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
0.132	1.39E-14	YOR238W_p	YOR238W_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm