YDR101C / ARX1

Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR101C / ARX1

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:-3.22|P-value:8.12E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.87|P-value:7.77E-5 ID:SGTC_66|Compound:0109-0187|FD-Score:-3.60|P-value:2.19E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.04|P-value:3.88E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.92|P-value:6.30E-5 ID:SGTC_428|Compound:3448-4153|FD-Score:-3.27|P-value:7.00E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.19|P-value:2.09E-5 ID:SGTC_1219|Compound:1469-0211|FD-Score:-3.63|P-value:1.96E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.01|P-value:5.29E-5 ID:SGTC_1315|Compound:1237-0019|FD-Score:-3.77|P-value:1.16E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:-3.20|P-value:8.63E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:4.44|P-value:8.45E-6 ID:SGTC_1882|Compound:5615643|FD-Score:-3.49|P-value:3.19E-4 ID:SGTC_2061|Compound:5245884|FD-Score:-4.03|P-value:4.11E-5 ID:SGTC_2065|Compound:5221596|FD-Score:-3.57|P-value:2.44E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:3.30|P-value:7.09E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.63|P-value:2.21E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.39|P-value:5.30E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:-4.67|P-value:2.56E-6 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.31|P-value:6.99E-4 ID:SGTC_2414|Compound:st077232|FD-Score:5.25|P-value:1.87E-7 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:5.04|P-value:5.14E-7 ID:SGTC_2686|Compound:alizarin|FD-Score:3.97|P-value:6.04E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:11.70|P-value:3.47E-30 ID:SGTC_3040|Compound:9091894|FD-Score:-3.33|P-value:5.66E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:-3.22|P-value:8.12E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.87|P-value:7.77E-5 ID:SGTC_66|Compound:0109-0187|FD-Score:-3.60|P-value:2.19E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.04|P-value:3.88E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.92|P-value:6.30E-5 ID:SGTC_428|Compound:3448-4153|FD-Score:-3.27|P-value:7.00E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.19|P-value:2.09E-5 ID:SGTC_1219|Compound:1469-0211|FD-Score:-3.63|P-value:1.96E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.01|P-value:5.29E-5 ID:SGTC_1315|Compound:1237-0019|FD-Score:-3.77|P-value:1.16E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:-3.20|P-value:8.63E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:4.44|P-value:8.45E-6 ID:SGTC_1882|Compound:5615643|FD-Score:-3.49|P-value:3.19E-4 ID:SGTC_2061|Compound:5245884|FD-Score:-4.03|P-value:4.11E-5 ID:SGTC_2065|Compound:5221596|FD-Score:-3.57|P-value:2.44E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:3.30|P-value:7.09E-4 ID:SGTC_2394|Compound:5221648|FD-Score:3.63|P-value:2.21E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.39|P-value:5.30E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:-4.67|P-value:2.56E-6 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.31|P-value:6.99E-4 ID:SGTC_2414|Compound:st077232|FD-Score:5.25|P-value:1.87E-7 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:5.04|P-value:5.14E-7 ID:SGTC_2686|Compound:alizarin|FD-Score:3.97|P-value:6.04E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:11.70|P-value:3.47E-30 ID:SGTC_3040|Compound:9091894|FD-Score:-3.33|P-value:5.66E-4

Top fitness defect scores for YDR101C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2688 kanamycin b 11.70 96.7 µM 3.47E-30 calcium & mitochondrial duress 14 6.51E-32
2 SGTC_2414 st077232 5.25 92.9 µM 1.87E-7 6 7.76E-8
3 SGTC_2422 chlorphenesin carbamate 5.04 384.2 µM 5.14E-7 11 2.27E-7
4 SGTC_1680 diphenhydramine 4.44 100.0 µM 8.45E-6 NEO1 19 4.41E-6
5 SGTC_1308 1187-1617 4.01 41.4 µM 5.29E-5 fatty acid desaturase (OLE1) 40 3.08E-5
6 SGTC_2686 alizarin 3.97 95.4 µM 6.04E-5 RPP1 & pyrimidine depletion 30 3.54E-5
7 SGTC_2394 5221648 3.63 200.0 µM 2.21E-4 33 1.40E-4
8 SGTC_2404 5161160 3.39 200.0 µM 5.30E-4 31 3.53E-4
9 SGTC_2408 sulbentine 3.31 18.4 µM 6.99E-4 73 4.74E-4
10 SGTC_2232 chlorophetanol 3.30 41.5 µM 7.09E-4 TRP & mitochondrial translation 173 4.81E-4
11 SGTC_2484 5809818 3.14 12.0 µM 0.00120 41 8.42E-4
12 SGTC_1563 conessine 3.04 56.1 µM 0.00166 50 0.00118
13 SGTC_982 1491-0518 3.03 27.5 µM 0.00169 46 0.00121
14 SGTC_6 0844-0013 3.02 19.8 µM 0.00175 208 0.00125
15 SGTC_625 k213-0058 3.01 121.0 µM 0.00182 translation 200 0.00131
16 SGTC_1684 st024538 3.00 14.6 µM 0.00185 43 0.00133
17 SGTC_1033 k072-0102 3.00 21.3 µM 0.00190 60S ribosome export 67 0.00137
18 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 2.97 3.7 µM 0.00207 214 0.00150
19 SGTC_1442 4112-3290 2.97 3.0 µM 0.00209 55 0.00151
20 SGTC_333 3486-0131 2.96 40.1 µM 0.00212 amide catabolism 38 0.00154

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.272 7.88E-58 YCR075W-A_p YCR075W-A_p hom Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
0.254 1.38E-50 YDR156W RPA14 hom RNA polymerase I subunit A14
0.231 7.22E-42 YBR287W_p YBR287W_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
0.222 1.25E-38 YKL061W_p BLI1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.210 7.88E-35 YOR008C-A_p YOR008C-A_p hom Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres
0.200 1.16E-31 YEL022W GEA2 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.192 2.44E-29 YML019W OST6 hom Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p
0.192 4.53E-29 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.191 4.79E-29 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.187 7.53E-28 YDR417C_d YDR417C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W
0.186 1.40E-27 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.184 5.28E-27 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.181 3.52E-26 YMR099C YMR099C hom Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
0.178 3.66E-25 YEL005C VAB2 hom Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter
0.176 8.02E-25 YDL037C BSC1 hom Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression