YDR139C / RUB1

Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR139C / RUB1

Click on Significant Values for Screen Details ID:SGTC_141|Compound:0443-0274|FD-Score:-3.23|P-value:1.13E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:2.84|P-value:6.23E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.51|P-value:3.17E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.37|P-value:5.73E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:4.91|P-value:1.09E-8 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.69|P-value:4.29E-8 ID:SGTC_167|Compound:3672-0051|FD-Score:-2.98|P-value:3.24E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:2.84|P-value:6.09E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:6.28|P-value:4.16E-13 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.77|P-value:8.26E-6 ID:SGTC_614|Compound:0462-0418|FD-Score:-4.55|P-value:1.01E-7 ID:SGTC_630|Compound:4356-0227|FD-Score:-3.96|P-value:3.08E-6 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.92|P-value:3.83E-6 ID:SGTC_640|Compound:3381-0960|FD-Score:4.74|P-value:3.44E-8 ID:SGTC_652|Compound:0437-0092|FD-Score:-6.36|P-value:1.89E-13 ID:SGTC_697|Compound:0508-1955|FD-Score:2.87|P-value:5.51E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:2.91|P-value:4.69E-4 ID:SGTC_825|Compound:1988-2224|FD-Score:-2.97|P-value:3.35E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:-4.01|P-value:2.32E-6 ID:SGTC_939|Compound:1165-0479|FD-Score:2.93|P-value:4.22E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.21|P-value:1.22E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.74|P-value:9.23E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:-3.23|P-value:1.12E-4 ID:SGTC_1198|Compound:1612-1005|FD-Score:2.77|P-value:8.14E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:3.45|P-value:4.24E-5 ID:SGTC_1630|Compound:st005209|FD-Score:2.85|P-value:5.83E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.08|P-value:2.32E-4 ID:SGTC_1779|Compound:st039427|FD-Score:2.75|P-value:8.92E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.01|P-value:2.34E-6 ID:SGTC_1947|Compound:st076332|FD-Score:-3.15|P-value:1.58E-4 ID:SGTC_2093|Compound:5331342|FD-Score:3.46|P-value:4.13E-5 ID:SGTC_2103|Compound:5378138|FD-Score:3.31|P-value:8.29E-5 ID:SGTC_2165|Compound:5727197|FD-Score:-6.30|P-value:3.00E-13 ID:SGTC_2274|Compound:7947094|FD-Score:-2.84|P-value:5.77E-4 ID:SGTC_2293|Compound:7624732|FD-Score:-4.21|P-value:7.59E-7 ID:SGTC_2298|Compound:7976326|FD-Score:-3.05|P-value:2.46E-4 ID:SGTC_2303|Compound:7774051|FD-Score:4.47|P-value:1.83E-7 ID:SGTC_2354|Compound:9060492|FD-Score:-3.18|P-value:1.41E-4 ID:SGTC_2357|Compound:9067248|FD-Score:-2.74|P-value:8.69E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.19|P-value:1.34E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-3.74|P-value:9.38E-6 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.08|P-value:2.31E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.86|P-value:5.69E-6 ID:SGTC_2457|Compound:5731572|FD-Score:2.97|P-value:3.59E-4 ID:SGTC_2458|Compound:5808907|FD-Score:2.95|P-value:3.91E-4 ID:SGTC_2464|Compound:5717415|FD-Score:-2.81|P-value:6.44E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-4.76|P-value:2.61E-8 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-2.80|P-value:6.71E-4 ID:SGTC_2693|Compound:st077581|FD-Score:3.27|P-value:9.84E-5 ID:SGTC_2732|Compound:terfenadine|FD-Score:-2.75|P-value:8.27E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:2.87|P-value:5.44E-4 ID:SGTC_3003|Compound:9075612|FD-Score:2.82|P-value:6.67E-4 ID:SGTC_3018|Compound:9082602|FD-Score:3.48|P-value:3.73E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.23|P-value:1.13E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:2.84|P-value:6.23E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.51|P-value:3.17E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.37|P-value:5.73E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:4.91|P-value:1.09E-8 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.69|P-value:4.29E-8 ID:SGTC_167|Compound:3672-0051|FD-Score:-2.98|P-value:3.24E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:2.84|P-value:6.09E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:6.28|P-value:4.16E-13 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.77|P-value:8.26E-6 ID:SGTC_614|Compound:0462-0418|FD-Score:-4.55|P-value:1.01E-7 ID:SGTC_630|Compound:4356-0227|FD-Score:-3.96|P-value:3.08E-6 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.92|P-value:3.83E-6 ID:SGTC_640|Compound:3381-0960|FD-Score:4.74|P-value:3.44E-8 ID:SGTC_652|Compound:0437-0092|FD-Score:-6.36|P-value:1.89E-13 ID:SGTC_697|Compound:0508-1955|FD-Score:2.87|P-value:5.51E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:2.91|P-value:4.69E-4 ID:SGTC_825|Compound:1988-2224|FD-Score:-2.97|P-value:3.35E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:-4.01|P-value:2.32E-6 ID:SGTC_939|Compound:1165-0479|FD-Score:2.93|P-value:4.22E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.21|P-value:1.22E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:2.74|P-value:9.23E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:-3.23|P-value:1.12E-4 ID:SGTC_1198|Compound:1612-1005|FD-Score:2.77|P-value:8.14E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:3.45|P-value:4.24E-5 ID:SGTC_1630|Compound:st005209|FD-Score:2.85|P-value:5.83E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.08|P-value:2.32E-4 ID:SGTC_1779|Compound:st039427|FD-Score:2.75|P-value:8.92E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.01|P-value:2.34E-6 ID:SGTC_1947|Compound:st076332|FD-Score:-3.15|P-value:1.58E-4 ID:SGTC_2093|Compound:5331342|FD-Score:3.46|P-value:4.13E-5 ID:SGTC_2103|Compound:5378138|FD-Score:3.31|P-value:8.29E-5 ID:SGTC_2165|Compound:5727197|FD-Score:-6.30|P-value:3.00E-13 ID:SGTC_2274|Compound:7947094|FD-Score:-2.84|P-value:5.77E-4 ID:SGTC_2293|Compound:7624732|FD-Score:-4.21|P-value:7.59E-7 ID:SGTC_2298|Compound:7976326|FD-Score:-3.05|P-value:2.46E-4 ID:SGTC_2303|Compound:7774051|FD-Score:4.47|P-value:1.83E-7 ID:SGTC_2354|Compound:9060492|FD-Score:-3.18|P-value:1.41E-4 ID:SGTC_2357|Compound:9067248|FD-Score:-2.74|P-value:8.69E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.19|P-value:1.34E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-3.74|P-value:9.38E-6 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.08|P-value:2.31E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.86|P-value:5.69E-6 ID:SGTC_2457|Compound:5731572|FD-Score:2.97|P-value:3.59E-4 ID:SGTC_2458|Compound:5808907|FD-Score:2.95|P-value:3.91E-4 ID:SGTC_2464|Compound:5717415|FD-Score:-2.81|P-value:6.44E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-4.76|P-value:2.61E-8 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-2.80|P-value:6.71E-4 ID:SGTC_2693|Compound:st077581|FD-Score:3.27|P-value:9.84E-5 ID:SGTC_2732|Compound:terfenadine|FD-Score:-2.75|P-value:8.27E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:2.87|P-value:5.44E-4 ID:SGTC_3003|Compound:9075612|FD-Score:2.82|P-value:6.67E-4 ID:SGTC_3018|Compound:9082602|FD-Score:3.48|P-value:3.73E-5

Top fitness defect scores for YDR139C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_310 1273-0058 6.28 1.1 µM 4.16E-13 mitochondrial stress 137 1.69E-10
2 SGTC_163 1327-0036 4.91 7.5 µM 1.09E-8 66 4.47E-7
3 SGTC_640 3381-0960 4.74 37.1 µM 3.44E-8 2 1.09E-6
4 SGTC_2303 7774051 4.47 200.0 µM 1.83E-7 12 3.98E-6
5 SGTC_2415 st077333 3.86 57.7 µM 5.69E-6 48 5.78E-5
6 SGTC_159 3970-0959 3.51 50.0 µM 3.17E-5 108 2.21E-4
7 SGTC_3018 9082602 3.48 49.5 µM 3.73E-5 61 2.51E-4
8 SGTC_2093 5331342 3.46 194.4 µM 4.13E-5 35 2.71E-4
9 SGTC_1520 flindersine 3.45 88.0 µM 4.24E-5 45 2.77E-4
10 SGTC_2103 5378138 3.31 10.0 µM 8.29E-5 40 4.68E-4
11 SGTC_2693 st077581 3.27 40.7 µM 9.84E-5 69 5.35E-4
12 SGTC_2408 sulbentine 3.08 18.4 µM 2.31E-4 91 0.00105
13 SGTC_1679 4130-1276 3.08 6.7 µM 2.32E-4 Golgi 118 0.00105
14 SGTC_2457 5731572 2.97 11.7 µM 3.59E-4 38 0.00148
15 SGTC_2458 5808907 2.95 16.4 µM 3.91E-4 28 0.00158
16 SGTC_939 1165-0479 2.93 45.3 µM 4.22E-4 101 0.00167
17 SGTC_774 k060-0086 2.91 136.0 µM 4.69E-4 66 0.00182
18 SGTC_2736 butenafine 2.87 14.7 µM 5.44E-4 81 0.00205
19 SGTC_697 0508-1955 2.87 564.0 µM 5.51E-4 96 0.00207
20 SGTC_1630 st005209 2.85 31.3 µM 5.83E-4 93 0.00216

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.285 5.81E-64 YDR122W KIN1 hom Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p
0.218 2.66E-37 YKL171W NNK1 hom Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm
0.217 4.23E-37 YER030W CHZ1 hom Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress
0.212 1.62E-35 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.211 4.65E-35 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.207 1.14E-33 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.206 1.71E-33 YEL001C IRC22 hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
0.202 2.32E-32 YGR193C PDX1 hom Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.197 1.20E-30 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
0.194 8.25E-30 YBL066C SEF1 hom Putative transcription factor, has homolog in Kluyveromyces lactis
0.183 8.72E-27 YML074C FPR3 hom Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p
0.177 5.12E-25 YAL061W_p BDH2_p hom Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3
0.169 7.47E-23 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.168 9.05E-23 YDL189W RBS1 hom Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain
0.168 1.28E-22 YGR149W_p YGR149W_p hom Putative protein of unknown function; predicted to be an integal membrane protein