YDR147W / EKI1

Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR147W / EKI1

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:-3.51|P-value:7.21E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.64|P-value:2.63E-7 ID:SGTC_135|Compound:0933-0004|FD-Score:4.82|P-value:1.67E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:-3.53|P-value:6.42E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:3.94|P-value:1.62E-5 ID:SGTC_171|Compound:k064-0041|FD-Score:-3.95|P-value:9.58E-6 ID:SGTC_356|Compound:1634-0012|FD-Score:-2.93|P-value:7.29E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.16|P-value:3.04E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.13|P-value:6.55E-6 ID:SGTC_429|Compound:1112-0049|FD-Score:3.14|P-value:4.84E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:3.09|P-value:5.81E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:-3.51|P-value:7.15E-5 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.19|P-value:3.95E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.18|P-value:4.02E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:3.41|P-value:1.62E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.07|P-value:6.21E-4 ID:SGTC_1503|Compound:4585-0012|FD-Score:-2.93|P-value:7.35E-4 ID:SGTC_1522|Compound:st012842|FD-Score:3.45|P-value:1.41E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:2.97|P-value:8.89E-4 ID:SGTC_1548|Compound:st061468|FD-Score:-3.00|P-value:5.54E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.91|P-value:1.86E-5 ID:SGTC_1786|Compound:berberine|FD-Score:2.99|P-value:8.41E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:3.11|P-value:5.27E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:4.51|P-value:9.13E-7 ID:SGTC_1899|Compound:st063254|FD-Score:3.15|P-value:4.58E-4 ID:SGTC_2053|Compound:5265679|FD-Score:2.96|P-value:9.12E-4 ID:SGTC_2338|Compound:7999974|FD-Score:-3.32|P-value:1.61E-4 ID:SGTC_2372|Compound:9071633|FD-Score:-3.17|P-value:2.84E-4 ID:SGTC_2377|Compound:9073351|FD-Score:-3.48|P-value:7.96E-5 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.31|P-value:2.48E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.55|P-value:9.05E-5 ID:SGTC_2921|Compound:7974240|FD-Score:3.94|P-value:1.56E-5 ID:SGTC_3048|Compound:9093155|FD-Score:-4.10|P-value:4.46E-6 ID:SGTC_3182|Compound:9108849|FD-Score:3.22|P-value:3.50E-4 ID:SGTC_3263|Compound:9137018|FD-Score:5.18|P-value:2.05E-8 ID:SGTC_23|Compound:0213-0011|FD-Score:-3.51|P-value:7.21E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.64|P-value:2.63E-7 ID:SGTC_135|Compound:0933-0004|FD-Score:4.82|P-value:1.67E-7 ID:SGTC_138|Compound:0850-0259|FD-Score:-3.53|P-value:6.42E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:3.94|P-value:1.62E-5 ID:SGTC_171|Compound:k064-0041|FD-Score:-3.95|P-value:9.58E-6 ID:SGTC_356|Compound:1634-0012|FD-Score:-2.93|P-value:7.29E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.16|P-value:3.04E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.13|P-value:6.55E-6 ID:SGTC_429|Compound:1112-0049|FD-Score:3.14|P-value:4.84E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:3.09|P-value:5.81E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:-3.51|P-value:7.15E-5 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.19|P-value:3.95E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.18|P-value:4.02E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:3.41|P-value:1.62E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.07|P-value:6.21E-4 ID:SGTC_1503|Compound:4585-0012|FD-Score:-2.93|P-value:7.35E-4 ID:SGTC_1522|Compound:st012842|FD-Score:3.45|P-value:1.41E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:2.97|P-value:8.89E-4 ID:SGTC_1548|Compound:st061468|FD-Score:-3.00|P-value:5.54E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.91|P-value:1.86E-5 ID:SGTC_1786|Compound:berberine|FD-Score:2.99|P-value:8.41E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:3.11|P-value:5.27E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:4.51|P-value:9.13E-7 ID:SGTC_1899|Compound:st063254|FD-Score:3.15|P-value:4.58E-4 ID:SGTC_2053|Compound:5265679|FD-Score:2.96|P-value:9.12E-4 ID:SGTC_2338|Compound:7999974|FD-Score:-3.32|P-value:1.61E-4 ID:SGTC_2372|Compound:9071633|FD-Score:-3.17|P-value:2.84E-4 ID:SGTC_2377|Compound:9073351|FD-Score:-3.48|P-value:7.96E-5 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.31|P-value:2.48E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.55|P-value:9.05E-5 ID:SGTC_2921|Compound:7974240|FD-Score:3.94|P-value:1.56E-5 ID:SGTC_3048|Compound:9093155|FD-Score:-4.10|P-value:4.46E-6 ID:SGTC_3182|Compound:9108849|FD-Score:3.22|P-value:3.50E-4 ID:SGTC_3263|Compound:9137018|FD-Score:5.18|P-value:2.05E-8

Top fitness defect scores for YDR147W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3263 9137018 5.18 49.5 µM 2.05E-8 2 1.11E-7
2 SGTC_135 0933-0004 4.82 26.4 µM 1.67E-7 60S ribosome export 10 7.11E-7
3 SGTC_1879 gemcitabine 4.51 100.0 µM 9.13E-7 5 3.21E-6
4 SGTC_423 5-fluorouracil 4.13 457.0 µM 6.55E-6 exosome 18 1.85E-5
5 SGTC_2921 7974240 3.94 10.7 µM 1.56E-5 56 4.00E-5
6 SGTC_159 3970-0959 3.94 50.0 µM 1.62E-5 76 4.15E-5
7 SGTC_1780 st038448 3.91 24.5 µM 1.86E-5 iron homeostasis 18 4.68E-5
8 SGTC_2808 7946468 3.55 45.5 µM 9.05E-5 38 1.92E-4
9 SGTC_1522 st012842 3.45 45.6 µM 1.41E-4 RNA pol III & RNase P/MRP 46 2.85E-4
10 SGTC_1303 1160-0052 3.41 261.0 µM 1.62E-4 16 3.22E-4
11 SGTC_2489 cantharidin 3.31 73.4 µM 2.48E-4 cell wall signaling 73 4.73E-4
12 SGTC_3182 9108849 3.22 49.5 µM 3.50E-4 RPP1 & pyrimidine depletion 33 6.42E-4
13 SGTC_1127 asiatic acid 3.19 681.4 nM 3.95E-4 53 7.16E-4
14 SGTC_1230 0170-0365 3.18 3.8 µM 4.02E-4 endomembrane recycling 120 7.26E-4
15 SGTC_1899 st063254 3.15 59.6 µM 4.58E-4 35 8.17E-4
16 SGTC_429 1112-0049 3.14 25.5 µM 4.84E-4 DNA damage response 70 8.59E-4
17 SGTC_1874 staurosporine 3.11 7.6 µM 5.27E-4 cell wall signaling 62 9.26E-4
18 SGTC_561 1550-0330 3.09 72.9 µM 5.81E-4 38 0.00101
19 SGTC_1317 1273-0028 3.07 910.0 nM 6.21E-4 azole & statin 89 0.00107
20 SGTC_1786 berberine 2.99 21.6 µM 8.41E-4 60S ribosome export 93 0.00141

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.146 1.97E-17 YDR446W ECM11 hom Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure
0.146 2.02E-17 YDL128W VCX1 hom Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter
0.136 2.88E-15 YDR480W DIG2 hom MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p
0.120 3.33E-12 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.114 3.68E-11 YDR501W PLM2 hom Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication
0.111 1.07E-10 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.108 3.19E-10 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.107 4.97E-10 YDR196C CAB5 het Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies
0.106 8.58E-10 YGR124W ASN2 hom Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication
0.104 1.44E-9 YGR066C_p YGR066C_p hom Putative protein of unknown function
0.104 1.57E-9 YKL074C MUD2 hom Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65
0.102 3.66E-9 YDR516C EMI2 hom Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.101 4.23E-9 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.098 1.31E-8 YNL323W LEM3 hom Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane
0.094 5.27E-8 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W