HIPHOP chemogenomics database

YDR148C / KGD2

Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR148C / KGD2

Click on Significant Values for Screen Details

Top fitness defect scores for YDR148C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_236	1509-0011	17.90	5.3 µM	1.09E-72		71	1.04E-71
2	SGTC_64	000l-0018	9.45	272.1 µM	9.19E-22	ubiquinone biosynthesis & proteasome	40	1.66E-21
3	SGTC_67	0335-0847	7.96	266.0 µM	5.63E-16	ubiquinone biosynthesis & proteasome	41	8.47E-16
4	SGTC_1635	st007035	7.89	42.4 µM	1.01E-15		98	1.51E-15
5	SGTC_158	k038-0087	5.92	26.4 µM	1.29E-9	ubiquinone biosynthesis & proteasome	70	1.59E-9
6	SGTC_142	0335-0849	5.75	62.7 µM	3.77E-9	ubiquinone biosynthesis & proteasome	66	4.60E-9
7	SGTC_156	3474-0145	5.09	5.6 µM	1.51E-7	endomembrane recycling	31	1.76E-7
8	SGTC_1282	0886-0010	4.69	32.4 µM	1.22E-6	endomembrane recycling	19	1.38E-6
9	SGTC_36	0335-0881	4.43	14.2 µM	4.22E-6	ubiquinone biosynthesis & proteasome	101	4.71E-6
10	SGTC_1795	st049545	4.34	62.3 µM	6.45E-6	TSC3-RPN4	8	7.16E-6
11	SGTC_794	k038-0031	4.32	316.0 µM	7.04E-6	ubiquinone biosynthesis & proteasome	96	7.80E-6
12	SGTC_65	0798-0905	4.12	35.0 µM	1.71E-5	calcium & mitochondrial duress	82	1.88E-5
13	SGTC_216	k038-0035	4.06	28.4 µM	2.28E-5	ubiquinone biosynthesis & proteasome	46	2.49E-5
14	SGTC_2752	diclazuril	3.87	33.1 µM	4.96E-5		121	5.37E-5
15	SGTC_141	0443-0274	3.76	32.3 µM	7.95E-5	mitochondrial response to ROS	48	8.56E-5
16	SGTC_1705	st027868	3.74	56.3 µM	8.72E-5		10	9.39E-5
17	SGTC_367	0335-0881	3.72	7.1 µM	9.24E-5	ubiquinone biosynthesis & proteasome	89	9.93E-5
18	SGTC_386	1509-0011	3.68	3.1 µM	1.10E-4		156	1.19E-4
19	SGTC_852	0418-0143	3.62	439.0 µM	1.37E-4	calcium & mitochondrial duress	86	1.46E-4
20	SGTC_1175	0741-0056	3.61	27.6 µM	1.42E-4		28	1.52E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.682	0	YOR125C	CAT5	hom	Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
0.644	0	YDR204W	COQ4	hom	Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.623	0	YLR201C	COQ9	hom	Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.607	0	YNR041C	COQ2	hom	Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.596	5.63E-322	YNR042W_d	YNR042W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.596	3.85E-321	YGL119W	COQ8	hom	Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.570	7.74E-288	YOL096C	COQ3	hom	O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
0.532	1.17E-244	YJL166W	QCR8	hom	Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.522	3.43E-233	YJR120W	YJR120W	hom	Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.509	7.53E-221	YMR035W	IMP2	hom	Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.503	1.48E-214	YDR230W_d	YDR230W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.499	1.18E-210	YHR050W-A_p	YHR050W-A_p	hom	Protein of unknown function; identified by expression profiling and mass spectrometry
0.499	2.45E-210	YGR255C	COQ6	hom	Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.490	1.14E-201	YOR330C	MIP1	hom	Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.485	1.57E-197	YBR003W	COQ1	hom	Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis