YDR148C / KGD2

Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR148C / KGD2

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:4.43|P-value:4.22E-6 ID:SGTC_64|Compound:000l-0018|FD-Score:9.45|P-value:9.19E-22 ID:SGTC_65|Compound:0798-0905|FD-Score:4.12|P-value:1.71E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:7.96|P-value:5.63E-16 ID:SGTC_73|Compound:0327-0325|FD-Score:3.08|P-value:9.90E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:3.14|P-value:8.03E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.25|P-value:5.49E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:3.76|P-value:7.95E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:5.75|P-value:3.77E-9 ID:SGTC_156|Compound:3474-0145|FD-Score:5.09|P-value:1.51E-7 ID:SGTC_158|Compound:k038-0087|FD-Score:5.92|P-value:1.29E-9 ID:SGTC_216|Compound:k038-0035|FD-Score:4.06|P-value:2.28E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:17.90|P-value:1.09E-72 ID:SGTC_327|Compound:1273-0057|FD-Score:3.39|P-value:3.32E-4 ID:SGTC_343|Compound:4130-2827|FD-Score:3.11|P-value:8.97E-4 ID:SGTC_357|Compound:1325-0123|FD-Score:-4.08|P-value:1.86E-5 ID:SGTC_367|Compound:0335-0881|FD-Score:3.72|P-value:9.24E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:3.68|P-value:1.10E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:3.22|P-value:6.05E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:3.26|P-value:5.29E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:-3.57|P-value:1.53E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:4.32|P-value:7.04E-6 ID:SGTC_852|Compound:0418-0143|FD-Score:3.62|P-value:1.37E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:3.21|P-value:6.27E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:-3.23|P-value:5.52E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.61|P-value:1.42E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:4.69|P-value:1.22E-6 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.16|P-value:7.63E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:-3.10|P-value:8.54E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:-3.39|P-value:3.02E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:-3.09|P-value:9.09E-4 ID:SGTC_1635|Compound:st007035|FD-Score:7.89|P-value:1.01E-15 ID:SGTC_1705|Compound:st027868|FD-Score:3.74|P-value:8.72E-5 ID:SGTC_1767|Compound:st044992|FD-Score:3.15|P-value:7.84E-4 ID:SGTC_1795|Compound:st049545|FD-Score:4.34|P-value:6.45E-6 ID:SGTC_1945|Compound:st075991|FD-Score:3.44|P-value:2.76E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.87|P-value:4.96E-5 ID:SGTC_2796|Compound:7949634|FD-Score:3.27|P-value:5.08E-4 ID:SGTC_2935|Compound:9038016|FD-Score:3.13|P-value:8.32E-4 ID:SGTC_2938|Compound:9059746|FD-Score:-3.49|P-value:2.09E-4 ID:SGTC_3099|Compound:9120367|FD-Score:3.42|P-value:2.98E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-3.97|P-value:3.06E-5 ID:SGTC_3309|Compound:9129340|FD-Score:-3.66|P-value:1.07E-4 ID:SGTC_3340|Compound:9146634|FD-Score:3.13|P-value:8.44E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:4.43|P-value:4.22E-6 ID:SGTC_64|Compound:000l-0018|FD-Score:9.45|P-value:9.19E-22 ID:SGTC_65|Compound:0798-0905|FD-Score:4.12|P-value:1.71E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:7.96|P-value:5.63E-16 ID:SGTC_73|Compound:0327-0325|FD-Score:3.08|P-value:9.90E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:3.14|P-value:8.03E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.25|P-value:5.49E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:3.76|P-value:7.95E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:5.75|P-value:3.77E-9 ID:SGTC_156|Compound:3474-0145|FD-Score:5.09|P-value:1.51E-7 ID:SGTC_158|Compound:k038-0087|FD-Score:5.92|P-value:1.29E-9 ID:SGTC_216|Compound:k038-0035|FD-Score:4.06|P-value:2.28E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:17.90|P-value:1.09E-72 ID:SGTC_327|Compound:1273-0057|FD-Score:3.39|P-value:3.32E-4 ID:SGTC_343|Compound:4130-2827|FD-Score:3.11|P-value:8.97E-4 ID:SGTC_357|Compound:1325-0123|FD-Score:-4.08|P-value:1.86E-5 ID:SGTC_367|Compound:0335-0881|FD-Score:3.72|P-value:9.24E-5 ID:SGTC_386|Compound:1509-0011|FD-Score:3.68|P-value:1.10E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:3.22|P-value:6.05E-4 ID:SGTC_741|Compound:4582-0002|FD-Score:3.26|P-value:5.29E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:-3.57|P-value:1.53E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:4.32|P-value:7.04E-6 ID:SGTC_852|Compound:0418-0143|FD-Score:3.62|P-value:1.37E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:3.21|P-value:6.27E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:-3.23|P-value:5.52E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.61|P-value:1.42E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:4.69|P-value:1.22E-6 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.16|P-value:7.63E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:-3.10|P-value:8.54E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:-3.39|P-value:3.02E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:-3.09|P-value:9.09E-4 ID:SGTC_1635|Compound:st007035|FD-Score:7.89|P-value:1.01E-15 ID:SGTC_1705|Compound:st027868|FD-Score:3.74|P-value:8.72E-5 ID:SGTC_1767|Compound:st044992|FD-Score:3.15|P-value:7.84E-4 ID:SGTC_1795|Compound:st049545|FD-Score:4.34|P-value:6.45E-6 ID:SGTC_1945|Compound:st075991|FD-Score:3.44|P-value:2.76E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.87|P-value:4.96E-5 ID:SGTC_2796|Compound:7949634|FD-Score:3.27|P-value:5.08E-4 ID:SGTC_2935|Compound:9038016|FD-Score:3.13|P-value:8.32E-4 ID:SGTC_2938|Compound:9059746|FD-Score:-3.49|P-value:2.09E-4 ID:SGTC_3099|Compound:9120367|FD-Score:3.42|P-value:2.98E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-3.97|P-value:3.06E-5 ID:SGTC_3309|Compound:9129340|FD-Score:-3.66|P-value:1.07E-4 ID:SGTC_3340|Compound:9146634|FD-Score:3.13|P-value:8.44E-4

Top fitness defect scores for YDR148C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 17.90 5.3 µM 1.09E-72 71 1.04E-71
2 SGTC_64 000l-0018 9.45 272.1 µM 9.19E-22 ubiquinone biosynthesis & proteasome 40 1.66E-21
3 SGTC_67 0335-0847 7.96 266.0 µM 5.63E-16 ubiquinone biosynthesis & proteasome 41 8.47E-16
4 SGTC_1635 st007035 7.89 42.4 µM 1.01E-15 98 1.51E-15
5 SGTC_158 k038-0087 5.92 26.4 µM 1.29E-9 ubiquinone biosynthesis & proteasome 70 1.59E-9
6 SGTC_142 0335-0849 5.75 62.7 µM 3.77E-9 ubiquinone biosynthesis & proteasome 66 4.60E-9
7 SGTC_156 3474-0145 5.09 5.6 µM 1.51E-7 endomembrane recycling 31 1.76E-7
8 SGTC_1282 0886-0010 4.69 32.4 µM 1.22E-6 endomembrane recycling 19 1.38E-6
9 SGTC_36 0335-0881 4.43 14.2 µM 4.22E-6 ubiquinone biosynthesis & proteasome 101 4.71E-6
10 SGTC_1795 st049545 4.34 62.3 µM 6.45E-6 TSC3-RPN4 8 7.16E-6
11 SGTC_794 k038-0031 4.32 316.0 µM 7.04E-6 ubiquinone biosynthesis & proteasome 96 7.80E-6
12 SGTC_65 0798-0905 4.12 35.0 µM 1.71E-5 calcium & mitochondrial duress 82 1.88E-5
13 SGTC_216 k038-0035 4.06 28.4 µM 2.28E-5 ubiquinone biosynthesis & proteasome 46 2.49E-5
14 SGTC_2752 diclazuril 3.87 33.1 µM 4.96E-5 121 5.37E-5
15 SGTC_141 0443-0274 3.76 32.3 µM 7.95E-5 mitochondrial response to ROS 48 8.56E-5
16 SGTC_1705 st027868 3.74 56.3 µM 8.72E-5 10 9.39E-5
17 SGTC_367 0335-0881 3.72 7.1 µM 9.24E-5 ubiquinone biosynthesis & proteasome 89 9.93E-5
18 SGTC_386 1509-0011 3.68 3.1 µM 1.10E-4 156 1.19E-4
19 SGTC_852 0418-0143 3.62 439.0 µM 1.37E-4 calcium & mitochondrial duress 86 1.46E-4
20 SGTC_1175 0741-0056 3.61 27.6 µM 1.42E-4 28 1.52E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.682 0 YOR125C CAT5 hom Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
0.644 0 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.623 0 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.607 0 YNR041C COQ2 hom Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.596 5.63E-322 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.596 3.85E-321 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.570 7.74E-288 YOL096C COQ3 hom O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
0.532 1.17E-244 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.522 3.43E-233 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.509 7.53E-221 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.503 1.48E-214 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.499 1.18E-210 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.499 2.45E-210 YGR255C COQ6 hom Putative flavin-dependent monooxygenase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human COX6 can rescue a yeast cox6 mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
0.490 1.14E-201 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.485 1.57E-197 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis