HIPHOP chemogenomics database

YDR162C / NBP2

Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR162C / NBP2

Click on Significant Values for Screen Details

Top fitness defect scores for YDR162C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_964	1287-0107	39.70	19.4 µM	1.21E-197	cell wall	1	0
2	SGTC_2912	7964298	12.30	10.9 µM	1.29E-20	cell wall	10	3.56E-35
3	SGTC_2688	kanamycin b	10.70	96.7 µM	4.87E-16	calcium & mitochondrial duress	19	3.83E-27
4	SGTC_439	amiodarone	10.70	34.4 µM	7.18E-16	NEO1-PIK1	12	7.55E-27
5	SGTC_3219	9131112	10.10	49.5 µM	2.54E-14	Golgi	4	3.95E-24
6	SGTC_966	1300-0360	9.20	30.2 µM	3.09E-12	heme requiring	2	1.79E-20
7	SGTC_2526	patulin	9.16	37.6 µM	3.84E-12		10	2.62E-20
8	SGTC_71	0417-1750	9.08	47.8 µM	5.96E-12	Golgi	7	5.67E-20
9	SGTC_2781	5567276	8.77	71.4 µM	2.85E-11		5	8.83E-19
10	SGTC_934	2950-0684	8.61	338.0 µM	6.43E-11		1	3.67E-18
11	SGTC_958	1222-0007	8.45	33.0 µM	1.43E-10		1	1.49E-17
12	SGTC_928	2764-0428	8.42	58.7 µM	1.61E-10	cell wall	6	1.84E-17
13	SGTC_879	0831-1030	8.42	162.0 µM	1.62E-10		6	1.85E-17
14	SGTC_1844	rapamycin	8.04	4.0 nM	9.87E-10		56	4.40E-16
15	SGTC_3204	9114183	7.93	49.5 µM	1.68E-9		3	1.12E-15
16	SGTC_1760	st045453	7.66	10.4 µM	5.57E-9	NEO1-PIK1	58	9.12E-15
17	SGTC_221	trifluoperazine	7.60	10.3 µM	7.47E-9	NEO1-PIK1	90	1.53E-14
18	SGTC_2619	obliquin	7.29	87.9 µM	2.83E-8	cell wall	52	1.57E-13
19	SGTC_1797	st048695	7.28	56.8 µM	2.90E-8	NEO1	27	1.64E-13
20	SGTC_360	0957-0399	7.22	1.0 µM	3.70E-8	cell wall	8	2.52E-13

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.252	1.26E-49	YDL006W	PTC1	hom	Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation
0.237	7.24E-44	YBR023C	CHS3	hom	Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.236	1.39E-43	YDR389W	SAC7	hom	GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate
0.232	3.83E-42	YPL066W	RGL1	hom	Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.228	9.81E-41	YPL065W	VPS28	hom	Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.228	1.43E-40	YBL061C	SKT5	hom	Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
0.227	2.22E-40	YJR102C	VPS25	hom	Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
0.222	1.44E-38	YAL009W	SPO7	hom	Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation
0.220	5.01E-38	YPL002C	SNF8	hom	Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
0.215	2.89E-36	YCL008C	STP22	hom	Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype
0.211	3.99E-35	YJL053W	PEP8	hom	Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.211	8.43E-35	YHR004C	NEM1	hom	Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard
0.209	2.03E-34	YJL099W	CHS6	hom	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.209	2.65E-34	YJL124C	LSM1	hom	Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
0.209	3.00E-34	YLR025W	SNF7	hom	One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes