HIPHOP chemogenomics database

YDR186C

Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR186C / YDR186C

Click on Significant Values for Screen Details

Top fitness defect scores for YDR186C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1161	k007-0610	15.90	110.0 µM	1.14E-57	ERAD & cell cycle	4	3.26E-57
2	SGTC_733	4069-0007	12.30	56.1 µM	2.48E-35	ERAD & cell cycle	9	5.32E-35
3	SGTC_103	0844-0104	12.20	51.8 µM	8.06E-35	ERAD & cell cycle	16	1.72E-34
4	SGTC_384	k007-0144	10.90	5.2 µM	1.81E-28	ERAD & cell cycle	9	3.51E-28
5	SGTC_373	0844-0104	9.79	25.9 µM	3.41E-23	ERAD & cell cycle	10	6.10E-23
6	SGTC_451	chloral hydrate	8.09	100.0 µM	1.82E-16		5	2.91E-16
7	SGTC_1736	st036774	6.85	51.0 µM	2.48E-12		4	3.65E-12
8	SGTC_568	1193-0097	6.53	7.5 µM	2.22E-11	ERAD & cell cycle	53	3.20E-11
9	SGTC_805	3852-0218	6.35	479.0 µM	7.70E-11	ERAD & cell cycle	22	1.10E-10
10	SGTC_2582	agaric acid	5.45	100.0 µM	1.87E-8	redox potentiating	193	2.53E-8
11	SGTC_547	1120-0019	4.91	610.0 nM	3.47E-7	azole & statin	17	4.56E-7
12	SGTC_1658	st012944	4.77	20.0 µM	7.11E-7	iron homeostasis	53	9.25E-7
13	SGTC_1094	duloxetine	4.75	25.4 µM	7.77E-7	RPP1 & pyrimidine depletion	5	1.01E-6
14	SGTC_2130	NSC 7	4.66	2.5 µM	1.20E-6	copper-dependent oxidative stress	12	1.56E-6
15	SGTC_1597	7-benzyloxygramine	4.66	47.5 µM	1.20E-6	calcium & mitochondrial duress	79	1.56E-6
16	SGTC_2285	7945142	4.63	200.0 µM	1.40E-6		10	1.81E-6
17	SGTC_424	splitomicin	4.19	100.0 mM	1.10E-5		16	1.39E-5
18	SGTC_67	0335-0847	4.11	266.0 µM	1.60E-5	ubiquinone biosynthesis & proteasome	111	2.01E-5
19	SGTC_352	k048-0007	4.09	5.1 µM	1.70E-5	ERAD & cell cycle	73	2.13E-5
20	SGTC_2085	5307070	4.07	144.1 µM	1.85E-5	superoxide	25	2.32E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.628	0	YIL030C	SSM4	hom	Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.518	6.56E-230	YOL002C	IZH2	hom	Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.490	1.42E-202	YER084W_p	YER084W_p	hom	Protein of unknown function; expressed at both mRNA and protein levels
0.486	2.87E-198	YDR199W_d	YDR199W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.465	9.10E-180	YMR029C	FAR8	hom	Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.418	5.67E-142	YDL148C	NOP14	het	Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.410	1.85E-136	YMR022W	UBC7	hom	Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.397	2.56E-127	YMR052C-A_d	YMR052C-A_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.387	1.62E-120	YMR052W	FAR3	hom	Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.383	1.77E-117	YKR014C	YPT52	hom	Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress
0.346	6.61E-95	YFR008W	FAR7	hom	Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
0.344	4.15E-94	YLR380W	CSR1	hom	Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.333	1.64E-87	YOR246C	ENV9	hom	Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.328	5.26E-85	YOL065C	INP54	hom	Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.327	2.39E-84	YNL287W	SEC21	het	Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo