YDR189W / SLY1

Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR189W / SLY1

Click on Significant Values for Screen Details ID:SGTC_68|Compound:0986-0284|FD-Score:-3.24|P-value:9.11E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:11.50|P-value:7.89E-29 ID:SGTC_89|Compound:0302-0250|FD-Score:3.44|P-value:4.22E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-4.42|P-value:1.01E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:3.20|P-value:9.59E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:3.48|P-value:3.73E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:3.90|P-value:7.88E-5 ID:SGTC_726|Compound:3448-4391|FD-Score:4.79|P-value:1.79E-6 ID:SGTC_934|Compound:2950-0684|FD-Score:4.32|P-value:1.46E-5 ID:SGTC_1056|Compound:3448-1962|FD-Score:4.26|P-value:1.87E-5 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.69|P-value:1.73E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:-3.53|P-value:3.31E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:-3.25|P-value:8.74E-4 ID:SGTC_1626|Compound:st003712|FD-Score:3.63|P-value:2.15E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.39|P-value:5.13E-4 ID:SGTC_1899|Compound:st063254|FD-Score:3.23|P-value:8.74E-4 ID:SGTC_1943|Compound:st076331|FD-Score:3.39|P-value:5.16E-4 ID:SGTC_1952|Compound:st077036|FD-Score:-3.31|P-value:7.22E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-3.39|P-value:5.46E-4 ID:SGTC_2394|Compound:5221648|FD-Score:4.47|P-value:7.34E-6 ID:SGTC_2398|Compound:5228213|FD-Score:3.92|P-value:7.26E-5 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.30|P-value:7.01E-4 ID:SGTC_2804|Compound:7954240|FD-Score:4.63|P-value:3.61E-6 ID:SGTC_2810|Compound:7959871|FD-Score:3.26|P-value:7.77E-4 ID:SGTC_2905|Compound:7799065|FD-Score:4.71|P-value:2.53E-6 ID:SGTC_3013|Compound:9080133|FD-Score:3.26|P-value:7.77E-4 ID:SGTC_3026|Compound:9090633|FD-Score:4.87|P-value:1.20E-6 ID:SGTC_3108|Compound:9121785|FD-Score:3.55|P-value:2.94E-4 ID:SGTC_3139|Compound:9095020|FD-Score:-4.39|P-value:1.16E-5 ID:SGTC_3144|Compound:9087057|FD-Score:-4.86|P-value:1.37E-6 ID:SGTC_3178|Compound:9107506|FD-Score:-3.85|P-value:1.01E-4 ID:SGTC_3187|Compound:9109394|FD-Score:3.65|P-value:2.04E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:-3.24|P-value:9.11E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:11.50|P-value:7.89E-29 ID:SGTC_89|Compound:0302-0250|FD-Score:3.44|P-value:4.22E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-4.42|P-value:1.01E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:3.20|P-value:9.59E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:3.48|P-value:3.73E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:3.90|P-value:7.88E-5 ID:SGTC_726|Compound:3448-4391|FD-Score:4.79|P-value:1.79E-6 ID:SGTC_934|Compound:2950-0684|FD-Score:4.32|P-value:1.46E-5 ID:SGTC_1056|Compound:3448-1962|FD-Score:4.26|P-value:1.87E-5 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.69|P-value:1.73E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:-3.53|P-value:3.31E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:-3.25|P-value:8.74E-4 ID:SGTC_1626|Compound:st003712|FD-Score:3.63|P-value:2.15E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.39|P-value:5.13E-4 ID:SGTC_1899|Compound:st063254|FD-Score:3.23|P-value:8.74E-4 ID:SGTC_1943|Compound:st076331|FD-Score:3.39|P-value:5.16E-4 ID:SGTC_1952|Compound:st077036|FD-Score:-3.31|P-value:7.22E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-3.39|P-value:5.46E-4 ID:SGTC_2394|Compound:5221648|FD-Score:4.47|P-value:7.34E-6 ID:SGTC_2398|Compound:5228213|FD-Score:3.92|P-value:7.26E-5 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.30|P-value:7.01E-4 ID:SGTC_2804|Compound:7954240|FD-Score:4.63|P-value:3.61E-6 ID:SGTC_2810|Compound:7959871|FD-Score:3.26|P-value:7.77E-4 ID:SGTC_2905|Compound:7799065|FD-Score:4.71|P-value:2.53E-6 ID:SGTC_3013|Compound:9080133|FD-Score:3.26|P-value:7.77E-4 ID:SGTC_3026|Compound:9090633|FD-Score:4.87|P-value:1.20E-6 ID:SGTC_3108|Compound:9121785|FD-Score:3.55|P-value:2.94E-4 ID:SGTC_3139|Compound:9095020|FD-Score:-4.39|P-value:1.16E-5 ID:SGTC_3144|Compound:9087057|FD-Score:-4.86|P-value:1.37E-6 ID:SGTC_3178|Compound:9107506|FD-Score:-3.85|P-value:1.01E-4 ID:SGTC_3187|Compound:9109394|FD-Score:3.65|P-value:2.04E-4

Top fitness defect scores for YDR189W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_81 0411-0046 11.50 10.0 µM 7.89E-29 5 1.12E-30 0.66
2 SGTC_3026 9090633 4.87 49.5 µM 1.20E-6 3 5.54E-7 0.23
3 SGTC_726 3448-4391 4.79 34.2 µM 1.79E-6 1 8.53E-7 0.58
4 SGTC_2905 7799065 4.71 20.1 µM 2.53E-6 5 1.23E-6 0.41
5 SGTC_2804 7954240 4.63 19.5 µM 3.61E-6 7 1.80E-6 0.02
6 SGTC_2394 5221648 4.47 200.0 µM 7.34E-6 4 3.83E-6 0.11
7 SGTC_934 2950-0684 4.32 338.0 µM 1.46E-5 4 7.98E-6 0.60
8 SGTC_1056 3448-1962 4.26 115.0 µM 1.87E-5 amide catabolism 6 1.04E-5 0.40
9 SGTC_2398 5228213 3.92 48.8 µM 7.26E-5 iron homeostasis 13 4.40E-5 0.14
10 SGTC_623 k072-0108 3.90 16.9 µM 7.88E-5 heme biosynthesis & mitochondrial translocase 15 4.80E-5 0.28
11 SGTC_1293 1000-1278 3.69 195.0 µM 1.73E-4 10 1.11E-4 0.15
12 SGTC_3187 9109394 3.65 49.5 µM 2.04E-4 Golgi 13 1.32E-4 0.02
13 SGTC_1626 st003712 3.63 1.8 µM 2.15E-4 13 1.40E-4 0.18
14 SGTC_3108 9121785 3.55 49.5 µM 2.94E-4 18 1.95E-4 0.34
15 SGTC_273 hydroxyurea 3.48 18.1 mM 3.73E-4 heme biosynthesis & mitochondrial translocase 28 2.51E-4 0.00
16 SGTC_89 0302-0250 3.44 142.1 µM 4.22E-4 6 2.86E-4 0.11
17 SGTC_1728 st035614 3.39 33.8 µM 5.13E-4 11 3.52E-4 0.07
18 SGTC_1943 st076331 3.39 65.2 µM 5.16E-4 4 3.55E-4 0.08
19 SGTC_2639 lactacystin 3.30 53.0 µM 7.01E-4 15 4.91E-4 0.00
20 SGTC_3013 9080133 3.26 71.4 µM 7.77E-4 6 5.47E-4 0.13

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.183 8.84E-27 YDR272W GLO2 hom Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate
0.167 2.06E-22 YMR260C TIF11 het Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
0.140 3.04E-16 YDR041W RSM10 het Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
0.130 3.85E-14 YNL114C_d YNL114C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
0.124 5.86E-13 YPR094W RDS3 het Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance
0.120 3.51E-12 YOL146W PSF3 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.119 4.32E-12 YOL049W GSH2 hom Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
0.118 6.33E-12 YLR147C SMD3 het Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3
0.118 7.74E-12 YBR109C CMD1 het Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
0.116 1.49E-11 YBR239C_p ERT1_p hom Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition
0.116 1.81E-11 YDR002W YRB1 het Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
0.115 2.34E-11 YML004C GLO1 hom Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress
0.115 2.92E-11 YPL128C TBF1 het Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
0.110 1.79E-10 YPR105C COG4 het Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.107 5.49E-10 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair