YDR208W / MSS4

Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR208W / MSS4

Click on Significant Values for Screen Details ID:SGTC_49|Compound:0717-0965|FD-Score:3.79|P-value:8.24E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:7.79|P-value:3.98E-19 ID:SGTC_266|Compound:formosanin C|FD-Score:5.74|P-value:3.34E-11 ID:SGTC_364|Compound:0083-0097|FD-Score:3.38|P-value:6.06E-5 ID:SGTC_500|Compound:h-89|FD-Score:-2.84|P-value:6.02E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:-2.93|P-value:4.16E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:4.08|P-value:1.77E-6 ID:SGTC_637|Compound:1181-0519|FD-Score:-2.80|P-value:7.03E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:2.92|P-value:4.55E-4 ID:SGTC_721|Compound:1187-1332|FD-Score:-3.44|P-value:4.50E-5 ID:SGTC_746|Compound:1095-0025|FD-Score:2.75|P-value:8.70E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:3.07|P-value:2.43E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:5.24|P-value:1.26E-9 ID:SGTC_804|Compound:1595-0054|FD-Score:3.02|P-value:3.01E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:2.96|P-value:3.75E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.68|P-value:1.41E-5 ID:SGTC_883|Compound:0850-0245|FD-Score:-3.20|P-value:1.34E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:2.75|P-value:8.86E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:3.72|P-value:1.14E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.83|P-value:6.29E-6 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.15|P-value:1.73E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.76|P-value:9.58E-6 ID:SGTC_1304|Compound:1165-0502|FD-Score:2.84|P-value:6.13E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:2.76|P-value:8.59E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:4.13|P-value:1.29E-6 ID:SGTC_1366|Compound:1683-0113|FD-Score:-2.76|P-value:8.40E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:4.19|P-value:9.43E-7 ID:SGTC_1724|Compound:st037281|FD-Score:-3.33|P-value:7.28E-5 ID:SGTC_1920|Compound:st075728|FD-Score:3.56|P-value:2.55E-5 ID:SGTC_1923|Compound:st058458|FD-Score:3.43|P-value:4.79E-5 ID:SGTC_1924|Compound:st058459|FD-Score:4.14|P-value:1.24E-6 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:2.77|P-value:8.30E-4 ID:SGTC_2095|Compound:5432005|FD-Score:-2.84|P-value:5.96E-4 ID:SGTC_2125|Compound:5246583|FD-Score:3.68|P-value:1.40E-5 ID:SGTC_2130|Compound:NSC 7|FD-Score:7.31|P-value:4.32E-17 ID:SGTC_2135|Compound:5326629|FD-Score:-3.62|P-value:1.81E-5 ID:SGTC_2160|Compound:5660466|FD-Score:3.61|P-value:2.04E-5 ID:SGTC_2248|Compound:tofa|FD-Score:3.99|P-value:2.84E-6 ID:SGTC_2396|Compound:5342950|FD-Score:3.36|P-value:6.55E-5 ID:SGTC_2601|Compound:securinine|FD-Score:-3.14|P-value:1.72E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.16|P-value:1.14E-6 ID:SGTC_2781|Compound:5567276|FD-Score:2.80|P-value:7.29E-4 ID:SGTC_2783|Compound:7738165|FD-Score:3.65|P-value:1.61E-5 ID:SGTC_2802|Compound:7925815|FD-Score:-3.89|P-value:4.66E-6 ID:SGTC_2928|Compound:9011484|FD-Score:2.73|P-value:9.50E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-2.76|P-value:8.37E-4 ID:SGTC_2983|Compound:9022671|FD-Score:3.03|P-value:2.82E-4 ID:SGTC_3020|Compound:9089140|FD-Score:-2.90|P-value:4.82E-4 ID:SGTC_3078|Compound:9116413|FD-Score:-2.76|P-value:8.13E-4 ID:SGTC_3202|Compound:9113761|FD-Score:2.94|P-value:4.10E-4 ID:SGTC_3219|Compound:9131112|FD-Score:3.26|P-value:1.06E-4 ID:SGTC_3244|Compound:9134489|FD-Score:2.81|P-value:6.90E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:3.79|P-value:8.24E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:7.79|P-value:3.98E-19 ID:SGTC_266|Compound:formosanin C|FD-Score:5.74|P-value:3.34E-11 ID:SGTC_364|Compound:0083-0097|FD-Score:3.38|P-value:6.06E-5 ID:SGTC_500|Compound:h-89|FD-Score:-2.84|P-value:6.02E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:-2.93|P-value:4.16E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:4.08|P-value:1.77E-6 ID:SGTC_637|Compound:1181-0519|FD-Score:-2.80|P-value:7.03E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:2.92|P-value:4.55E-4 ID:SGTC_721|Compound:1187-1332|FD-Score:-3.44|P-value:4.50E-5 ID:SGTC_746|Compound:1095-0025|FD-Score:2.75|P-value:8.70E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:3.07|P-value:2.43E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:5.24|P-value:1.26E-9 ID:SGTC_804|Compound:1595-0054|FD-Score:3.02|P-value:3.01E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:2.96|P-value:3.75E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.68|P-value:1.41E-5 ID:SGTC_883|Compound:0850-0245|FD-Score:-3.20|P-value:1.34E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:2.75|P-value:8.86E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:3.72|P-value:1.14E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.83|P-value:6.29E-6 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.15|P-value:1.73E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.76|P-value:9.58E-6 ID:SGTC_1304|Compound:1165-0502|FD-Score:2.84|P-value:6.13E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:2.76|P-value:8.59E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:4.13|P-value:1.29E-6 ID:SGTC_1366|Compound:1683-0113|FD-Score:-2.76|P-value:8.40E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:4.19|P-value:9.43E-7 ID:SGTC_1724|Compound:st037281|FD-Score:-3.33|P-value:7.28E-5 ID:SGTC_1920|Compound:st075728|FD-Score:3.56|P-value:2.55E-5 ID:SGTC_1923|Compound:st058458|FD-Score:3.43|P-value:4.79E-5 ID:SGTC_1924|Compound:st058459|FD-Score:4.14|P-value:1.24E-6 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:2.77|P-value:8.30E-4 ID:SGTC_2095|Compound:5432005|FD-Score:-2.84|P-value:5.96E-4 ID:SGTC_2125|Compound:5246583|FD-Score:3.68|P-value:1.40E-5 ID:SGTC_2130|Compound:NSC 7|FD-Score:7.31|P-value:4.32E-17 ID:SGTC_2135|Compound:5326629|FD-Score:-3.62|P-value:1.81E-5 ID:SGTC_2160|Compound:5660466|FD-Score:3.61|P-value:2.04E-5 ID:SGTC_2248|Compound:tofa|FD-Score:3.99|P-value:2.84E-6 ID:SGTC_2396|Compound:5342950|FD-Score:3.36|P-value:6.55E-5 ID:SGTC_2601|Compound:securinine|FD-Score:-3.14|P-value:1.72E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.16|P-value:1.14E-6 ID:SGTC_2781|Compound:5567276|FD-Score:2.80|P-value:7.29E-4 ID:SGTC_2783|Compound:7738165|FD-Score:3.65|P-value:1.61E-5 ID:SGTC_2802|Compound:7925815|FD-Score:-3.89|P-value:4.66E-6 ID:SGTC_2928|Compound:9011484|FD-Score:2.73|P-value:9.50E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-2.76|P-value:8.37E-4 ID:SGTC_2983|Compound:9022671|FD-Score:3.03|P-value:2.82E-4 ID:SGTC_3020|Compound:9089140|FD-Score:-2.90|P-value:4.82E-4 ID:SGTC_3078|Compound:9116413|FD-Score:-2.76|P-value:8.13E-4 ID:SGTC_3202|Compound:9113761|FD-Score:2.94|P-value:4.10E-4 ID:SGTC_3219|Compound:9131112|FD-Score:3.26|P-value:1.06E-4 ID:SGTC_3244|Compound:9134489|FD-Score:2.81|P-value:6.90E-4

Top fitness defect scores for YDR208W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_58 0957-0393 7.79 9.0 µM 3.98E-19 cell wall 18 3.45E-15 0.01
2 SGTC_2130 NSC 7 7.31 2.5 µM 4.32E-17 copper-dependent oxidative stress 1 1.30E-13 1.90
3 SGTC_266 formosanin C 5.74 406.0 nM 3.34E-11 sphingolipid biosynthesis & PDR1 9 4.81E-9 0.08
4 SGTC_801 0866-0028 5.24 10.1 µM 1.26E-9 4 8.06E-8 0.49
5 SGTC_1568 digitonin 4.19 820.0 nM 9.43E-7 sphingolipid biosynthesis & PDR1 12 1.40E-5 0.04
6 SGTC_2640 pentamidine 4.16 10.4 µM 1.14E-6 mitochondrial processes 14 1.62E-5 0.17
7 SGTC_1924 st058459 4.14 75.1 µM 1.24E-6 6 1.74E-5 0.19
8 SGTC_1357 1503-1303 4.13 39.5 µM 1.29E-6 6 1.79E-5 0.03
9 SGTC_628 1636-0253 4.08 16.0 µM 1.77E-6 sphingolipid biosynthesis & PDR1 8 2.28E-5 0.02
10 SGTC_2248 tofa 3.99 1.1 µM 2.84E-6 sphingolipid biosynthesis & PDR1 28 3.30E-5 0.11
11 SGTC_49 0717-0965 3.79 32.1 µM 8.24E-6 60S ribosome export 9 7.60E-5 0.01
12 SGTC_1254 0550-0035 3.76 264.0 µM 9.58E-6 9 8.56E-5 0.06
13 SGTC_994 3910-0338 3.72 138.0 µM 1.14E-5 calcium & mitochondrial duress 17 9.82E-5 0.00
14 SGTC_2125 5246583 3.68 200.0 µM 1.40E-5 tubulin folding & SWR complex 6 1.15E-4 0.04
15 SGTC_811 0416-0114 3.68 265.0 µM 1.41E-5 3 1.16E-4 0.09
16 SGTC_2783 7738165 3.65 71.4 µM 1.61E-5 8 1.29E-4 0.21
17 SGTC_2160 5660466 3.61 132.9 µM 2.04E-5 60S ribosome export 19 1.54E-4 0.03
18 SGTC_1920 st075728 3.56 45.7 µM 2.55E-5 7 1.84E-4 0.11
19 SGTC_1923 st058458 3.43 19.1 µM 4.79E-5 15 3.02E-4 0.12
20 SGTC_364 0083-0097 3.38 67.7 µM 6.06E-5 12 3.63E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.257 1.27E-51 YMR102C_p YMR102C_p hom Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.232 3.78E-42 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.232 4.29E-42 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.218 2.35E-37 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.215 1.80E-36 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.215 2.75E-36 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.203 1.83E-32 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.195 5.28E-30 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.191 8.50E-29 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.188 4.15E-28 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.187 7.12E-28 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.182 2.55E-26 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.177 3.83E-25 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.177 5.95E-25 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.175 1.65E-24 YHR073W OSH3 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability